Henry Daniell, Ph.D.

faculty photo
Professor of Biochemistry
Department: Biochemistry
Graduate Group Affiliations

Contact information
547 Levy, 240 south 40th Street
Philadelphia, PA 19104-6030
Office: 1-215-746-2563
Fax: 1-215-898-3695
Education:
BS (Botany/Chemistry)
University of Madras, 1969.
MS (Botany)
University of Madras, 1971.
PhD (Biochemistry)
Madurai Kamaraj University, 1980.
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Description of Research Expertise

Research Interests: Oral delivery of human therapeutic proteins bio-encapsulated within plant cells to confer protection against inherited or infectious diseases

Key Words: Oral delivery, bioencapsulation in plant cells, vaccine antigens, autoimmune disorders, infectious or inherited diseases


In an emerging new concept, freeze-dried plant cells (lettuce) expressing biopharmaceuticals (fused with transmucosal carriers) are protected in the stomach from acids/enzymes but are released to the circulatory system when plant cell walls are digested by microbes that colonize the gut. Therapeutic proteins in lyophilized plant cells (in capsules) are stable >15 months at room temperature and maintain their folding, disulfide bonds, assembly and functionality. Furthermore, lyophilization process increases the antigen concentration up to 25-fold and eliminates microbes in harvested leaf materials.

Oral delivery of antigens or auto-antigens bio-encapsulated in plant cells:Several vaccine antigens (against Dengue, polio, malaria, tuberculosis, cholera, anthrax, plague, etc) or autoantigens (diabetes, hemophilia, etc) have been expressed in lettuce chloroplasts. Vaccine antigens bio-encapsulated in plant cells upon oral delivery after priming, conferred both mucosal and systemic immunity and protection against bacterial, viral or protozoan pathogens or toxin challenge. Oral delivery of autoantigens was effective in delaying the onset of type 1 diabetes or inhibitory antibodies/complications after injection of blood clotting factors in hemophiliaA/B, by developing tolerance.


Oral delivery of functional proteins: Oral delivery of exendin-4 expressed in plant cells regulated blood glucose levels similar to injections by stimulating insulin secretion and a 5,000 fold excess dose didn’t cause hypoglycemia because insulinotropism of exendin-4 is glucose dependent. Oral delivery of angiotensin converting enzyme 2 (ACE2) or angiotensin-(1-7) attenuated pulmonary hypertension and associated cardiac dysfunction. Amyloid plaques were reduced in vivo by 70% in hippocampus and cortex brain regions when fed with myelin basic protein and reduced Aβ42 accumulation in retinae and prevented loss of retinal ganglion cells in 3xTg Alzheimer’s disease mice.

Therefore, this new platform should offer a low cost alternative to deliver human therapeutic proteins to combat infectious or inherited diseases by eliminating inactivated pathogens, expensive purification, cold storage/transportation and sterile injections.


Rotation projects:

Low cost oral polio booster vaccine (funded by the Bill and Melinda Gates Foundation)

Oral immune modulatory therapy using antigens bio-encapsulated in plant cells (funded by the National Institutes of Health)

Oral therapy for hemophilia A (funded by the National Institutes of Health)

Preventing type 1diabetes by oral delivery of plant-derived auto-antigens (funded by the Juvenile Diabetes Research Foundation)

Prevention of inhibitors by oral delivery of coagulation factors (Bayer)

Mechanistic studies on immunity or tolerance using antigens/auto-antigens (funded by the National Institutes of Health)




Lab personnel:

Dr. Henry Daniell, Professor and director of Translational Research

Dr. Jin Su, senior scientist

Dr. Kwang-Chul Kwon, senior pos-doctoral fellow

Dr. Man Zhou, post-doctoral fellow

Dr. Hui-Ting Chan, post-doctoral fellow

Mr. Mark Yarmarkovich, CAMB doctoral student

Selected Publications

Kohli Neha, Westerveld Donevan R, Ayache Alexandra C, Verma Amrisha, Shil Pollob, Prasad Tuhina, Zhu Ping, Chan Sic L, Li Qiuhong, Daniell Henry: Oral delivery of bioencapsulated proteins across blood-brain and blood-retinal barriers. Molecular therapy : the journal of the American Society of Gene Therapy 22(3): 535-46, Mar 2014.

Clarke Jihong Liu, Waheed Mohammad Tahir, Lössl Andreas G, Martinussen Inger, Daniell Henry: How can plant genetic engineering contribute to cost-effective fish vaccine development for promoting sustainable aquaculture? Plant molecular biology 83(1-2): 33-40, Sep 2013.

Kwon Kwang-Chul, Verma Dheeraj, Singh Nameirakpam D, Herzog Roland, Daniell Henry: Oral delivery of human biopharmaceuticals, autoantigens and vaccine antigens bioencapsulated in plant cells. Advanced drug delivery reviews 65(6): 782-99, Jun 2013.

Wang Xiaomei, Sherman Alexandra, Liao Gongxian, Leong Kam W, Daniell Henry, Terhorst Cox, Herzog Roland W: Mechanism of oral tolerance induction to therapeutic proteins. Advanced drug delivery reviews 65(6): 759-73, Jun 2013.

Kwon Kwang-Chul, Nityanandam Ramya, New James S, Daniell Henry: Oral delivery of bioencapsulated exendin-4 expressed in chloroplasts lowers blood glucose level in mice and stimulates insulin secretion in beta-TC6 cells. Plant biotechnology journal 11(1): 77-86, Jan 2013.

Lakshmi Priya Saikumar, Verma Dheeraj, Yang Xiangdong, Lloyd Bethany, Daniell Henry: Low cost tuberculosis vaccine antigens in capsules: expression in chloroplasts, bio-encapsulation, stability and functional evaluation in vitro. PloS one 8(1): e54708, 2013.

Kwon Kwang-Chul, Verma Dheeraj, Jin Shuangxia, Singh Nameirakpam D, Daniell Henry: Release of proteins from intact chloroplasts induced by reactive oxygen species during biotic and abiotic stress. PloS one 8(6): e67106, 2013.

Verma Dheeraj, Jin Shuangxia, Kanagaraj Anderson, Singh Nameirakpam D, Daniel Jaiyanth, Kolattukudy Pappachan E, Miller Michael, Daniell Henry: Expression of fungal cutinase and swollenin in tobacco chloroplasts reveals novel enzyme functions and/or substrates. PloS one 8(2): e57187, 2013.

Wang Ying, Ding Jun, Daniell Henry, Hu Haiyan, Li Xiaoman: Motif analysis unveils the possible co-regulation of chloroplast genes and nuclear genes encoding chloroplast proteins. Plant molecular biology 80(2): 177-87, Sep 2012.

Nayak Sushrusha, Sivakumar Ramya, Cao Ou, Daniell Henry, Byrne Barry J, Herzog Roland W: Mapping the T helper cell response to acid α-glucosidase in Pompe mice. Molecular genetics and metabolism 106(2): 189-95, Jun 2012.

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Last updated: 05/13/2014
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