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Deborah French, PhD

Deborah French, PhD

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Research Associate Professor of Pathology and Laboratory Medicine
Department: Pathology and Laboratory Medicine

Contact information
Children's Hospital of Philadelphia
3501 Civic Center Blvd
Colket Translational Research Building 5014
Philadelphia, PA 19104
Office: 267-426-9673
Fax: 215-590-3660
Lab: 267-426-9676
Education:
BS (Microbiology)
Purdue University, West Lafayette, IN, 1974.
PhD (Immunology)
Rush University, Chicago, IL, 1984.
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Description of Research Expertise

As Director of the human induced pluripotent stem cell (iPSC) core facility within the Center for Cellular and Molecular Therapeutics at The Children’s Hospital of Philadelphia, our mission is to provide expertise and quality-control reagents for culture and differentiation of iPSCs and human embryonic stem cells (hESC) to the research communities. We have established standardized protocols to: 1) Propagate human ESC and iPSC lines, monitor for maintenance of the pluripotent state and distribute these lines to investigators; 2) Differentiate hESCs and iPSCs into the various germ layers and their derivative tissues under controlled, serum-free conditions with defined cytokines; and 3) Generate iPSCs from fibroblasts and blood cells obtained from human subjects using integrating and non-integrating reprogramming systems. We have generated over 60 normal and disease-specific iPSC lines in which the normal reference lines are available for distribution to the research community. In addition to working with investigators to design new protocols tailored for their individualized research, we provide a hands on training course to gain expertise in the growth, maintenance, expansion, and cryopreservation of hESCs and iPSCs. Technologies for creating and manipulating ESCs/iPSCs are evolving rapidly and we are dedicated to incorporating the latest technologies and protocols to the research community.

Selected Publications

Chou ST, Byrska-Bishop M, Tober JM, Yao Y, VanDorn D, Opalinska JB, Mills JA, Choi JK, Speck NA, Gadue P,5, Hardison RC, Nemiroff RL, French DL, Weiss MJ: Trisomy 21 associated defects in human primitive hematopoiesis revealed through induced pluripotent stem cells. Proc Natl Acad Sci, USA 109: 17573-17578, 2012.

Cheng X, Ying L, Lu L, DiSimone A, Mills J, Lin HC, Kotton DN, Shen SS, Nostro C, Choi JK, Weiss MJ, French DL, Gadue P: Self-renewing endodermal progenitor lines generated from human pluripotent stem cells. Cell Stem Cell 10: 371-384, 2012.

Outten JT, Gadue P, French DL, Diamond SL: High-throughput screening assay for embryoid body differentiation of human embryonic stem cells. Current Protocol Stem Cell Biology chapter 1(Unit 1D.6), 2012.

Outten JT, Cheng X, Gadue P, French DL, Diamond SL: A high throughput multiplexed screening assay for optimizing serum-free differentiation protocols of human embryonic stem cells. Stem Cell Research 6: 129, 2011.

Jean JM, smith BW, Lafyatis RA, Demierre MF, Weiss DJ, French DL, Gadue P, Murphy GJ, Mostoslavsky G, Kotton DN: Generation of transgene-free lung disease-specific human iPS cells using a single excisable lentiviral stem cell cassette. Stem Cells 28: 1728-1740, 2010.

Zhou Z, French DL, Ma G, Eisenstein S, Chen Y, Divino CM, Keller G, Chen SH, Pan PY: Development and function of myeloid-derived suppressor cells generated from mouse embryonic and hematopoietic stem cells. Stem Cells 28: 620-632, 2010.

Coller BS, French DL, Rao K. : Hereditary Qualitative Platelet Disorders. in Williams Hematology, Eighth Edition. Beutler E, Lichtman MA, Coller BA, Kipps TJ, Seligsohn U (eds.). McGraw-Hill, New York, NY, 2009.

Artoni A, Li J, Mitchell B, Ruan J, Takagi J, Springer TA, French DL, Coller BS: Integrin β3 regions controlling binding of murine mAb 7E3: Implications for the mechanism of integrin αIIbβ3 activation. PNAS USA 101: 13114-13120, 2004.

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Last updated: 03/31/2014
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