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Deborah French, PhD

Deborah French, PhD

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Research Associate Professor of Pathology and Laboratory Medicine
Department: Pathology and Laboratory Medicine

Contact information
Children's Hospital of Philadelphia
3501 Civic Center Blvd
Colket Translational Research Building 5014
Philadelphia, PA 19104
Office: 267-426-9673
Fax: 215-590-3660
Lab: 267-426-9676
Education:
BS (Microbiology)
Purdue University, West Lafayette, IN, 1974.
PhD (Immunology)
Rush University, Chicago, IL, 1984.
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Description of Research Expertise

As Director of the human induced pluripotent stem cell (iPSC) core facility within the Center for Cellular and Molecular Therapeutics at The Children’s Hospital of Philadelphia, our mission is to provide expertise and quality-control reagents for culture and differentiation of iPSCs and human embryonic stem cells (hESC) to the research communities. We have established standardized protocols to: 1) Propagate human ESC and iPSC lines, monitor for maintenance of the pluripotent state and distribute these lines to investigators; 2) Differentiate hESCs and iPSCs into the various germ layers and their derivative tissues under controlled, serum-free conditions with defined cytokines; and 3) Generate iPSCs from fibroblasts and blood cells obtained from human subjects using integrating and non-integrating reprogramming systems. We have generated over 60 normal and disease-specific iPSC lines in which the normal reference lines are available for distribution to the research community. In addition to working with investigators to design new protocols tailored for their individualized research, we provide a hands on training course to gain expertise in the growth, maintenance, expansion, and cryopreservation of hESCs and iPSCs. Technologies for creating and manipulating ESCs/iPSCs are evolving rapidly and we are dedicated to incorporating the latest technologies and protocols to the research community.

Selected Publications

Chou ST, Byrska-Bishop M, Tober JM, Yao Y, VanDorn D, Opalinska JB, Mills JA, Choi JK, Speck NA, Gadue P,5, Hardison RC, Nemiroff RL, French DL, Weiss MJ: Trisomy 21 associated defects in human primitive hematopoiesis revealed through induced pluripotent stem cells. Proc Natl Acad Sci, USA 109: 17573-17578, 2012.

Cheng X, Ying L, Lu L, DiSimone A, Mills J, Lin HC, Kotton DN, Shen SS, Nostro C, Choi JK, Weiss MJ, French DL, Gadue P: Self-renewing endodermal progenitor lines generated from human pluripotent stem cells. Cell Stem Cell 10: 371-384, 2012.

Gandre-Babbe S, Paluru P, Aribeana C, Chou ST, Bresolin S, Sullivan SK, Tasian SK, Weng J, Favre H, Choi JK, French DL, Loh ML, Weiss MJ: Patient-derived induced pluripotent stem cells recapitulate hematopoietic abnormalities of Juvenile Myelomonocytic Leukemia. Blood 121: 4925-4929, 2013.

Garçon L, Ge J, Manjunath SH, Mills JA, Apicella M, Parikh S, Sullivan LM, Podsakoff GM, Gadue P, French DL, Mason PJ, Bessler M, Weiss MJ: Ribosomal and Hematopoietic Defects in Induced Pluripotent Stem Cells Derived From Diamond Blackfan Anemia Patients. Blood 122: 912-922, 2013.

Paluru P, Hudock KM, Cheng X, Mills JA, Ying L, Galvão AM, Liu L, Tiyaboonchai A, Sim X, Sullivan SK, French DL, Gadue P: The negative impact of Wnt signaling on megakaryocyte and primitive erythroid progenitors derived from human embryonic stem cells. Stem Cell Res 12: 441-451, 2013.

Sullivan SK, Mills JA, Koukouritaki SB, Vo KK, Lyde RB, Paluru P, Zhao G, Sullivan LM, Wang Y, Kishore S, Gharaibeh EZ, Lambert MP, Wilcox DA, French DL, Poncz M, Gadue P. : High-level transgene expression in induced pluripotent stem cell-derived megakaryocytes: correction of Glanzmann thrombasthenia. Blood 123: 753-757, 2014.

Tiyaboonchai A, Mac H, Shamsedeen R, Mills JA, Kishore S, French DL, Gadue P: Utilization of the AAVS1 safe harbor locus for hematopoietic specific transgene expression and gene knockdown in human ES cells. Stem Cell Res 12: 630-637, 2014.

Mills JA, Paluru P, Weiss MJ, Gadue P, French DL: Hematopoietic differentiation of pluripotent stem cells in culture. Hematopoietic Stem Cell Protocols Third Edition. Bunting KD and Qu C-K (eds.). Humana Press, NY, NY Page: 181-194, 2014.

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Last updated: 08/22/2014
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