faculty photo

Hajime Takano, Ph.D.

Research Associate Professor of Neurology
Department: Neurology

Contact information
The Children’s Hospital of Philadelphia
Abramson Research Center
3615 Civic Center Boulevard, Room 409
Philadelphia, PA 19104
Office: 2155901198
B.S. (BiomolecularEngineering)
Tokyo Institute of Technology, Japan , 1992.
M.S. (Bioengineering/Biotechnology)
Tokyo Institute of Technology, Japan , 1994.
Ph.D. (Bioengineering/Biotechnology)
Tokyo Institute of Technology, Japan , 1997.
Post-Graduate Training
Postdoctoral Research Associate, Microanalytical Instrumentation Center, Analytical Chemistry, Iowa State University, Professor Marc D. Porter , 1997-2000.
Postdoctoral Research Associate, Roy J. Carver Laboratory for Ultrahigh Resolution Biological Microscopy, Iowa State University,Professor Marc D. Porter,Professor Philip G Haydon , 2000-2001.
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Description of Research Expertise

Dynamic Cellular Imaging of Nervous System Function: My research focuses on neuroscience imaging applications, particularly working on functional cellular imaging to understand the basic mechanisms of neurological disorders in animal models. I applied a high-speed confocal microscopy (up to ~300Hz frame rate) to examine onset timing of neuronal firing in seizure-like activity in the hippocampus slice. Using a statistical categorization algorithm, i.e., a model based clustering, I was able to shed light on various types of seizure-like activities that expressed both random and organized network bursting. (Takano et.al., Journal of Neuroscience, 2012). I also developed a methodology to conduct simultaneous calcium imaging and voltage-sensitive dye imaging using two-photon microscopy and high-speed CCD camera (~2kHz) (Yu et.al. 2013). I co-first authored the Nature Communication paper to report that the graphene electrode could be used to record neuronal signals while simultaneously conducting calcium imaging (Kuzum*, Takano* et. al., Nature communications, 2014). Recently, I integrated two-photon microscopy into virtual reality environment to study hippocampus place cell in awake behaving mice. Currently, I am working on characterizing hippocampus place cells in disease models such epilepsy and schizophrenia. I am also interested in integrating technologies such as eye-tracking and field potential recording during virtual reality navigation. I am also working on chloride imaging using time-correlated single photon counting based fluorescence lifetime imaging microscopy (FLIM).
In addition to these innovations above, I am contributing to various projects as an expert in biological imaging and analysis. I have instructed more than 80 students, postdocs, and researchers in the UPENN/CHOP neuroscience community in advanced biological imaging techniques such as two-photon microscopy, laser confocal microscopy, FRAP, FRET, and photolysis. In addition to imaging experiments, I am continuously developing various analysis algorithms such as image quantifications, calcium transient detection, peak counts and 3D visualization, automatic image thresholding using a statistical approach, and cluster analysis using various programming platforms such as Matlab and R.
My early research focus was the application of micro- and nanotechnology to chemical and biological analysis using various microscopy techniques including atomic force microscopy (Takano et. al., Chemical Review, 1999), electrostatic force microscopy (Takano, et al., JACS, 2001) and fiber optics-based scanning near-field optical microscopy. I was also a pioneer in applying soft-lithography-based cellular micro-patterns to basic neuroscience research using calcium imaging approach. (Takano, et al., Analytical Chemistry, 2002).

Selected Publications

Yu, E., Dengler, C., Frausto, S., Putt, M., Yue, C., Takano, H., and Coulter, D.A.: Protracted postnatal development of sparse, specific dentate granule cell activation in the mouse hippocampus. Journal of Neuroscience 33(7): 2947-2960, February 2013.

Takano, H., McCartney, M., Ortinski, P., Putt, M., and Coulter, D.A.: Deterministic and stochastic neuronal contributions to distinct synchronous CA3 network bursts. Journal of Neuroscience 32(14): 4743-4754, April 2012.

Takano, H.,Coulter, D.A.: Imaging of hippocampal circuits in epilepsy. Jasper’s Basic Mechanisms of Epilepsies, 3rd Edition. Noebeis, J., Rogawski, M., Delgado-Escueta, A., Avoli, M. (eds.). December 2010.

Ortinski, P. I., Dong, J., Mungenast, A., Yue, C., Takano, H., Watson, D. J., Haydon, P. G., Coulter, D. A.: Selective induction of astrocytic gliosis generates deficits in neuronal inhibition. Nat Neurosci 13(5): 584-91, May 2010.

Halassa, M. M., Fellin, T., Takano, H., Dong, J. H., Haydon, P. G.: Synaptic islands defined by the territory of a single astrocyte. J Neurosci 27(24): 6473-7, June 2007.

Barrett, L. E., Sul, J. Y., Takano, H., Van Bockstaele, E. J., Haydon, P. G., Eberwine, J. H.: Region-directed phototransfection reveals the functional significance of a dendritically synthesized transcription factor. Nat Methods 3(6): 455-60, June 2006.

Mrass, P., Takano, H., Ng, L. G., Daxini, S., Lasaro, M. O., Iparraguirre, A., Cavanagh, L. L., von Andrian, U. H., Ertl, H. C., Haydon, P. G., Weninger, W.: Random migration precedes stable target cell interactions of tumor-infiltrating T cells. J Exp Med 203(12): 2749-61, November 2006.

Takano, H, Sul, JY, Mazzanti Y, Doyle, RT, Haydon, PG and Porter,MD: Micropatterned substrates: approach to probing intercellular communication pathways. Analytical Chemistry 74(18): 4640-4646, September 2002.

Takano, H., Porter, M. D.: Monitoring chemical transformations at buried organic interfaces by electric force microscopy. J Am Chem Soc 123(34): 8412-3, August 2001.

Takano,H., Kenseth,J.R., Wong, S.S., O'Brien,J.C., Porter, M.D.: Chemical and Biological Analysis using Scanning Force Microscopy. Chemical Review 99: 2845-2890, September 1999.

Deng, H., Van Berkel, G. J., Takano, H., Gazda, D., Porter, M. D.: Electrochemically modulated liquid chromatography coupled on-line with electrospray mass spectrometry. Analytical Chemistry 72(11): 2641-7, June 2000.

Takano, H., Fujihira, M.: AFM microlithography of a thin chromium film covered with a thin resist Langmuir-Blodgett (LB) film. Thin Solid Films 273(1-2): 312-316, 1996.

Overney, R. M., Meyer, E., Frommer, J., Brodbeck, D., Luthi, R., Howald, L., Guntherodt, H. J., Fujihira, M., Takano, H., Gotoh, Y.: Friction measurements on phase-separated thin-films with a modified atomic force microscope. Nature 359(6391): 133-135, September 1992.

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Last updated: 01/13/2023
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