Maria P. Limberis
Research Assistant Professor of Pathology and Laboratory Medicine
Department: Pathology and Laboratory Medicine
Contact information
125 South 31st street, Suite 2011, TRL
Philadelphia, PA 19103
Philadelphia, PA 19103
Office: 215-898-0226
Fax: 215-898-6588
Lab: 215-898-3123
Fax: 215-898-6588
Lab: 215-898-3123
Email:
limberis@mail.med.upenn.edu
limberis@mail.med.upenn.edu
Publications
Education:
B.Sc. Hons (Biochemistry)
Liverpool John Moores University, United Kingdom, 1997.
Ph.D. (Molecular Biology)
University of Adelaide, Australia, 2003.
Permanent linkB.Sc. Hons (Biochemistry)
Liverpool John Moores University, United Kingdom, 1997.
Ph.D. (Molecular Biology)
University of Adelaide, Australia, 2003.
Description of Research Expertise
Dr. Limberis investigates gene therapy for genetic lung diseases that include cystic fibrosis and alpha-1-antitrypsin deficiency. She is developing adeno-associated virus vectors and lentiviral vectors for the delivery of therapeutic genes to the affected airway epithelium. She also studies the immunological implications of lung-directed gene therapy with a specific emphasis on cellular immune activation to therapeutic transgene products. She is also developing AAV-based vaccines for airborne infectious diseases that include influenza.Description of Itmat Expertise
Dr. Limberis investigates gene therapy for genetic lung diseases that include cystic fibrosis and alpha-1-antitrypsin deficiency. She is developing adeno-associated virus vectors and lentiviral vectors for the delivery of therapeutic genes to the affected airway epithelium. She also studies the immunological implications of lung-directed gene therapy with a specific emphasis on cellular immune activation to therapeutic transgene products.Selected Publications
Huang WC, Wright AF, Roman AJ, Cideciyan AV, Manson FD, Gewaily DY, Schwartz SB, Sadigh S, Limberis MP, Bell P, Wilson JM, Swaroop A, Jacobson SG: RPGR-associated Retinal Degeneration in Human X-linked RP and a Murine Model. Invest Ophthalmol Vis Sci 53(9): 5594-608, Aug 2012.M.G. Davey, P.W. Zoltick, C.A. Todorow, M.P. Limberis, H.L. Hedrick, A.W. Flake.: Jaagsiekte sheep retrovirus pseudotyped lentiviral vector-mediated gene transfer to fetal ovine lung. Gene Therapy 2(19): 201-9, Feb 2012.
Aleman TS, Cideciyan AV, Aguirre GK, Huang WC, Mullins CL, Roman AJ, Sumaroka A, Olivares MB, Tsai FF, Schwartz SB, Vandenberghe LH, Limberis MP, Stone EM, Bell P, Wilson JM, Jacobson SG: Human CRB1-associated retinal degeneration: comparison with the rd8 Crb1-mutant mouse model. Invest Ophthalmol Vis Sci 52(9): 6898-910, Aug 2011.
Bell CL, Vandenberghe LH, Bell P, Limberis MP, Gao GP, Van Vliet K, Agbandje-McKenna M, Wilson JM: The AAV9 receptor and its modification to improve in vivo lung gene transfer in mice. J Clinical Investigation 121(6): 2427-2435, June 2011.
Zhang L, Limberis MP, Thompson CI, Antunes MA, Luongo C, Wilson JM,Collins PL, Pickles RJ: Alpha-Fetoprotein Gene Delivery to the Nasal Epithelium of Nonhuman Primates by Human Parainfluenza Virus Vectors. Human Gene Therapy 21(12): 1657-64, Dec 2010.
Limberis, MP, Bell CL, Heath J and Wilson JM: Activation of transgene-specific T cells following lentivirus-mediated gene delivery to mouse lung. Molecular Therapy 18(1): 143-50, Jan 2010.
David E Fein, Maria P Limberis, Sean F Maloney, Jack M Heath, James M Wilson and Scott L Diamond: Cationic Lipid Formulations Alter the In Vivo Tropism of AAV2/9 Vector in Lung. Molecular Therapy 17(12): 2078-87, Dec 2009.
Limberis, MP, Bell, CL and Wilson, JM.: Identification of the mouse firefly luciferase-specific T cell epitopes. Gene Therapy 16(3): 441-7, Mar 2009.
Yuhu Song, Howard Lou, Julie Boyer, Maria P. Limberis, Luk H. Vandenberghe, Neil Hackett, Philip Leopold, James M. Wilson, Ronald G. Crystal: Functional CFTR Expression in Cystic Fibrosis Airway Epithelial Cells by AAV6.2-mediated Segmental Trans-splicing. Human Gene Therapy 20(3): 267-81, Mar 2009.
Limberis MP, Vandenberghe LH, Zhang L, Pickles RJ, Wilson JM.: Transduction Efficiencies of Novel AAV Vectors in Mouse Airway Epithelium In Vivo and Human Ciliated Airway Epithelium In Vitro. Molecular Therapy 17(2): 294-301, Feb 2009.