Department of Microbiology

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Faculty Profile

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Michael R. Betts, PhD

Associate Professor of Microbiology
Department: Microbiology

Contact information
402C Johnson Pavilion
3610 Hamilton Walk
Philadelphia, PA 19104
Office: 215-573-2773
Fax: 215-573-4446
Graduate Group Affiliations
Education:
BS (Zoology)
University of Maryland, 1990.
PhD (Microbiology and Immunology)
University of North Carolina, 1998.
Permanent link
 
> Perelman School of Medicine   > Faculty   > Details

Description of Research Expertise

Research Interests
Human immune responses; Viral Immunology; HIV immunopathogenesis; Vaccine-induced immune responses.

Key words: HIV; T cell; Immune Response

Description of Research
My laboratory studies human T lymphocyte function in order to understand the role of these cells in controlling or eliminating viral pathogens and providing protection from infection. Our primary interest is in determining how and if the human CD8+ T cell response to HIV controls viral replication. We also study the immune response against a variety of other human pathogens, including Epstein-Barr virus, cytomegalovirus, influenza, and vaccinia virus. Importantly, the techniques we utilize can be applied to the study of the cell-mediated immune response against any human pathogen, including emerging pathogens and bioterrorism agents. We are also very interested in characterizing the human T cell response to various vaccine regimens against a variety of human pathogens designed to generate cell-mediated immunity in order to understand the underlying principles of vaccine-mediated immune protection.

Human T lymphocytes have numerous functions, including the ability to produce various cytokines and chemokines, as well as mediate cell death through perforin- or fas-mediated cytotoxicity. Our research utilizes the most cutting edge techniques to measure human T lymphocyte responses through the use of polychromatic flow cytometry. This technique allows for the simultaneous examination of up to 18 separate parameters on lymphocytes. By measuring multiple T cell functions simultaneously, we can characterize the complexity of the CD4+ and CD8+ T cell response to HIV, EBV, CMV, Flu, and vaccinia. Not surprisingly, the T cell responses to these different viruses are quite variable; however, common response patterns do exist, and the importance of these patterns in the control of viral replication is the subject of future studies.

The current direction within the lab is to determine the underlying mechanisms that control the cell fate and functional characteristics of HIV-specific T and B cells in the context of HIV infection and disease progression. Current studies in the lab in this regard are listed below with Laboratory personnel:

Lab personnel:
Morgan Reuter-Moslow, Postdoctoral Fellow. HIV-specific T cell immunity. T cell function in human lymph nodes; CD200-CD200R pathway interactions in human T lymphocytes.

Laura McLane, Postdoctoral Fellow. Expression, localization, and biochemical properties of the transcription factors T-bet and Eomesodermin within human T lymphocytes.

Korey Demers, Graduate Student-CAMB MVP. Role of T-bet and Eomesodermin transcription patterns in CD8+ T cell mediated control of HIV infection during acute and chronic infection.

Carolina Pombo Martinez, Graduate Student-CAMB MVP. Expression patterns of inhibitory receptors in human T cells in peripheral blood and lymph node in the context of HIV infection.

Irene Bukh, Graduate Student-VMD/PHD, CAMB MVP. Vaccine induced immune activation in rhesus macaques and SIV infection susceptibility.

Jamie Knox, Graduate Student- CAMB MVP. B cell dynamics in HIV infection. T-bet expression and function in B cell subsets in humans.

Emily Roberts, Graduate Student- IGG. Immune activation and SIV-specific CD4+ and CD8+ T cell response development in acute SIV infection.
Jay Gardner, Research Specialist

Selected Publications

Riou C, Ganusov VV, Campion S, Mlotshwa M, Liu MK, Whale VE, Goonetilleke N, Borrow P, Ferrari G, Betts MR, Haynes BF, McMichael AJ, Gray CM: Distinct kinetics of Gag-specific CD4+ and CD8+ T cell responses during acute HIV-1 infection. J. Immunol. Jan 2012.

van Montfrans JM, Hoepelman AL, Otto S, van Gijn M, van de Corput L, de Weger RA, Monaco-Shawver L, Banerjee PP, Sanders EA, Jol-van der Zidje CM, Betts MR, Orange JS, Bloem AC, Tesselaar K.: CD27 deficiency is associated with combined immunodeficiency and persistent symptomatic EBV viremia. J. Allergy Clin. Immunol. Epub Dec 2011.

Hersperger Adam R, Migueles Stephen A, Betts Michael R, Connors Mark: Qualitative features of the HIV-specific CD8+ T-cell response associated with immunologic control. Current opinion in HIV and AIDS 6(3): 169-73, May 2011.

Haney D, Quigley MF, Asher TE, Ambrozak DR, Gostick E, Price DA, Douek DC, Betts MR: Isolation of viable anteing-specific CD8+ T-cells based on membrane-bound tumor necrosis factor (TNF)-a expression. J. Imm. Methods. Epub, April 2011.

Hersperger Adam R, Martin Jeffrey N, Shin Lucy Y, Sheth Prameet M, Kovacs Colin M, Cosma Gabriela L, Makedonas George, Pereyra Florencia, Walker Bruce D, Kaul Rupert, Deeks Steven G, Betts Michael R: Increased HIV-specific CD8+ T-cell cytotoxic potential in HIV elite controllers is associated with T-bet expression. Blood 117(14): 3799-808, Apr 2011.

E. Fenniri L, Toossi Z, Aung H, El Iraki G, Bourkkadi J, Benamor J, Laskri A, Berrada N, Benjouad A, Mayanja-Kizza H, Betts MR, El Aouad R, Canaday DH: Polyfunctional M. tuberculosis-specific effector memory CD4+ T cells at sites of pleural TB. Tuberculosis. Epup, April 2011.

Hersperger AR, Martin JN, Shin LY, Sheth PM, Kovacs CM, Cosma GL, Makedonas G, Pereyra F, Walker BD< Kaul R, Deeks SG, Betts MR: Increased HIV-specific CD8+ T-cell cytoxic potential in HIV elite controllers is associated with T-bet expression. Blood 117: 3799 - 3808, April 2011 Notes: Evaluated by Faculty of 1000 Medicine.

Ferrari G, Korber B, Goonetilleke N, Liu MKP, Turnbull EL, Salazar-Gonzalez JF, Hawkins N, Self S, Watson S, Betts MR, Gay C, McGhee K, Pellegrino P, Williams I, Tomaras GD, Haynes BF, Gray CM, Borrow P, Roederer M., McMichael AJ, Weinhold KJ: Relationship between functional profile of HIV-1 specific CD8 T-cells and epitope variability with the selection of escape mutants in acute HIV-1 infection. PLoS Pathogens. Epub, Feb 2011.

Lasaro Marcio O, Haut Larissa H, Zhou Xiangyang, Xiang Zhiquan, Zhou Dongming, Li Yan, Giles-Davis Wynetta, Li Hua, Engram Jessica C, Dimenna Lauren J, Bian Ang, Sazanovich Marina, Parzych Elizabeth M, Kurupati Raj, Small Juliana C, Wu Te-Lang, Leskowitz Rachel M, Klatt Nicole R, Brenchley Jason M, Garber David A, Lewis Mark, Ratcliffe Sarah J, Betts Michael R, Silvestri Guido, Ertl Hildegund C: Vaccine-induced T cells provide partial protection against high-dose rectal SIVmac239 challenge of rhesus macaques. Molecular therapy : the journal of the American Society of Gene Therapy 19(2): 417-26, Feb 2011.

Makedonas George, Betts Michael R: Living in a house of cards: re-evaluating CD8+ T-cell immune correlates against HIV. Immunological reviews 239(1): 109-24, Jan 2011.

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Last updated: 01/31/2014
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