Faculty

Matthew C Good, Ph.D.

faculty photo
Assistant Professor of Cell and Developmental Biology
Department: Cell and Developmental Biology
Graduate Group Affiliations

Contact information
1151 BRB II/III
421 Curie Boulevard
Philadelphia, PA 19104
Office: 215-573-1099
Lab: 215-573-6805
Education:
BA (Biochemistry)
University of California, Berkeley, 2003.
PhD (Biochemistry)
University of California, San Francisco, 2010.
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Description of Research Expertise

RESEARCH INTERESTS
* Adaptability of intracellular structures to variation in cell size and shape
* Temporal and spatial regulation of zygotic genome activation
* Self-assembly of intrinsically disordered proteins
* Phase separation and membraneless organelles
* Subcellular organization of intracellular signaling pathways
* Synthetic cells and protocells, synthetic organelles
* Optochemical protein dimerization

KEY WORDS
cell size, intracellular size scaling, size regulation, embryogenesis, zygotic genome activation, transcriptional imaging, Xenopus, zebrafish, optochemical dimerization, split enzymes, cell-free systems, encapsulation, microfluidics, emulsions, synthetic cells, IDPs, membraneless organelles

DESCRIPTION OF RESEARCH

Our lab is interested: 1) cell size control and the downstream consequences of size-dysregulation, and 2) coordinated protein assembly into mesoscale structures, including membraneless organelles, the mitotic spindle and signaling complexes. To investigate the impacts of cell size on cell function, we characterize early embryo development, a period in which cells rapidly divide without growing. Additionally, we are investigating diseased cell states, such as cancer, in which proper cell size homeostasis is lost. To characterize principles that drive the self-assembly of proteins, we focus on scaling and positioning of the mitotic spindle within cleavage stage embryos and in vitro. Additionally we aim to decipher properties of disordered proteins that drive their self-assembly and phase separation into membraneless organelles.

We utilize interdisciplinary approaches to address fundamental questions in cell biology. This strategy requires that we develop new tools and strategies, including to image and sequence the nascent transcriptome during embryo development, designer proteins that self-assemble to form synthetic membraneless organelles, microfluidic approaches to encapsulate proteins and cell-free cytoplasmic mixtures inside cell-like compartments in vitro; optochemical dimerizers to control protein localization and enzymatic activity within protocells. Our model systems include: zebrafish and Xenopus cleavage-stage embryos, mammalian cell culture, and biomimetic synthetic cells. We seek a better understanding of biology through bottom-up reconstitution using minimal sets of components.

Selected Publications

Garabedian M.V., Wang W., Dabdoub J.B., Tong M., Caldwell R.M., Ahmed H., Benman W., Schuster B.S., Deiters A., and Good M.C.*: Designer Membraneless Organelles Sequester Native Factors for Modular Control of Cell Behavior. Under Revision, Nature Chemical Biology 2021.

Bermudez J.G., Deiters A., Good M.C.*: Patterning Microtubule Network Organization Reshapes Cell-Like Compartments. ACS Synthetic Biology May 2021 Notes: doi: 10.1021/acssynbio.0c00575.

Schuster B.S., Dignon G.L., Tang W.S., Kelley F.M., Ranganath A.K., Jahnke C.N., Simpkins A.G., Regy R.M., Hammer D.A., Good M.C., Mittal J. : Identifying Sequence Perturbations to an Intrinsically Disordered Protein that Determine Its Phase Separation Behavior. PNAS 117(21): 11421-11431, May 2020.

Li, S., Xia, B., Javed, B., Hasley, W.D., Melendez-Davila, A., Liu, M., Kerzner, M., Agarwal, S., Xiao, Q., Torre, P., Bermudez, J.G., Rahimi, K., Kostina, N.Y., Möller, M., Rodriguez-Emmenegger,C., Klein, M.L., Percec, V. and Good, M.C.* : Direct Visualization of Vesicle Disassembly and Reassembly Using Photocleavable Dendrimers Elucidates Cargo Release Mechanisms. ACS Nano 14: 7398-7411 May 2020.

Chen, H., Good, M.C.*: Imaging Nascent Transcription in Wholemount Vertebrate Embryos to Characterize Zygotic Genome Activation. Methods In Enzymology 638: 139-165, April 2020.

Chen H., Einstein, L., Little S., Good M.C.*: Spatiotemporal Patterning of Zygotic Genome Activation in A Model Vertebrate Embryo. Developmental Cell 49(6): 852-866, June 2019.

Torre P., Xiao Q., Buzzacchera I., Sherman S.E., Rahimi K., Kostina N.Y., Rodriguez-Emmenegger C., Möller M., Wilson C.J., Klein M.L.*, Good M.C.* and Percec V.* : Encapsulation of Hydrophobic Components in Dendrimersomes and Decoration of their Surface with Proteins and Nucleic Acids. PNAS 116(31): 15378-15385, July 2019.

Schuster B.S., Reed E.H., Parthasarathy R., Jahnke C.N., Caldwell R.M., Bermudez J.G., Ramage H., Good M.C.*, Hammer D.A.*: Controllable Protein Phase Separation and Modular Recruitment to Form Responsive, Membraneless Organelles. Nature Communications 9(1): 2985, July 2018.

Caldwell R.M., Bermudez J.G., Thai D., Aonbangkhen C., Schuster B.S., Courtney T., Deiters A., Hammer D.A., Chenoweth D.M., Good M.C.*: Optochemical Control of Protein Localization and Activity within Cell-like Compartments. Biochemistry 57(18): 2590-2596, May 2018.

Reed E.H., Schuster B.S., Good M.C., Hammer D.A: SPLIT: Stable Protein Coacervation Using a Light Induced Transition. ACS Synthetic Biology 9: 500-507, Mar 2020.

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Last updated: 06/07/2021
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