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Erika L.F. Holzbaur, Ph.D.
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William Maul Measey Professor in Physiology
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Department: Physiology
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Graduate Group Affiliations
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Contact information
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630 Clinical Research Building
3b 415 Curie Boulevard
Philadelphia, PA 19104-6085
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3b 415 Curie Boulevard
Philadelphia, PA 19104-6085
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Office: 215 573-3257
32 Fax: 215 573-5851
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32 Fax: 215 573-5851
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Publications
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Education:
21 9 B.S. 1e (Chemistry/History) c
34 College of William and Mary, 1982.
21 a Ph.D. 19 (Biochemistry) c
36 Pennsylvania State University, 1987.
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21 9 B.S. 1e (Chemistry/History) c
34 College of William and Mary, 1982.
21 a Ph.D. 19 (Biochemistry) c
36 Pennsylvania State University, 1987.
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Links
9a Search PubMed for articles
a6 Cell and Molecular Biology graduate group faculty webpage.
83 Physiology Department Faculty Page
5c Holzbaur Lab
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Permanent link9a Search PubMed for articles
a6 Cell and Molecular Biology graduate group faculty webpage.
83 Physiology Department Faculty Page
5c Holzbaur Lab
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24 Microtubule-based motility.
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7c Key words: Dynein, cytoplasmic dynein, dynactin, microtubule motors, microtubules, microtubule dynamics, EB1.
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26 Description of Research
3af Our laboratory is focused on the microtubule-based motor cytoplasmic dynein and its activator dynactin. Dynein and dynactin are required for vesicular trafficking, microtubule organization, mitotic spindle assembly, and development of polarity. We are interested in the mechanisms of force production and motor function, mechanisms of cargo coupling and regulation, effects of dynein and dynactin on dynamics of the cytoskeleton, and the analysis of neurodegenerative diseases resulting from impairments in dynein/dynactin function. Disruptions in dynein/dynactin function cause motor neuron degeneration and muscle atrophy, leading to motor neuron diseases similar to ALS. Approaches in the lab include in vitro motility assays for motors, microtubules and organelles, biochemical and cellular assays for binding partners, live cell microscopy, and development and characterization of transgenic mouse models for motor neuron disease.
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20 Rotation Projects
4d 1. Investigating mechanisms of cargo coupling of cytoplasmic dynein.
62 2. Investigating the coordinated regulation of bidirectional microtubule-based transport.
76 3. Investigating the role of the microtubule plus end-binding protein EB1 in mediating cytoskeletal dynamics.
64 4. Investigating the cellular effects of dynactin mutations linked to motor neuron disease.
4f 5. Investigating the role of axonal transport in motor neuron disease.
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1d Lab personnel:
35 Karen Wallace-Jahn, VMD - Postdoctoral Fellow
33 Sandra Maday, PhD - NIH Postdoctoral Fellow
37 Alison Twelvetrees, PhD - Wellcome Trust Fellow
31 Pallavi Gopal, MD, PhD - Pathology Fellow
32 Amy Ghiretti, PhD - Postdoctoral Scientist
2f Mariko Tokito, MS - Research Specialist
55 Betsy Buechler McIntosh - AHA Predoctoral Fellow, Co-mentored with Mike Ostap
34 Eva Klinman - MD/PhD Candidate, Neuroscience
35 Jeff Nirschl - MD/PhD Candidate, Neuroscience
42 Mara Olenick - Graduate Student, Biochemistry & Biophysics
30 Swathi Ayloo - Graduate Student, Biology
34 Yvette Wong - Graduate Student, Neuroscience
32 Andy Moore - Graduate Student, Neuroscience
26 29
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Description of Research Expertise
2b Research Interests24 Microtubule-based motility.
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7c Key words: Dynein, cytoplasmic dynein, dynactin, microtubule motors, microtubules, microtubule dynamics, EB1.
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26 Description of Research
3af Our laboratory is focused on the microtubule-based motor cytoplasmic dynein and its activator dynactin. Dynein and dynactin are required for vesicular trafficking, microtubule organization, mitotic spindle assembly, and development of polarity. We are interested in the mechanisms of force production and motor function, mechanisms of cargo coupling and regulation, effects of dynein and dynactin on dynamics of the cytoskeleton, and the analysis of neurodegenerative diseases resulting from impairments in dynein/dynactin function. Disruptions in dynein/dynactin function cause motor neuron degeneration and muscle atrophy, leading to motor neuron diseases similar to ALS. Approaches in the lab include in vitro motility assays for motors, microtubules and organelles, biochemical and cellular assays for binding partners, live cell microscopy, and development and characterization of transgenic mouse models for motor neuron disease.
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20 Rotation Projects
4d 1. Investigating mechanisms of cargo coupling of cytoplasmic dynein.
62 2. Investigating the coordinated regulation of bidirectional microtubule-based transport.
76 3. Investigating the role of the microtubule plus end-binding protein EB1 in mediating cytoskeletal dynamics.
64 4. Investigating the cellular effects of dynactin mutations linked to motor neuron disease.
4f 5. Investigating the role of axonal transport in motor neuron disease.
8
1d Lab personnel:
35 Karen Wallace-Jahn, VMD - Postdoctoral Fellow
33 Sandra Maday, PhD - NIH Postdoctoral Fellow
37 Alison Twelvetrees, PhD - Wellcome Trust Fellow
31 Pallavi Gopal, MD, PhD - Pathology Fellow
32 Amy Ghiretti, PhD - Postdoctoral Scientist
2f Mariko Tokito, MS - Research Specialist
55 Betsy Buechler McIntosh - AHA Predoctoral Fellow, Co-mentored with Mike Ostap
34 Eva Klinman - MD/PhD Candidate, Neuroscience
35 Jeff Nirschl - MD/PhD Candidate, Neuroscience
42 Mara Olenick - Graduate Student, Biochemistry & Biophysics
30 Swathi Ayloo - Graduate Student, Biology
34 Yvette Wong - Graduate Student, Neuroscience
32 Andy Moore - Graduate Student, Neuroscience
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e9 Caviston J. P., Holzbaur E. L. F.: Huntingtin as an essential integrator of intracellular vesicular trafficking. Trends in cell biology 19(4): 147-55, Apr 2009.
12d Dixit R, Barnett B, Lazarus JE, Tokito M, Goldman YE, Holzbaur EL.: Microtubule plus-end tracking by CLIP-170 requires EB1. Proceedings of the National Academy of Sciences of the United States of America 106(2): 492-7, Jan 2009.
126 Dixit Ram, Levy Jennifer R, Tokito Mariko, Ligon Lee A, Holzbaur Erika L F: Regulation of dynactin through the differential expression of p150Glued isoforms. The Journal of biological chemistry 283(48): 33611-9, Nov 2008.
f5 Levy Jennifer R, Holzbaur Erika L F: Dynein drives nuclear rotation during forward progression of motile fibroblasts. Journal of cell science 121(Pt 19): 3187-95, Oct 2008.
151 Caviston Juliane P, Ross Jennifer L, Antony Sheila M, Tokito Mariko, Holzbaur Erika L F: Huntingtin facilitates dynein/dynactin-mediated vesicle transport. Proceedings of the National Academy of Sciences of the United States of America 104(24): 10045-50, Jun 2007.
e6 Perlson E, Maday S, Fu MM, Moughamian AJ, Holzbaur EL.: Retrograde axonal transport: pathways to cell death? Trends in Neurosciences 33(7): 335-44, Jul 2010.
10d Hendricks AG, Perlson E, Ross JL, Schroeder HW 3rd, Tokito M, Holzbaur EL: Motor coordination via a tug-of-war mechanism drives bidirectional vesicle transport. Curr Biol 20(8): 697-702, Apr 2010.
e7 Holzbaur E. L. F., Goldman Y. E.: Coordination of molecular motors: from in vitro assays to intracellular dynamics. Curr Opin Cell Biol 22(1): 4-13, Feb 2010.
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Selected Publications
117 Perlson E, Jeong GB, Ross JL, Dixit R, Wallace KE, Kalb RG, Holzbaur EL.: A switch in retrograde signaling from survival to stress in rapid-onset neurodegeneration. J Neuroscience 29(31): 9903-17, Aug 2009.e9 Caviston J. P., Holzbaur E. L. F.: Huntingtin as an essential integrator of intracellular vesicular trafficking. Trends in cell biology 19(4): 147-55, Apr 2009.
12d Dixit R, Barnett B, Lazarus JE, Tokito M, Goldman YE, Holzbaur EL.: Microtubule plus-end tracking by CLIP-170 requires EB1. Proceedings of the National Academy of Sciences of the United States of America 106(2): 492-7, Jan 2009.
126 Dixit Ram, Levy Jennifer R, Tokito Mariko, Ligon Lee A, Holzbaur Erika L F: Regulation of dynactin through the differential expression of p150Glued isoforms. The Journal of biological chemistry 283(48): 33611-9, Nov 2008.
f5 Levy Jennifer R, Holzbaur Erika L F: Dynein drives nuclear rotation during forward progression of motile fibroblasts. Journal of cell science 121(Pt 19): 3187-95, Oct 2008.
151 Caviston Juliane P, Ross Jennifer L, Antony Sheila M, Tokito Mariko, Holzbaur Erika L F: Huntingtin facilitates dynein/dynactin-mediated vesicle transport. Proceedings of the National Academy of Sciences of the United States of America 104(24): 10045-50, Jun 2007.
e6 Perlson E, Maday S, Fu MM, Moughamian AJ, Holzbaur EL.: Retrograde axonal transport: pathways to cell death? Trends in Neurosciences 33(7): 335-44, Jul 2010.
10d Hendricks AG, Perlson E, Ross JL, Schroeder HW 3rd, Tokito M, Holzbaur EL: Motor coordination via a tug-of-war mechanism drives bidirectional vesicle transport. Curr Biol 20(8): 697-702, Apr 2010.
e7 Holzbaur E. L. F., Goldman Y. E.: Coordination of molecular motors: from in vitro assays to intracellular dynamics. Curr Opin Cell Biol 22(1): 4-13, Feb 2010.
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