Andrew J Modzelewski, PhD

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Graduate Group Affiliations

Contact information
411 Hill Pavilion
Philadelphia, PA 19104
B.Sc. (Biochemistry and Molecular Biology)
Pennsylvania State University, 2007.
PhD (Molecular Biology and Genetics)
Cornell University, 2013.
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Description of Research Expertise

• Retrotransposon Reactivation: I hope to uncover the emerging roles these ancient parasites have now been co-opted to provide by our genomes. While preimplantation provides an excellent naturally occurring starting point for this analysis, reactivation of retrotransposons also occurs during aging and various disease states. My work hopes to understand how these domesticated elements function in a “normal” setting in order to apply these findings towards the various pathologies we are facing.

• Technology development: CRISPR-EZ and Single Cell Triple Measurements (Western/RT-PCR/DNA), I aim to provide solutions to roadblocks I've encountered in my research to the broader research community. While not the focus of my primary research goals, I genuinely enjoy creating and sharing solutions so that frustrated and modestly funded labs have a better chance at answering their passionate biological questions. There is a tool for everything, we just need to make them sometimes.

• Collectively, I want to reveal more about the role of non-coding RNAs (miRNA, siRNA and Retrotransposons) and how defects in these normal biological processes affect human and animal reproduction. I have also generated some of the tools that will not only help research these biological processes, but also have already been adapted to direct and immediate practical application in research and animal production.

Selected Publications

Rosas-Canyelles, E., Modzelewski, A. J., Geldert, A., He, L., Herr, A. E.: Multimodal detection of protein isoforms and nucleic acids from mouse pre-implantation embryos. Nature Protocols 16(2): 1062-1088, 2021.

Modzelewski, A. J., Shao, W., Chen, J., Lee, A., Qi, X., Noon, M., Tjokro, K., Sales, G., Biton, A., Anand, A., Speed, T. P., Xuan, Z., Wang, T., Risso, D., He, L.: A mouse-specific retrotransposon drives a conserved Cdk2ap1 isoform essential for development. Cell 184(22): 5541-5558.e22, 2021.

Tsitsiklis, A., Bangs, D. J., Lutes, L. K., Chan, S. W., Geiger, K. M., Modzelewski, A. J., Labarta-Bajo, L., Wang, Y., Zuniga, E. I., Dai, S., Robey, E. A.: An unusual MHC molecule generates protective CD8+ T cell responses to chronic infection. Frontiers in Immunology 11: 1464, 2020.

Rosas-Canyelles, E., Modzelewski, A. J., Geldert, A., He, L., Herr, A. E.: Assessing heterogeneity among single embryos and single blastomeres using open microfluidic design. Sci Adv 6(17): eaay1751, 2020.

Modzelewski, A. J., Chen, S., Willis, B. J., Lloyd, K. C. K., Wood, J. A., He, L.: Efficient mouse genome engineering by CRISPR-EZ technology. Nature Protocols 13(6): 1253-1274, 2018.

Hilz, S., Fogarty, E. A., Modzelewski, A. J., Cohen, P. E., Grimson, A.: Transcriptome profiling of the developing male germ line identifies the miR-29 family as a global regulator during meiosis. RNA Biology 14(2): 219-235, 2017.

Sun, X., Brieno-Enriquez, M. A., Cornelius, A., Modzelewski, A. J., Maley, T. T., Campbell-Peterson, K. M., Holloway, J. K., Cohen, P. E.: FancJ (Brip1) loss-of-function allele results in spermatogonial cell depletion during embryogenesis and altered processing of crossover sites during meiotic prophase I in mice. Chromosoma 125(2): 237-52, 2016.

Hilz, S., Modzelewski, A. J., Cohen, P. E., Grimson, A.: The roles of microRNAs and siRNAs in mammalian spermatogenesis. Development 143(17): 3061-73, 2016.

Chen, S., Lee, B., Lee, A. Y., Modzelewski, A. J., He, L.: Highly efficient mouse genome editing by CRISPR ribonucleoprotein electroporation of zygotes. Journal of Biological Chemistry 291(28): 14457-67, 2016.

Modzelewski, A. J., Hilz, S., Crate, E. A., Schweidenback, C. T., Fogarty, E. A., Grenier, J. K., Freire, R., Cohen, P. E., Grimson, A.: Dgcr8 and Dicer are essential for sex chromosome integrity during meiosis in males. Journal of Cell Science 128(12): 2314-27, 2015.

Zhang, J., Bonasio, R., Strino, F., Kluger, Y., Holloway, J. K., Modzelewski, A. J., Cohen, P. E., Reinberg, D.: SFMBT1 functions with LSD1 to regulate expression of canonical histone genes and chromatin-related factors. Genes & Development 27(7): 749-66, 2013.

Modzelewski, A. J., Holmes, R. J., Hilz, S., Grimson, A., Cohen, P. E.: AGO4 regulates entry into meiosis and influences silencing of sex chromosomes in the male mouse germline. Developmental Cell 23(2): 251-64, 2012.

Lyndaker, A. M., Modzelewski, A. J., Welsh, I. C.: Reproductive and developmental genomics retreat at Cornell University, 2012. Molecular Reproduction and Development 79(9): 588-91, 2012.

Holloway, J. K., Mohan, S., Balmus, G., Sun, X., Modzelewski, A., Borst, P. L., Freire, R., Weiss, R. S., Cohen, P. E.: Mammalian BTBD12 (SLX4) protects against genomic instability during mammalian spermatogenesis. PLoS Genetics 7(6): e1002094, 2011.

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Last updated: 07/27/2022
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