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Department of Pharmacology
David R. Manning, Ph.D.

Education:

B.A. (Biology)	University of California at San Diego
Ph.D. (Physiology/Pharmacology)	University of California at San Diego

Research Summary:
The major focus of the laboratory is the process by which the binding of hormones to cell-surface receptors is translated into the regulation of target enzymes and ion channels. The laboratory is specifically interested in pathways defined by GTP-binding regulatory proteins (G proteins). Current studies include mapping linkages among receptors and G proteins in intact cells, defining post-translational modifications of G protein subunits that influence targeting and protein-protein interactions, exploring the roles of novel G proteins, and defining connections between G proteins and various transcription factors. The context of greatest interest is cell proliferation and differentiation. Pathways receiving the greatest attention currently are those used by lysophosphatidic acid, sphingosine 1-phosphate, and thromboxane A₂.

Selected Key Publications:

Siehler S, Wang Y, Fan X, Windh RT & Manning DR: Sphingosine 1-phosphate activates nuclear factor- B through Edg receptors: Activation through Edg-3 and Edg-5, but not Edg-1, in human embryonic kidney 293 cells. J Biol Chem 276: 48733-48739, 2001.

Manning DR: Measures of efficacy using G proteins as endpoints: Differential engagement of G proteins through single receptors. Mol Pharmacol 62: 451-452, 2002.

Manning DR: Evidence mounts for receptor-independent activation of heterotrimeric G proteins normally in vivo: positioning of the mitotic spindle in C. elegans. Sci STKE pe35, 2003.

Riobo NA & Manning DR: Receptors coupled to heterotrimeric G proteins of the G₁₂ family. Trends Pharmacol Sci 26: 146-154, 2005.

Zhang L, DiLizio C, Kim D, Smith EM & Manning DR: The G₁₂ family of G proteins as a reporter of thromboxane A₂ activity. Mol Pharmacol 69: 1433-1440, 2006.

Riobo NA, Saucy B, DiLizio C & Manning DR: Activation of heterotrimeric G proteins by Smoothened. Proc. Natl. Acad. Sci. (USA) 103: 12607-12612, 2006.

Awards, Honors, Membership in Honorary Societies:

•	Dean's Award for Excellence in Graduate Student Training (1994)
•	Lindback Award for DistinguishedTeaching (1999)
•	Chairman, Cell Biology and Physiology-2 NIH Study Section (1994-99)
•	Chairman, Division of Molecular Biology, ASPET (2003-04)
•	GGPS Annual Faculty Award (2007)

Editorial Board Memberships:

•	The Journal of Biological Chemistry (1995-2006)
•	Molecular Pharmacology (2002-present)

BIOL448, Principles of Drug Action:

•	Syllabus
•	Schedule

Laboratory Personnel:

Cherisse Dilizio Research Associate cdilizio@mail.med.upenn.edu

Li Zhang Post-doctoral Fellow lzhang@mail.med.upenn.edu

Andrew Douglas Postdoctoral Fellow adougl@mail.med.upenn.edu

Lab Address:
Department of Pharmacology
116 John Morgan Building
University of Pennsylvania School of Medicine
3620 Hamilton Walk
Philadelphia, PA 19104-6084

Lab Telephone:   215-898-9769    Lab Fax:   215-573-2236