Description of Research
The human genome contains ~20,000 protein-coding genes representing less than 2% of the total genome, whereas up to 70% of the human genome is transcribed to RNA, yielding many thousands of non-coding RNAs. The recent discovery of non-coding RNA genes has dramatically altered our understanding on cancer. Long non-coding RNAs (lncRNAs) are operationally defined as RNA genes larger than 200 nucleotide that do not appear to have protein coding potential. micoRNAs (miRNAs) are endogenous ~18-25 nucleotide non-coding small RNAs which regulate gene expression in a sequence-specific manner via the degradation of target mRNAs or the inhibition of protein translation. The Zhang laboratory is interested in molecular mechanisms of non-coding RNAs (microRNA and lncRNA) in tumorigenesis, and the potential application of non-coding RNA in treatment for breast and ovarian cancer. High-throughput profiling technologies (microarray and deep-sequencing) and whole-genome wide miRNA/siRNA screening are used in the laboratory.
OncoGenome and EpiGenome in ovarian and breast cancers
microRNAs/long non-coding RNAs in cancer
microRNA-based therapeutic and diagnostic strategies in cancer
microRNAs in cancer stem cells and embryonic stem cells
Zhang Youyou, He Qun, Hu Zhongyi, Feng Yi, Fan Lingling, Tang Zhaoqing, Yuan Jiao, Shan Weiwei, Li Chunsheng, Hu Xiaowen, Tanyi Janos L, Fan Yi, Huang Qihong, Montone Kathleen, Dang Chi V, Zhang Lin: Long noncoding RNA LINP1 regulates repair of DNA double-strand breaks in triple-negative breast cancer. Nature structural & molecular biology Apr 2016.
Yan Xiaohui, Hu Zhongyi, Feng Yi, Hu Xiaowen, Yuan Jiao, Zhao Sihai D, Zhang Youyou, Yang Lu, Shan Weiwei, He Qun, Fan Lingling, Kandalaft Lana E, Tanyi Janos L, Li Chunsheng, Yuan Chao-Xing, Zhang Dongmei, Yuan Huiqing, Hua Keqin, Lu Yiling, Katsaros Dionyssios, Huang Qihong, Montone Kathleen, Fan Yi, Coukos George, Boyd Jeff, Sood Anil K, Rebbeck Timothy, Mills Gordon B, Dang Chi V, Zhang Lin: Comprehensive Genomic Characterization of Long Non-coding RNAs across Human Cancers. Cancer Cell 28(4): 529-40, Oct 2015.
Hu X, Feng Y, Zhang D, Zhao SD, Hu Z, Greshock J, Zhang Y, Yang L, Zhong X, Wang L-P, Jean S, Li C, Huang Q, Katsaros D, Montone KT, Tanyi JL, Lu Y, Boyd J, Nathanson KL, Li H, Mills GB, Zhang L: A Functional Genomic Approach Identifies FAL1 as an Oncogenic Long Noncoding RNA that Associates with BMI1 and Represses p21 Expression in Cancer. Cancer Cell 26(3): 344-57, Sep 2014.
Li N, Kaur S, Greshock J, Lassus H, Zhong X, Wang Y, Leminen A, Shao X, Hu X, Liang S, Katsaros D, Huang Q, Bützow R, Weber BL, Coukos G, Zhang L: A combined array-based comparative genomic hybridization and functional library screening approach identifies mir-30d as an oncomir in cancer. Cancer Res 72(1): 154-64, Jan 2012.
Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang L-P, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G: Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature 475(7355): 226-30, Jul 2011.
Yang X, Lin X, Zhong X, Kaur S, Li N, Liang S, Lassus H, Wang L, Katsaros D, Montone K, Zhao X, Zhang Y, Bützow R, Coukos G, Zhang L: Double-negative feedback loop between reprogramming factor LIN28 and microRNA let-7 regulates aldehyde dehydrogenase 1-positive cancer stem cells. Cancer Res 70(22): 9463-72, Nov 2010.
Gumireddy K, Li A, Gimotty PA, Klein-Szanto AJ, Showe LC, Katsaros D, Coukos G, Zhang L, Huang Q: KLF17 is a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Nature Cell Biol 11(11): 1297-304, Nov 2009.
Yang N, Kaur S, Volinia S, Greshock J, Lassus H, Hasegawa K, Liang S, Leminen A, Deng S, Smith L, Johnstone CN, Chen X-M, Liu C-G, Huang Q, Katsaros D, Calin GA, Weber BL, Bützow R, Croce CM, Coukos G, Zhang L: MicroRNA microarray identifies Let-7i as a novel biomarker and therapeutic target in human epithelial ovarian cancer. Cancer Res 68(24): 10307-14, Dec 2008.
Zhang L, Huang J, Yang N, Greshock J, Megraw MS, Giannakakis A, Liang S, Naylor TL, Barchetti A, Ward MR, Yao G, Medina A, O'brien-Jenkins A, Katsaros D, Hatzigeorgiou A, Gimotty PA, Weber BL, Coukos G: microRNAs exhibit high frequency genomic alterations in human cancer. Proceed Nat Acad Sciences of USA 103(24): 9136-41, Jun 2006.
Zhang L, Conejo-Garcia JR, Katsaros D, Gimotty PA, Massobrio M, Regnani G, Makrigiannakis A, Gray H, Schlienger K, Liebman M N, Rubin SC, Coukos G: Intratumoral T cells, recurrence, and survival in epithelial ovarian cancer. New England Journal of Medicine 348(3): 203-13, Jan 2003.
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Last updated: 04/28/2016
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