Ana Lia Obaid, Ph.D.

faculty photo
Research Associate Professor of Neuroscience
Department: Neuroscience
Graduate Group Affiliations

Contact information
Department of Neuroscience
University of Pennsylvania
234 Stemmler Hall
Philadelphia, PA 19104-6074
Office: (215) 898-6114
Fax: (215) 746-2758
Education:
M.S. (Biochemistry)
University of Rosario, Argentina, 1969.
Ph.D. (Biochemistry)
University of Buenos Aires, Argentina, 1974.
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Description of Research Expertise

RESEARCH INTERESTS
Optical measurements of electrical activity; excitation-secretion coupling; simple nervous systems in vitro; enteric nervous system

KEY WORDS:
Voltage-sensitive dyes; light scattering; excitation-secretion coupling; enteric nervous system; submucous plexus

RESEARCH TECHNIQUES
Multiple-site optical recording of membrane potential (absorption and fluorescence) and calcium transients with milli-second time resolution; intrinsic optical signals; primary cultures of identified invertebrate neurons; optical recording of electrical activity in mammalian networks, with single cell resolution.


RESEARCH SUMMARY
I am interested in the application of optical techniques to a variety of physiological problems. These include excitation-secretion coupling, and studies of functional connectivity in small neuronal ensembles. Most of my recent work has employed a system for Multiple Site Optical Recording of Transmembrane Voltage (MSORTV), which, in conjunction with voltage-sensitive dyes, permits one to monitor electrical events in cells or parts of cells not easily accessible with conventional electrophysiological techniques. For example, we have been able to measure the electrical activity (using dyes) and the resultant secretory events (using light scattering) at the nerve terminals of the vertebrate neurohypophysis. We have also used optical techniques to study patterns of electrical activity in simple nervous systems, including small ensembles of identified neurons in tissue culture, and rings of submucosal ganglia of the guinea-pig small intestine.

Selected Publications

Salzberg, B.M., Kosterin, P.V., Muschol, M., Obaid, A.L., Rumyantsev, S.L., Bilenko, Yu., and Shur, M.S. : An ultra-stable non-coherent light source for optical measurements in neuroscience and cell physiology. J. Neurosci. Methods 141(1): 165-169, Jan 2005.

Obaid, A.L., L.M. Loew, J.P. Wuskell, and B.M. Salzberg: Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis. J. Neurosci. Methods 134: 179-90, Apr 2004.

Obaid, A.L., J.F. Cooper, and J. Lindstrom: Nicotinic receptors in guinea pig gut: Validation of monoclonal antibody specificity. Neurosci. Abstr. 31: A374.11, 2001.

Salzberg, B.M., M. Muschol, S.D. Kraner, and A.L. Obaid: Localization of calcium release channels to secretory granules in terminals of the mouse neurohypophysis Biophys. J. 78: 260a, 2000.

Obaid, A.L., J. Lindstrom, and B.M. Salzberg: Cholinergic Transmission in the enteric nervous system: Optical studies of network behavior and immunocytochemical localization of AChR subtypes Proceedings of the International Conference on "Optical Approaches to Physiological Functions", Tokyo, Japan 2000.

Salzberg, B.M., M. Muschol, and A.L. Obaid: "Intrinsic and extrinsic optical signals from mammalian nerve terminals". Proceedings of the International Conference on "Optical Approaches to Physiological Functions", Tokyo, Japan 2000.

Obaid AL., Koyano T., Lindstrom J., Sakai T., Salzberg BM.: Spatiotemporal patterns of activity in an intact mammalian network with single-cell resolution: optical studies of nicotinic activity in an enteric plexus. J. Neurosci. 19(8): 3073-3093, Apr 1999.

Salzberg, B.M., M. Muschol, and A.L. Obaid: "Rapid changes in light scattering associated with secretion from peptidergic nerve terminals". Imaging: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1999.

Salzberg, B.M., M. Muschol, S.D. Kraner, and A.L. Obaid: "Calcium release channels are localized to neurosecretory granules in terminals of the mouse neurohypophysis". J. Gen. Physiol. 114: 9a, 1999.

Obaid, A.L., G.B. Wells, A. Kuryatov, and J. Lindstrom: "nAChR subunits that mediate nicotinic activity in the enteric nervous system". Neurosci. Abstr. 25: 1490, 1999.

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Last updated: 12/09/2014
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