Michael C. Milone

faculty photo
Assistant Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
Staff Physician, Department of Pathology and Laboratory Medicine
Department: Pathology and Laboratory Medicine

Contact information
3400 Spruce Street 7103 Founders Pavilion
Philadelphia, PA 19104
Office: 215-662-6575
Fax: 215-662-7529
Graduate Group Affiliations
Education:
B.S (Chemical Biology)
Stevens Institute of Technology, 1993.
Ph.D (Experimental Pathology/ Immunology)
UMDNJ - Graduate School of Biomedical Sciences, 1999.
M.D. (Medical Doctor)
UMDNJ- New Jersey Medical School, 1999.
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Description of Research Expertise

Research in my laboratory aims toward improving cancer and transplantation therapy through the development of new immunotherapeutic modalities. Active research projects in my laboratory include:

1) Developing improved chimeric antigen receptors (CARs) for adoptive immunotherapy of cancer.

These projects are focused upon understanding the mechanisms of signal transduction of artificial chimeric antigen receptors through traditional biochemical studies along with high resolution dynamic imaging of artificial and natural T cell receptors. New CAR designs are also under development to permit more controlled activation of T cells.

2) Developing novel T cell stimulatory substrates based upon nano- and micro-fabricated surfaces for control of T cell proliferation and differentiation.

This project is part of a collaborative effort supported by the NIH Common Fund. Details of this project can be found at the center's website (www.mechanicalbiology.org) or the NIH common fund site (http://commonfund.nih.gov/nanomedicine/devcenters/mechanicalbiology.asp)

Description of Itmat Expertise

My laboratory is focused upon developing methods to use the immune system to treat cancer. Active research includes 1) exploring the mechanical properties of artificial adhesion substrates used in T cell culture and their influence on T cell biology, 2) development of artificial antigen receptors and signaling systems for control of T cell specificity

Selected Publications

Moon Edmund K, Wang Liang-Chuan, Dolfi Douglas V, Wilson Caleph B, Ranganathan Raghuveer, Sun Jing, Kapoor Veena, Scholler John, Puré Ellen, Milone Michael C, June Carl H, Riley James L, Wherry E John, Albelda Steven M: Multifactorial T-cell Hypofunction That Is Reversible Can Limit the Efficacy of Chimeric Antigen Receptor-Transduced Human T cells in Solid Tumors. Clinical cancer research : an official journal of the American Association for Cancer Research 20(16): 4262-73, Aug 2014.

Bashour Keenan T, Tsai Jones, Shen Keyue, Lee Joung-Hyun, Sun Eileen, Milone Michael C, Dustin Michael L, Kam Lance C: Crosstalk between CD3 and CD28 is spatially modulated by protein lateral mobility. Molecular and cellular biology Dec 2013.

Cardamone David, Milone Michael C, Glaser Laurel, Frey Noelle V, Kricka Larry J: Eltrombopag and serum of a different hue. Archives of pathology & laboratory medicine 137(9): 1175, Sep 2013.

Grupp Stephan A, Kalos Michael, Barrett David, Aplenc Richard, Porter David L, Rheingold Susan R, Teachey David T, Chew Anne, Hauck Bernd, Wright J Fraser, Milone Michael C, Levine Bruce L, June Carl H: Chimeric antigen receptor-modified T cells for acute lymphoid leukemia. The New England journal of medicine 368(16): 1509-18, Apr 2013.

LeGall J, Milone MC, Waxman I, Shaw LM, Harrison L, Duffy D, van de Ven C, Militano O, Geyer MB, Morris E, Bhatia M, Satwani P, George D, Garvin JH, Bradley MB, Schwartz J, Baxter-Lowe LA, Cairo MS.: The Pharmacokinetics and Safety of Twice Daily Intravenous Busulfan During Conditioning in Pediatric Allogeneic Stem Cell Transplantation Recipients. Bone Marrow Transplant. 48(1): 19-25, Jan 2013.

Kunii Naoki, Zhao Yangbing, Jiang Shuguang, Liu Xiaojun, Scholler John, Balagopalan Lakshmi, Samelson Lawrence E, Milone Michael C, June Carl H: Enhanced function of redirected human T cells expressing linker for activation of T-cells that is resistant to ubiquitylation. Human gene therapy 24(1): 27-37, Jan 2013.

O’Connor RS, Hao X, Shen K, Bashour K, Akimova T, Hancock WW, Kam LC and Milone MC: Substrate rigidity regulates human T cell activation and proliferation. J Immunol. 189(3): 1330-9, August 2012.

Babich A, Li S, O'Connor RS, Milone MC, Freedman BD, Burkhardt JK.: F-actin polymerization and retrograde flow drive sustained PLCγ1 signaling during T cell activation. J Cell Biol. 197(6): 775-787, June 2012.

Friend Kerri, Milone Michael C, Perrone Jeanmarie: Hyponatremia Associated With Levamisole-Adulterated Cocaine Use in Emergency Department Patients. Annals of emergency medicine Sep 2011.

Biggs M J P, Milone M C, Santos L C, Gondarenko A, Wind S J: High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates. Journal of the Royal Society, Interface / the Royal Society Jun 2011.

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Last updated: 09/02/2014
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