Services » Sperm Cryopreservation
This service provides an alternate procedure to preserve important mouse lines that either fail to generate embryos for cryopreservation, or when the breeder males are not located within the barrier Facility. It offers similar benefits to those of embryo cryopreservation by protecting the mouse lines from losses and by reducing the housing cost and cage space.
For this service we recommend a one-time harvest of embryos to be frozen prior to sacrificing the males for cryopreservation.
A set of 5-7 young male mice will be subjected to a waiting period in the containment area, as required by ULAR, prior to admission into the barrier colony. Once admitted, the animals will be mated with females to generate embryos to be frozen. Then 3 males will be sacrificed and the sperm collected and frozen.
Alternate Services Available from the TCMF Core for Cryopreservation and Re-derivation of Mouse Lines
It is highly recommended that young and productive males are used for this process, so that the future line re-derivation process through IVF would be successful.
The investigator should contact Jean Richa (215/573-3023) to discuss the plans for the service. These would include the availability of the males, the possibility of admitting the animals into the barrier facility, the number of mouse lines involved, and the service fees.