Services » Embryo Cryopreservation
Freezing mouse embryos is a convenient way to preserve in important mouse line for possible future use or to protect this line against loss due to infection, lab accident, etc. Embryo freezing could also result in substantial reduction in housing cost and cage use.
A set of five to seven young male mice will be needed to generate enough embryos for each freezing set. The male founders will be subjected to a waiting period in the containment area, as required by ULAR, prior to admission into the barrier colony.
A minimum of 300 healthy embryos will be frozen per transgenic/knockout line that is hemizygous for the transgene. In the case where the mouse line is homozygous for the transgene, 150 embryos will suffice. Several weeks after the completion of the freezing process, a few embryo samples will be removed and processed through thawing and monitoring development to assess their viability and ability to develop to term. The facility will now store the frozen samples and maintain the records of your cryopreserved lines.
Alternate Services Available from the TCMF Core for Cryopreservation and Re-derivation of Mouse Lines
High efficiency of embryo freezing will depend primarily on the reproductive level of the breeders since low mating frequency and low percentage of fertilization will lead to a prolonged time-consuming process.
The investigator should contact Jean Richa (215/573-3023) to discuss the plans for the service. These would include the availability of the males, the possibility of admitting the animals into the barrier facility, the number of mouse lines involved, and the service fees.