Greg J. Bashaw, Ph.D.

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Professor of Neuroscience
Department: Neuroscience
Graduate Group Affiliations

Contact information
1113 BRB II/III Building
Department of Neuroscience
421 Curie Blvd.
Philadelphia, PA 19104
Office: 215 898-0829
Fax: 215 573-7601
Education:
B.A. (Biology)
Brown University, 1990.
Ph.D. (Biological Sciences)
Stanford University, Mentor: Bruce Baker, 1997.
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Description of Research Expertise

Research Interests
- Molecular mechanisms of axon growth and guidance during nervous system development.
- How axon guidance receptors specify attractive and repulsive signals and transmit these signals to the navigating growth cone.
- Formation of Neural Circuits at the Midline

Key words: Axon guidance, developmental neuroscience, Slit, Robo, Netrin.

Description of Research
How axons in the developing nervous system successfully navigate to their correct targets is a fundamental problem in neurobiology. Understanding the mechanisms that mediate axon guidance will give important insight into how the nervous system is correctly wired during development and may have implications for therapeutic approaches to developmental brain disorders and nerve regeneration. Achieving this understanding will require unraveling the molecular logic that ensures the proper expression and localization of axon guidance cues and receptors, and elucidating the signaling events that regulate the growth cone cytoskeleton in response to guidance receptor activation.

The Slit ligand and Roundabout (Robo) receptors, and the Netrin ligand and DCC/UNC5 receptors are two important evolutionary conserved ligand/receptor systems that contribute to proper connectivity in both the vertebrate and invertebrate nervous systems. These molecules are also known to influence neuronal and mesodermal cell migration, suggesting that determining their function may have broad implications for understanding diseases of nervous system development, many of which have their root in defective cell migration and/or axon guidance. The research in my laboratory addresses the dynamics of axon guidance receptor expression and signaling, and exploits the powerful genetic and molecular approaches available in Drosophila.

Rotation Projects
A range of projects relating to axon guidance receptor regulation and signaling, employing a broad range of techniques in genetics, molecular biology and biochemistry. For specific projects contact Dr. Bashaw.

Lab personnel:

Timothy Evans, Ph.D., Post-doctoral Fellow
Michael O'Donnell, CAMB Graduate Student
Rebecca Chance, Neuroscience Graduate Student
Alexandra Neuhaus-Follini, Neuroscience Graduate Student
Melissa Hernandez, Neuroscience Rotation Student (Fall 2010)
Celine Santiago, CAMB Fall Rotation Student (Fall 2010)
Michael Fleming, Neuroscience Rotation Student (Spring 2011)
Joseph Zinski, CAMB Rotation Student (Spring 2011)
Seth Johnson, CAMB Rotation Student (Spring 2011)
Jonathan Levin, Research Specialist

Selected Publications

Coleman Hope A, Labrador Juan-Pablo, Chance Rebecca K, Bashaw Greg J: The Adam family metalloprotease Kuzbanian regulates the cleavage of the roundabout receptor to control axon repulsion at the midline. Development 137(14): 2417-26, Jul 2010.

Timothy A. Evans and Greg J. Bashaw: Functional Diversity of Robo Receptor Immunoglobulin Domains Promotes Distinct Axon Guidance Decisions. Current Biology 20(6), March 2010.

Yang Long, Garbe David S, Bashaw Greg J: A frazzled/DCC-dependent transcriptional switch regulates midline axon guidance. Science 324(5929): 944-7, May 2009.

Garbe, D.S., O’Donnell, M., and Bashaw, G.J.: Cytoplasmic Domain Requirements for Frazzled-mediated Attractive Axon Turning at the Drosophila Midline. Development 134: 4315-4324. December 2007.

David S. Garbe and Greg J. Bashaw: Independent Functions of Slit-Robo Repulsion and Netrin-Frazzled Attraction Regulate Axon Crossing at the Midline in Drosophila. Journal of Neuroscience 27(13): 3584-3592, March 2007.

Garbe, D.S., Das, A., Dubreuil, R.R. and Bashaw, G.J.: Beta spectrin functions independently of Ankyrin to regulate the establishment and maintenance of axon connections in the Drosophila embryonic CNS. Development 134: 273-284, January 2007.

Yang, L. and Bashaw, G.J.: Son of Sevenless directly links the Robo receptor to Rac activation to control axon repulsion at the midline. Neuron 52(4): 595-607, November 2006.

Labrador, J.P., O'Keefe, D., Yoshikawa, S., McKinnon, R.D., Thomas, J.B. and Bashaw, G.J.: The homeobox transcription factor even-skipped regulates Netrin-receptor expression to control dorsal motor-axon projections in Drosophila. Current Biology 15: 1413-1419, 2005.

Hu, H., Li, M., Labrador, J.P., McEwen, J., Lai, E.C., Goodman, C.S. and Bashaw, G.J.: Cross GAP/Vilse links the Roundabout receptor to Rac to regulate midline repulsion. PNAS 102: 4613-4618, 2005.

Fan, X., Labrador, J.P., Hing, H. and Bashaw, G.J.: Slit stimulation recruits Dock and Pak to the Roundabout receptor and increases Rac activity to regulate axon repulsion at the CNS midline. Neuron 41: 113-127, 2003.

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Last updated: 03/14/2014
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