Matthew C Good, Ph.D.

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Assistant Professor of Cell and Developmental Biology
Department: Cell and Developmental Biology
Graduate Group Affiliations

Contact information
421 Curie Boulevard
Philadelphia, PA 19104
Office: 215-573-1099
Lab: 215-573-6805
BA (Biochemistry)
University of California, Berkeley, 2003.
PhD (Biochemistry)
University of California, San Francisco, 2010.
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Description of Research Expertise

* Adaptability of intracellular structures to variation in cell size and shape
* Temporal and spatial regulation of zygotic genome activation
* Self-assembly of intrinsically disordered proteins
* Phase separation and membraneless organelles
* Subcellular organization of intracellular signaling pathways
* Synthetic cells and protocells, synthetic organelles
* Optochemical protein dimerization

cell size, intracellular size scaling, size regulation, embryogenesis, zygotic genome activation, transcriptional imaging, Xenopus, zebrafish, optochemical dimerization, split enzymes, cell-free systems, encapsulation, microfluidics, emulsions, synthetic cells, IDPs, membraneless organelles


Our lab is interested: 1) cell size control and the downstream consequences of size-dysregulation, and 2) coordinated protein assembly into mesoscale structures, including membraneless organelles, the mitotic spindle and signaling complexes. To investigate the impacts of cell size on cell function, we characterize early embryo development, a period in which cells rapidly divide without growing. Additionally, we are investigating diseased cell states, such as cancer, in which proper cell size homeostasis is lost. To characterize principles that drive the self-assembly of proteins, we focus on scaling and positioning of the mitotic spindle within cleavage stage embryos and in vitro. Additionally we want to understand the properties of proteins that drive their self-assembly and phase separation into membraneless organelles.

We utilize a set of interdisciplinary approaches to address the fundamental questions in cell biology. This strategy requires that we adapt and build new tools, including imaging of transcription during embryo development; microfluidic approaches to encapsulate proteins and cell-free cytoplasmic mixtures inside cell-like compartments in vitro; optochemical dimerizers to control protein localization and enzymatic activity within synthetic cells. Our model systems include: zebrafish and Xenopus cleavage-stage embryos, mammalian cell culture, and synthetic cells. We leverage numerous biochemical approaches extoll the power of bottom-up reconstitution to recapitulate cellular processes ex vivo.

Selected Publications

Schuster B.S., Dignon G.L., Tang W.S., Kelley F.M., Ranganath A.K., Jahnke C.N., Simpkins A.G., Regy R.M., Hammer D.A., Good M.C., Mittal J. : Identifying Sequence Perturbations to an Intrinsically Disordered Protein that Determine Its Phase Separation Behavior. PNAS May 2020.

Chen, H., Good, M.C: Imaging Nascent Transcription in Wholemount Vertebrate Embryos to Characterize Zygotic Genome Activation. Methods In Enzymology April 2020.

Li, S., Xia, B., Javed, B., Hasley, W.D., Melendez-Davila, A., Liu, M., Kerzner, M., Agarwal, S., Xiao, Q., Torre, P., Bermudez, J.G., Rahimi, K., Kostina, N.Y., Möller, M., Rodriguez-Emmenegger,C., Klein, M.L., Percec, V. and Good, M.C.* : Direct Visualization of Vesicle Disassembly and Reassembly Using Photocleavable Dendrimers Elucidates Cargo Release Mechanisms. ACS Nano May 2020.

Reed E.H., Schuster B.S., Good M.C., Hammer D.A: SPLIT: Stable Protein Coacervation Using a Light Induced Transition. ACS Synthetic Biology 9: 500-507, Mar 2020.

Chen H., Einstein, L., Little S., Good M.C.*: Spatiotemporal Patterning of Zygotic Genome Activation in A Model Vertebrate Embryo. Developmental Cell 49(6): 852-866, June 2019.

Paola Torre, Qi Xiao, Irene Buzzacchera, Samuel E. Sherman, Khosrow Rahimi, Nina Yu Kostina, Cesar Rodriguez-Emmenegger, Martin Möller, Christopher J. Wilson, Michael L. Klein, Matthew C. Good*, Virgil Percec*: Encapsulation of Hydrophobic Components in Dendrimersomes and Decoration of their Surface with Proteins and Nucleic Acids. PNAS 116 (31): 15378-15385, July 2019.

Good M.*, Trepat X.*: Cell Parts to Complex Processes from the Bottom Up. Nature 563: 188-189, Nov 2018.

Schuster B.S., Reed E.H., Parthasarathy R., Jahnke C.N., Caldwell R.M., Bermudez J.G., Ramage H., Good M.C.*, Hammer D.A.*: Controllable Protein Phase Separation and Modular Recruitment to Form Responsive, Membraneless Organelles. Nature Communications 9(1): 2985, July 2018.

Caldwell R.M., Bermudez J.G., Thai D., Aonbangkhen C., Schuster B.S., Courtney T., Deiters A., Hammer D.A., Chenoweth D.M., Good M.C.*: Optochemical Control of Protein Localization and Activity within Cell-like Compartments. Biochemistry 57(18): 2590-2596, May 2018.

Good MC, Vahey MD, Skandarajah A, Fletcher DA, Heald R.: Cytoplasmic volume modulates spindle size during embryogenesis. Science 342(6160): 856-60, Nov 2013.

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Last updated: 02/10/2021
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