Instruments Available for Use/Access*
| Cell Prep | |||
|---|---|---|---|
| Instrument Name | Specs | Location | Access |
| Cellaca (cell counter & viability) | Brightfield and 2 LEDs: Green (Live cells or GFP) and Red (Dead cells); nuclei only option. Order 12x2 plates and reagent here | 297 JMB | 24/7 trained users |
| gentleMACS (tissue dissociator w/ heaters) | Tubes and enzyme kits | 297 JMB | Email Nicolle for consultation/access: nicollem@miltenyi.com |
| Rhapsody | Single-cell analysis system | 231 JMB | 9-5pm M-F trained users |
| Sorters | |||
|---|---|---|---|
| Cytometer Name | Configuration | Location | Access |
| Symphony S6 | 5 Lasers: UV (8), Vio (8), Blue (6), YG (5), Red (3) = 30 colors | 206 JMB | 12-5pm M-F assisted sorts or 24/7 trained users |
| Discover S8 w/ imaging | 5 Lasers: UV (22), Vio (20), Blue (16), YG (12), Red (8) = 78 colors | 298 JMB | Staff run only. Call to book time |
| Aria B (nonhuman/noninfectious only) | 4 Lasers: Vio (4), Blue (2), Grn (5), Red (3) = 14 colors | 206 JMB | 24/7 trained users |
| Aria C | 4 Lasers: Vio (5), Blue (2), Grn (5), Red (3) = 15 colors | 362 BRB | 24/7 trained users |
| Aria D (BSL2+ sorting) | 4 Lasers: Vio (5), Blue (2), Grn (5), Red (3) = 15 colors | 578 BRB | 12-5pm M-F assisted sorts or 24/7 trained/approved users |
| AriaFusion CJ | 4 Lasers: Vio (6), Blue (2), YG (5), Red (3) = 16 colors | 8-173A TRC | Restricted: June members only |
| AriaFusion ES | 5 Lasers: UV (3), Vio (6), Blue (2), YG (4), Red (3) = 18 colors | 390 Towne Bldg | Restricted: SEAS members only |
| AriaFusion B | 4 Lasers: Vio (3), Blue (2), YG (4), Red (2) = 11 colors | 12-252 TRC | 24/7 trained users. Access required |
| Influx A | 4 Lasers: Vio (6), Blue (4), Grn (4), Red (3) = 17 colors | 206 JMB | Staff run only. Call to book time |
| Influx B | 4 Lasers: Vio (7), Blue (2), YG (4), Red (3) = 16 colors | 298 JMB | Staff run only. Call to book time |
| Melody | 3 Lasers: Blue (2), YG (4), Red (2) = 8 colors | 472 Hill | 24/7 trained users. Access required |
| LSR II | |||
|---|---|---|---|
| Cytometer Name | Configuration | Location | Access |
| LSR A | 4 Lasers: Vio (8), Blue (2), Grn (5), Red (3) = 18 colors | 297 JMB | 24/7 trained users |
| LSR B | 4 Lasers: UV (2), Vio (4), Blue (5), Red (3) = 14 colors | 297 JMB | 24/7 trained users |
| LSR F | 4 Lasers: Vio (6), Blue (2), Grn (5), Red (3) = 16 colors | 332 BRB | 24/7 trained users |
| Fortessa | |||
|---|---|---|---|
| Cytometer Name | Configuration | Location | Access |
| Fortessa Vet | 4 Lasers: Vio (6), Blue (2), Grn (5), Red (3) = 16 colors | 372 Hill | 24/7 trained users. Access required |
| Fortessa B | 5 Lasers: UV (2), Vio (6), Blue (2), YG (5), Red (3) = 18 colors | 249 Stemmler | Trained users 9-5pm M-F. Restricted access nights/weekends |
| Fortessa C | 5 Lasers: UV (2), Vio (6), Blue (2), YG (5), Red (3) = 18 colors | 8-300 South Tower | Restricted. Parker members only |
| Fortessa D w/ HTS | 5 Lasers: UV (2), Vio (6), Blue (2), YG (5), Red (3) = 18 colors | 158a Stemmler | Trained users 11-3pm M-F, card access required. Restricted access nights/weekends: Bar-Or members only |
| Fortessa E w/ HTS | 5 Lasers: UV (5), Vio (5), Blue (2), YG (3), Red (3) = 18 colors | 8-183 TRC | Restricted: Riley members only |
| Fortessa CJ w/ HTS | 4 Lasers: Vio (7), Blue (2), Grn (5), Red (3) = 17 colors | 8-188 TRC | Restricted: June members only |
| Fortessa CJ2 | 4 Lasers: Vio (6), Blue (2), Grn (5), Red (3) = 16 colors | 8-307 South Tower | Restricted: June members only |
| Fortessa Gill w/ HTS | 4 Lasers: Vio (7), Blue (2), YG (4), Red (3) = 16 colors | 8-195 TRC | Restricted: Gill/Powell members only |
| Lyric | |||
|---|---|---|---|
| Cytometer Name | Configuration | Location | Access |
| Lyric | 3 Lasers: Vio (5), Blue (4), Red (3) = 12 colors | 233 JMB | 9-5pm trained users |
*Access to Abramson Cancer Center (ACC) Shared Resources: For each Shared Resource, ACC member satisfaction with access is surveyed annually. There is no discount to ACC members vs. non-member Penn faculty, but access priority differs. Highest priority is given to ACC members with peer-reviewed funded projects, followed by ACC members with other funded projects and non-ACC members with peer-reviewed funded projects (for PSOM Core Facilities), non-ACC members with funded projects, and finally users from outside the institution.