Michael Ostap, Ph.D.
Michael Ostap, Ph.D.
Professor of Physiology
Department: Physiology
Graduate Group Affiliations
Contact information
Mail: B400 Richards Bldg.
Office/Lab: B40 Anat-Chem
3700 Hamilton Walk/6085
Philadelphia, PA 19104
Office/Lab: B40 Anat-Chem
3700 Hamilton Walk/6085
Philadelphia, PA 19104
Office: (215) 573-9758
Email:
ostap@mail.med.upenn.edu
ostap@mail.med.upenn.edu
Publications
Education:
B.S. (Chemistry)
Illinois State University, 1988.
Ph.D. (Biochemistry, Molecular Biology, and Biophysics)
University of Minnesota, 1993.
Permanent linkB.S. (Chemistry)
Illinois State University, 1988.
Ph.D. (Biochemistry, Molecular Biology, and Biophysics)
University of Minnesota, 1993.
Description of Research Expertise
Research InterestsMolecular mechanisms of cell motility
Key words: Cell motility, myosin, actin, biochemistry, single molecule, spectroscopy, microscopy, fluorescence, nanotechnology.
Description of Research
The goal of our research is to understand the molecular mechanisms of cell motility, which include: cell migration, regulation of cell shape and extracellular attachment, and intracellular transport. Cell motility is crucial for several normal and pathological processes, including: cell and tissue development, endocytosis, wound healing, immune response, and metastases of tumors.
Lab Personnel
Jennine Dawicki McKenna, CAMB Student
Elizabeth Feeser, Post-doctoral
Joseph Laakso, BMB Student
Tianming Lin, Research Specialist
Serapion Pyrpassopoulos, Post-doctoral
Selected Publications
J.M. Dawicki McKenna and E.M. Ostap: Kinetics of the Interaction of Myo1c with Phosphoinositides. J. Biol. Chem. 284(42): 28650-28659, 2009.E.M. De La Cruz and E.M. Ostap: Kinetic and Equilibrium Analysis of the Myosin ATPase. Methods in Enzymology 455: 157-192, 2009.
Y. Sun, J. Dawicki McKenna, J. Murry, E.M. Ostap, Y.E. Goldman: Parallax: High Accuracy Three-Dimensional Single Molecule Tracking Using Split Images. Nano Letters 9(7): 2676-2682, 2009.
J.M. Laakso, J.H. Lewis, H. Shuman, and E.M. Ostap: Myosin-I Can Act as a Molecular Force Sensor. Science 321: 133-136, 2008.
E.M. Ostap: Tropomyosins as Discriminators of Myosin Function. Tropomyosins. Peter Gunning (eds.). Landes Bioscience, 2008.
E.M. De La Cruz and E.M. Ostap: Actin. Cells. Benjamin Lewin (eds.). Jones and Bartlett, 2007 Notes: Textbook for Graduate and Advanced Undergraduate Students.
S. Manceva, T. Lin, H. Pham, J.H. Lewis, Y.E. Goldman, and E.M. Ostap: Calcium Regulation of Calmodulin Binding to and Dissociation from the Myo1c Regulatory Domain. Biochemistry 48: 11718-11726, 2007.
D.E. Hokanson, J.M. Laakso, T. Lin, D. Sept, and E.M. Ostap: Myo1c Binds Phosphoinositides through a Putative PH Domain. Mol. Biol. Cell 17: 4856-4865, 2006.
D.E. Hokanson and E.M. Ostap: Myo1c Binds Tightly and Specifically to PIP2 and InsP3. Proc. Natl. Acad. Sci. 103: 3118-3123, 2006.
J.H. Lewis, T. Lin, D.E. Hokanson, and E.M. Ostap: Temperature Dependence of Nucleotide Association and Kinetic Characterization of Myo1b. Biochemistry 45: 11589-11597, 2006 Notes: Identified as a "Hot Article" by the American Chemical Society.