Henry Daniell, Ph.D.

faculty photo
Professor of Biochemistry
Department: Biochemistry
Graduate Group Affiliations

Contact information
547 Levy, 240 south 40th Street
Philadelphia, PA 19104-6030
Office: 1-215-746-2563
Fax: 1-215-898-3695
Education:
BS (Botany/Chemistry)
University of Madras, 1969.
MS (Botany)
University of Madras, 1971.
PhD (Biochemistry)
Madurai Kamaraj University, 1980.
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Description of Research Expertise

Research Interests: Oral delivery of human therapeutic proteins bio-encapsulated within plant cells to confer protection against inherited or infectious diseases

Key Words: Oral delivery, bioencapsulation in plant cells, vaccine antigens, autoimmune disorders, infectious or inherited diseases


In an emerging new concept, freeze-dried plant cells (lettuce) expressing biopharmaceuticals (fused with transmucosal carriers) are protected in the stomach from acids/enzymes but are released to the circulatory system when plant cell walls are digested by microbes that colonize the gut. Therapeutic proteins in lyophilized plant cells (in capsules) are stable >15 months at room temperature and maintain their folding, disulfide bonds, assembly and functionality. Furthermore, lyophilization process increases the antigen concentration up to 25-fold and eliminates microbes in harvested leaf materials.

Oral delivery of antigens or auto-antigens bio-encapsulated in plant cells:Several vaccine antigens (against Dengue, polio, malaria, tuberculosis, cholera, anthrax, plague, etc) or autoantigens (diabetes, hemophilia, etc) have been expressed in lettuce chloroplasts. Vaccine antigens bio-encapsulated in plant cells upon oral delivery after priming, conferred both mucosal and systemic immunity and protection against bacterial, viral or protozoan pathogens or toxin challenge. Oral delivery of autoantigens was effective in delaying the onset of type 1 diabetes or inhibitory antibodies/complications after injection of blood clotting factors in hemophiliaA/B, by developing tolerance.


Oral delivery of functional proteins: Oral delivery of exendin-4 expressed in plant cells regulated blood glucose levels similar to injections by stimulating insulin secretion and a 5,000 fold excess dose didn’t cause hypoglycemia because insulinotropism of exendin-4 is glucose dependent. Oral delivery of angiotensin converting enzyme 2 (ACE2) or angiotensin-(1-7) attenuated pulmonary hypertension and associated cardiac dysfunction. Amyloid plaques were reduced in vivo by 70% in hippocampus and cortex brain regions when fed with myelin basic protein and reduced Aβ42 accumulation in retinae and prevented loss of retinal ganglion cells in 3xTg Alzheimer’s disease mice.

Therefore, this new platform should offer a low cost alternative to deliver human therapeutic proteins to combat infectious or inherited diseases by eliminating inactivated pathogens, expensive purification, cold storage/transportation and sterile injections.


Rotation projects:

Low cost oral polio booster vaccine (funded by the Bill and Melinda Gates Foundation)

Oral immune modulatory therapy using antigens bio-encapsulated in plant cells (funded by the National Institutes of Health)

Oral therapy for hemophilia A (funded by the National Institutes of Health)

Preventing type 1diabetes by oral delivery of plant-derived auto-antigens (funded by the Juvenile Diabetes Research Foundation)

Prevention of inhibitors by oral delivery of coagulation factors (Bayer)

Mechanistic studies on immunity or tolerance using antigens/auto-antigens (funded by the National Institutes of Health)




Lab personnel:

Dr. Henry Daniell, Professor and director of Translational Research

Dr. Jin Su, senior scientist

Dr. Kwang-Chul Kwon, senior pos-doctoral fellow

Dr. Man Zhou, post-doctoral fellow

Dr. Hui-Ting Chan, post-doctoral fellow

Mr. Mark Yarmarkovich, CAMB doctoral student

Selected Publications

Kwon Kwang-Chul, Verma Dheeraj, Singh Nameirakpam D, Herzog Roland, Daniell Henry: Oral delivery of human biopharmaceuticals, autoantigens and vaccine antigens bioencapsulated in plant cells. Advanced drug delivery reviews 65(6): 782-99, Jun 2013.

Kwon Kwang-Chul, Nityanandam Ramya, New James S, Daniell Henry: Oral delivery of bioencapsulated exendin-4 expressed in chloroplasts lowers blood glucose level in mice and stimulates insulin secretion in beta-TC6 cells. Plant biotechnology journal 11(1): 77-86, Jan 2013.

Lakshmi Priya Saikumar, Verma Dheeraj, Yang Xiangdong, Lloyd Bethany, Daniell Henry: Low cost tuberculosis vaccine antigens in capsules: expression in chloroplasts, bio-encapsulation, stability and functional evaluation in vitro. PloS one 8(1): e54708, 2013.

Boyhan Diane, Daniell Henry: Low-cost production of proinsulin in tobacco and lettuce chloroplasts for injectable or oral delivery of functional insulin and C-peptide. Plant biotechnology journal 9(5): 585-98, Jun 2011.

Lee Seung-Bum, Li Baichuan, Jin Shuangxia, Daniell Henry: Expression and characterization of antimicrobial peptides Retrocyclin-101 and Protegrin-1 in chloroplasts to control viral and bacterial infections. Plant biotechnology journal 9(1): 100-15, Jan 2011.

Verma Dheeraj, Moghimi Babak, LoDuca Paul A, Singh Harminder D, Hoffman Brad E, Herzog Roland W, Daniell Henry: Oral delivery of bioencapsulated coagulation factor IX prevents inhibitor formation and fatal anaphylaxis in hemophilia B mice. Proceedings of the National Academy of Sciences of the United States of America 107(15): 7101-6, Apr 2010.

Davoodi-Semiromi Abdoreza, Schreiber Melissa, Nalapalli Samson, Verma Dheeraj, Singh Nameirakpam D, Banks Robert K, Chakrabarti Debopam, Daniell Henry: Chloroplast-derived vaccine antigens confer dual immunity against cholera and malaria by oral or injectable delivery. Plant biotechnology journal 8(2): 223-42, Feb 2010.

Arlen Philip A, Singleton Michael, Adamovicz Jeffrey J, Ding Yi, Davoodi-Semiromi Abdolreza, Daniell Henry: Effective plague vaccination via oral delivery of plant cells expressing F1-V antigens in chloroplasts. Infection and immunity 76(8): 3640-50, Aug 2008.

Arlen Philip A, Falconer Regina, Cherukumilli Sri, Cole Amy, Cole Alexander M, Oishi Karen K, Daniell Henry: Field production and functional evaluation of chloroplast-derived interferon-alpha2b. Plant biotechnology journal 5(4): 511-25, Jul 2007.

Ruhlman Tracey, Ahangari Raheleh, Devine Andrew, Samsam Mohtahsem, Daniell Henry: Expression of cholera toxin B-proinsulin fusion protein in lettuce and tobacco chloroplasts--oral administration protects against development of insulitis in non-obese diabetic mice. Plant biotechnology journal 5(4): 495-510, Jul 2007.

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Last updated: 03/22/2014
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