Michael C. Milone

faculty photo
Assistant Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
Staff Physician, Department of Pathology and Laboratory Medicine
Department: Pathology and Laboratory Medicine

Contact information
3400 Spruce Street 7103 Founders Pavilion
Philadelphia, PA 19104
Office: 215-662-6575
Fax: 215-662-7529
Graduate Group Affiliations
B.S (Chemical Biology)
Stevens Institute of Technology, 1993.
Ph.D (Experimental Pathology/ Immunology)
UMDNJ - Graduate School of Biomedical Sciences, 1999.
M.D. (Medical Doctor)
UMDNJ- New Jersey Medical School, 1999.
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Description of Research Expertise

Research in my laboratory aims toward improving cancer and transplantation therapy through the development of new immunotherapeutic modalities. Active research projects in my laboratory include:

1) Developing improved chimeric antigen receptors (CARs) for adoptive immunotherapy of cancer.

These projects are focused upon understanding the mechanisms of signal transduction of artificial chimeric antigen receptors through traditional biochemical studies along with high resolution dynamic imaging of artificial and natural T cell receptors. New CAR designs are also under development to permit more controlled activation of T cells.

2) Developing novel T cell stimulatory substrates based upon nano- and micro-fabricated surfaces for control of T cell proliferation and differentiation.

This project is part of a collaborative effort supported by the NIH Common Fund. Details of this project can be found at the center's website (www.mechanicalbiology.org) or the NIH common fund site (http://commonfund.nih.gov/nanomedicine/devcenters/mechanicalbiology.asp)

Description of Itmat Expertise

My laboratory is focused upon developing methods to use the immune system to treat cancer. Active research includes 1) exploring the mechanical properties of artificial adhesion substrates used in T cell culture and their influence on T cell biology, 2) development of artificial antigen receptors and signaling systems for control of T cell specificity

Selected Publications

Porter David L, Hwang Wei-Ting, Frey Noelle V, Lacey Simon F, Shaw Pamela A, Loren Alison W, Bagg Adam, Marcucci Katherine T, Shen Angela, Gonzalez Vanessa, Ambrose David, Grupp Stephan A, Chew Anne, Zheng Zhaohui, Milone Michael C, Levine Bruce L, Melenhorst Jan J, June Carl H: Chimeric antigen receptor T cells persist and induce sustained remissions in relapsed refractory chronic lymphocytic leukemia. Science translational medicine 7(303): 303ra139, Sep 2015.

Wang Enxiu, Wang Liang-Chuan, Tsai Ching-Yi, Bhoj Vijay, Gershenson Zack, Moon Edmund, Newick Kheng, Sun Jing, Lo Albert, Baradet Timothy, Feldman Michael D, Barrett David, Puré Ellen, Albelda Steven, Milone Michael C: Generation of Potent T-cell Immunotherapy for Cancer Using DAP12-Based, Multichain, Chimeric Immunoreceptors. Cancer immunology research 3(7): 815-26, Jul 2015.

Xiang Yan, Stine Zachary E, Xia Jinsong, Lu Yunqi, O'Connor Roddy S, Altman Brian J, Hsieh Annie L, Gouw Arvin M, Thomas Ajit G, Gao Ping, Sun Linchong, Song Libing, Yan Benedict, Slusher Barbara S, Zhuo Jingli, Ooi London L, Lee Caroline G L, Mancuso Anthony, McCallion Andrew S, Le Anne, Milone Michael C, Rayport Stephen, Felsher Dean W, Dang Chi V: Targeted inhibition of tumor-specific glutaminase diminishes cell-autonomous tumorigenesis. The Journal of clinical investigation 125(6): 2293-306, Apr 2015.

Frigault Matthew J, Lee Jihyun, Basil Maria Ciocca, Carpenito Carmine, Motohashi Shinichiro, Scholler John, Kawalekar Omkar U, Guedan Sonia, McGettigan Shannon E, Posey Avery D, Ang Sonny, Cooper Laurence J N, Platt Jesse M, Johnson F Brad, Paulos Chrystal M, Zhao Yangbing, Kalos Michael, Milone Michael C, June Carl H: Identification of Chimeric Antigen Receptors That Mediate Constitutive or Inducible Proliferation of T Cells. Cancer immunology research 3(4): 356-67, Jan 2015.

Moon Edmund K, Wang Liang-Chuan, Dolfi Douglas V, Wilson Caleph B, Ranganathan Raghuveer, Sun Jing, Kapoor Veena, Scholler John, Puré Ellen, Milone Michael C, June Carl H, Riley James L, Wherry E John, Albelda Steven M: Multifactorial T-cell Hypofunction That Is Reversible Can Limit the Efficacy of Chimeric Antigen Receptor-Transduced Human T cells in Solid Tumors. Clinical cancer research : an official journal of the American Association for Cancer Research 20(16): 4262-73, Aug 2014.

Bashour Keenan T, Tsai Jones, Shen Keyue, Lee Joung-Hyun, Sun Eileen, Milone Michael C, Dustin Michael L, Kam Lance C: Crosstalk between CD3 and CD28 is spatially modulated by protein lateral mobility. Molecular and Cellular Biology Page: 955-64, Mar 2014.

Cardamone David, Milone Michael C, Glaser Laurel, Frey Noelle V, Kricka Larry J: Eltrombopag and serum of a different hue. Archives of pathology & laboratory medicine 137(9): 1175, Sep 2013.

Grupp Stephan A, Kalos Michael, Barrett David, Aplenc Richard, Porter David L, Rheingold Susan R, Teachey David T, Chew Anne, Hauck Bernd, Wright J Fraser, Milone Michael C, Levine Bruce L, June Carl H: Chimeric antigen receptor-modified T cells for acute lymphoid leukemia. The New England journal of medicine 368(16): 1509-18, Apr 2013.

Le Gall J B, Milone M C, Waxman I M, Shaw L M, Harrison L, Duffy D, van de Ven C, Militano O, Geyer M B, Morris E, Bhatia M, Satwani P, George D, Garvin J H, Bradley M B, Schwartz J, Baxter-Lowe L A, Cairo M S: The pharmacokinetics and safety of twice daily i.v. BU during conditioning in pediatric allo-SCT recipients. Bone marrow transplantation 48(1): 19-25, Jan 2013.

Kunii Naoki, Zhao Yangbing, Jiang Shuguang, Liu Xiaojun, Scholler John, Balagopalan Lakshmi, Samelson Lawrence E, Milone Michael C, June Carl H: Enhanced function of redirected human T cells expressing linker for activation of T-cells that is resistant to ubiquitylation. Human gene therapy 24(1): 27-37, Jan 2013.

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Last updated: 11/25/2015
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