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Deborah French, PhD

Deborah French, PhD

faculty photo
Research Associate Professor of Pathology and Laboratory Medicine
Department: Pathology and Laboratory Medicine

Contact information
Children's Hospital of Philadelphia
3501 Civic Center Blvd
Colket Translational Research Building 5014
Philadelphia, PA 19104
Office: 267-426-9673
Lab: 267-426-9676
BS (Microbiology)
Purdue University, W Lafayette, IN, 1974.
PhD (Immunology)
Rush University, Chicago, IL, 1984.
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Perelman School of Medicine > Faculty > Search

Description of Research Expertise

As Director of the human induced pluripotent stem cell (iPSC) core facility within the Center for Cellular and Molecular Therapeutics at The Children’s Hospital of Philadelphia, our mission is to provide expertise and quality-control reagents for culture and differentiation of iPSCs and human embryonic stem cells (hESC) to the research communities. We have established standardized protocols to: 1) Propagate human ESC and iPSC lines, monitor for maintenance of the pluripotent state and distribute these lines to investigators; 2) Differentiate hESCs and iPSCs into the various germ layers and their derivative tissues under controlled, serum-free conditions with defined cytokines; and 3) Generate iPSCs from fibroblasts and blood cells obtained from human subjects using integrating and non-integrating reprogramming systems. We have generated over 60 normal and disease-specific iPSC lines in which the normal reference lines are available for distribution to the research community. In addition to working with investigators to design new protocols tailored for their individualized research, we provide a hands on training course to gain expertise in the growth, maintenance, expansion, and cryopreservation of hESCs and iPSCs. Technologies for creating and manipulating ESCs/iPSCs are evolving rapidly and we are dedicated to incorporating the latest technologies and protocols to the research community.

Selected Publications

Yuhuan Wang, Vincent Hayes, Danuta Jarocha, Xiuli Sim, Dawn C. Harper, Rudy Fuentes, Spencer K. Sullivan, Paul Gadue, Stella T. Chou, Beverly J. Torok-Storb, Michael S. Marks, Deborah L. French, and Mortimer Poncz: Comparative analysis of human ex vivo-generated platelets vs megakaryocyte-generated platelets in mice: a cautionary tale. Blood 2015.

Ji-Yoon Noh, Shilpa Gandre-Babbe, Yuhuan Wang, Vincent Hayes, Yu Yao, Paul Gadue, Spencer K. Sullivan, Stella T. Chou, Kellie R. Machlus, Joseph E. Italiano Jr., Michael Kyba, David Finkelstein, Jacob C. Ulirsch, Vijay G. Sankaran, Deborah L. French, Mortimer Poncz, and Mitchell J. Weiss: Inducible Gata1 suppression expands erythromegakaryocytic progenitors from embryonic stem cells. J Clin Invest 2015.

Marta Byrska-Bishop, Daniel VanDorn, Amy E. Campbell, Marisol Betensky, Philip R. Arca, Yu Yao, Paul Gadue, Fernando F. Costa, Richard L. Nemiroff, Gerd A. Blobel,2 Deborah L. French,4 Ross C. Hardison,1 Mitchell J. Weiss, and Stella T. Chou: Pluripotent stem cells reveal erythroid-specific activities of the GATA1 N-terminus. J Clin Invest 125: 993-1005, 2015.

Xiuli Sim, Fabian L. Cardenas-Diaz, Deborah L. French, and Paul Gadue: A Doxycycline-Inducible System for Genetic Correction of iPSC Disease Models. Methods in Mol Biol 2015.

Tiyaboonchai A, Mac H, Shamsedeen R, Mills JA, Kishore S, French DL, Gadue P: Utilization of the AAVS1 safe harbor locus for hematopoietic specific transgene expression and gene knockdown in human ES cells. Stem Cell Res 12: 630-637, 2014.

Sullivan SK, Mills JA, Koukouritaki SB, Vo KK, Lyde RB, Paluru P, Zhao G, Sullivan LM, Wang Y, Kishore S, Gharaibeh EZ, Lambert MP, Wilcox DA, French DL, Poncz M, Gadue P. : High-level transgene expression in induced pluripotent stem cell-derived megakaryocytes: correction of Glanzmann thrombasthenia. Blood 123: 753-757, 2014.

Mills JA, Paluru P, Weiss MJ, Gadue P, French DL: Hematopoietic differentiation of pluripotent stem cells in culture. Methods in Mol Biol. Bunting KD and Qu C-K (eds.). Humana Press, NY, NY 1185: 181-194, 2014.

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Last updated: 10/02/2015
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