Description of Research Expertise

Complex I (NADH-quinone oxidoreductase) is the largest transmembrane multi-subunit enzyme in the mitochondrial and bacterial respiratory chain. It transports electrons from NADH to quinone, and simultaneously transfers protons across the membrane to build up electrochemical proton gradient. This subsequently drives ATP synthesis. Complex I is also considered to be a major site for generation of reactive oxygen species (ROS), which may be associated with neurodegenerative diseases, Parkinson’s disease, as well as aging and apoptosis.

The major interest of my research group is the unique coupling between the electron transfer and proton pumping mechanism of complex I which covers both bacterial and mitochondrial systems, utilizing a wide range of research strategies, from molecular biology, protein biochemistry, enzymology, in combination with various CW- and pulse electron paramagnetic resonance techniques. We also investigate ROS generation mechanism and its relevance to the above mentioned human diseases.