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David W. Speicher, Ph.D.
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Department: Biochemistry and Biophysics
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Contact information
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The Wistar Institute
40 3601 Spruce Street, Room 272A
Philadelphia, PA 19104
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40 3601 Spruce Street, Room 272A
Philadelphia, PA 19104
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Office: 215-898-3972
32 Fax: 215-495-6915
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32 Fax: 215-495-6915
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Email:
speicher@wistar.org
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speicher@wistar.org
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Education:
21 9 B.S. 19 (Biochemistry) c
36 Pennsylvania State University, 1972.
21 a Ph.D. 19 (Biochemistry) c
36 Pennsylvania State University, 1977.
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21 9 B.S. 19 (Biochemistry) c
36 Pennsylvania State University, 1972.
21 a Ph.D. 19 (Biochemistry) c
36 Pennsylvania State University, 1977.
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Links
9d Proteomics Core Facility, The Wistar Institute
a1 David W. Speicher, Ph.D. Laboratory, The Wistar Institute
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Permanent link9d Proteomics Core Facility, The Wistar Institute
a1 David W. Speicher, Ph.D. Laboratory, The Wistar Institute
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1b Research Interests:
20 * Chemical crosslinking
55 * Modeling and verification of protein complexes using chemical crosslinking
4b * Proteome and systems biology analysis of human cancer metastasis
4e * Proteomic analysis of plasma biomarkers and biosignatures of cancer
2a * Biomarkers of ectopic pregnancy
61 * Proteome analysis of oxidative stress and role of peroxiredoxin 6 in acute lung injury
5e * Macromolecular interaction mechanisms and thermodynamics in complex protein systems
54 * Structure, function, assembly and regulation of membrane skeletal proteins
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Description of Research Expertise
a8 Key Words: cancer biomarkers; tumor progression; proteomics; protein assembly and thermodynamics; membrane skeleton; mass spectrometry; systems biology8
1b Research Interests:
20 * Chemical crosslinking
55 * Modeling and verification of protein complexes using chemical crosslinking
4b * Proteome and systems biology analysis of human cancer metastasis
4e * Proteomic analysis of plasma biomarkers and biosignatures of cancer
2a * Biomarkers of ectopic pregnancy
61 * Proteome analysis of oxidative stress and role of peroxiredoxin 6 in acute lung injury
5e * Macromolecular interaction mechanisms and thermodynamics in complex protein systems
54 * Structure, function, assembly and regulation of membrane skeletal proteins
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145 Gaetani Massimiliano, Mootien Sara, Harper Sandra, Gallagher Patrick G, Speicher David W: Structural and functional effects of hereditary hemolytic anemia-associated point mutations in the alpha spectrin tetramer site. Blood 111(12): 5712-20, Jun 2008.
148 Han Mee-Jung, Herlyn Meenhard, Fisher Aron B, Speicher David W: Microscale solution IEF combined with 2-D DIGE substantially enhances analysis depth of complex proteomes such as mammalian cell and tissue extracts. Electrophoresis 29(3): 695-705, Feb 2008.
13e Li Donghai, Tang Hsin-Yao, Speicher David W: A structural model of the erythrocyte spectrin heterodimer initiation site determined using homology modeling and chemical cross-linking. The Journal of biological chemistry 283(3): 1553-62, Jan 2008.
101 Li Donghai, Harper Sandra, Speicher David W: Initiation and propagation of spectrin heterodimer assembly involves distinct energetic processes. Biochemistry 46(37): 10585-94, Sep 2007.
fd Johnson Colin P, Tang Hsin-Yao, Carag Christine, Speicher David W, Discher Dennis E: Forced unfolding of proteins within cells. Science (New York, N.Y.) 317(5838): 663-6, Aug 2007.
137 Liu Qin, Tan Glenn, Levenkova Natasha, Li Tiansen, Pugh Edward N, Rux John J, Speicher David W, Pierce Eric A: The proteome of the mouse photoreceptor sensory cilium complex. Molecular & cellular proteomics : MCP 6(8): 1299-317, Aug 2007.
17f Hoffman Seth A, Joo Won-A, Echan Lynn A, Speicher David W: Higher dimensional (Hi-D) separation strategies dramatically improve the potential for cancer biomarker detection in serum and plasma. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 849(1-2): 43-52, Apr 2007.
170 Tang Hsin-Yao, Ali-Khan Nadeem, Echan Lynn A, Levenkova Natasha, Rux John J, Speicher David W: A novel four-dimensional strategy combining protein and peptide separation methods enables detection of low-abundance proteins in human plasma and serum proteomes. Proteomics 5(13): 3329-42, Aug 2005.
e6 Tang Hsin-Yao, Speicher David W: In vivo phosphorylation of human erythrocyte spectrin occurs in a sequential manner. Biochemistry 43(14): 4251-62, Apr 2004.
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Selected Publications
136 Wang Huan, Chang-Wong Tony, Tang Hsin-Yao, Speicher David W: Comparison of Extensive Protein Fractionation and Repetitive LC-MS/MS Analyses on Depth of Analysis for Complex Proteomes. Journal of proteome research 9(2): 1032-40, Dec 2009.145 Gaetani Massimiliano, Mootien Sara, Harper Sandra, Gallagher Patrick G, Speicher David W: Structural and functional effects of hereditary hemolytic anemia-associated point mutations in the alpha spectrin tetramer site. Blood 111(12): 5712-20, Jun 2008.
148 Han Mee-Jung, Herlyn Meenhard, Fisher Aron B, Speicher David W: Microscale solution IEF combined with 2-D DIGE substantially enhances analysis depth of complex proteomes such as mammalian cell and tissue extracts. Electrophoresis 29(3): 695-705, Feb 2008.
13e Li Donghai, Tang Hsin-Yao, Speicher David W: A structural model of the erythrocyte spectrin heterodimer initiation site determined using homology modeling and chemical cross-linking. The Journal of biological chemistry 283(3): 1553-62, Jan 2008.
101 Li Donghai, Harper Sandra, Speicher David W: Initiation and propagation of spectrin heterodimer assembly involves distinct energetic processes. Biochemistry 46(37): 10585-94, Sep 2007.
fd Johnson Colin P, Tang Hsin-Yao, Carag Christine, Speicher David W, Discher Dennis E: Forced unfolding of proteins within cells. Science (New York, N.Y.) 317(5838): 663-6, Aug 2007.
137 Liu Qin, Tan Glenn, Levenkova Natasha, Li Tiansen, Pugh Edward N, Rux John J, Speicher David W, Pierce Eric A: The proteome of the mouse photoreceptor sensory cilium complex. Molecular & cellular proteomics : MCP 6(8): 1299-317, Aug 2007.
17f Hoffman Seth A, Joo Won-A, Echan Lynn A, Speicher David W: Higher dimensional (Hi-D) separation strategies dramatically improve the potential for cancer biomarker detection in serum and plasma. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 849(1-2): 43-52, Apr 2007.
170 Tang Hsin-Yao, Ali-Khan Nadeem, Echan Lynn A, Levenkova Natasha, Rux John J, Speicher David W: A novel four-dimensional strategy combining protein and peptide separation methods enables detection of low-abundance proteins in human plasma and serum proteomes. Proteomics 5(13): 3329-42, Aug 2005.
e6 Tang Hsin-Yao, Speicher David W: In vivo phosphorylation of human erythrocyte spectrin occurs in a sequential manner. Biochemistry 43(14): 4251-62, Apr 2004.
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