Description of Research Expertise

Tissue culture: establishment of primary human airway cultures and air liquid interface cell cultures, transient transfection for virus (lentivirus and AAV virus vector) production, analysis of transduced cells (FACS Scan/ PCR/ histology). Molecular biology: virology, DNA structure analysis, Northern and Western blots, in situ hybridisation, PCR. Animal-related work: minor survival surgeries (intratracheal administrations), gene delivery studies, preparation of tissues for histological analysis, luciferase gene expression imaging by the Xenogen imaging system, establishment and maintenance of CF mouse colonies, transepithelial potential difference measurements. Immunology: Ex vivo and cultured IFN-gamma ELISPOT assay and multi-colour ICCS assays.

Description of Other Expertise

Proficient in Word, Excel, Powerpoint, Photoshop & vectorNTI,

Description of Itmat Expertise

Dr. Limberis investigates gene therapy for lung diseases, including the development of viral-mediated gene delivery approaches as a potential therapy for diseases that include cystic fibrosis and a-1-antitrypsin deficiency.