Gary D. Wu

Associate Professor of Medicine

Department: Medicine

Contact information

415 Curie Boulevard
638A Clinical Research Building/6140
Philadelphia, PA 19104

Office: (215)898-0158

Email:
gdwu@mail.med.upenn.edu

Graduate Group Affiliations

Pharmacological Sciences

Publications

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Links
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Education
A.B. (Chemistry)
Cornell University, 1982.
M.D.
Northwestern University Medical School, 1986.
M.A. (Honorary)
University of Pennsylvania, 2002.

Permanent link

Description of Research Expertise

Research Program
Molecular regulation of gene expression in the colonic epithelium

Program Summary
Intestinal epithelial cells respond to inflammatory stimulation in several ways. First, intestinal epithelial cells can produce immune modulating substances such as cytokines and immunoreceptors. Second, intestinal inflammation leads to an alteration in the patterns of cell differentiation along the crypt-villus axis. Finally, chronic inflammatory diseases of intestinal tract lead to a markedly increased risk for the neoplastic transformation of the colonic epithelium. Using various intestinal cell culture systems and transgenic animal models, we are exploring the molecular mechanisms by which gene expression is regulated in the colonic epithelium. Target genes which are being studied in this regard include the proinflammatory cytokine, IL-8, the chloride/sulfate anion transporter, DRA, the nuclear hormone receptor, PPARgamma, and a novel gene expressed specifically in colonic crypt cells, RELM-beta.

Lab personnel:
Tanesha Cash-Mason, Research Technician - CHOP
Marie Hildebrandt, Graduate Student
Sue Keilbaugh, PhD, Technical Director, Molecular Biology Core
Mei Lun Wang, MD, Assistant Professor of Pediatrics - CHOP
Tracie Wong, MD, CHOP Fellow

Selected Publications

McVay L.D., Keilbaugh S.A., Wong T.M.H., Shin M.E., Kierstein S., Lehrke M., Lefterova M.I., Schifflett D.E., Barnes S.L., Cominelli F., Cohn S.M., Hecht G., Lazar M.A., Haczku A., Wu G.D. : Absence of Bacterially-Induced RELMbeta Reduces Injury in the DSS Model of Colitis. The Journal of Clinical Investigation 116: 2914-2923, 2006.

Wang M.L., Shin M., Artis D., Silberg D.G., Wu G.D. : Regulation of RELM/FIZZ isoform expression by Cdx2 in response to innate and adaptive immune stimulation in the intestine. AJP-Gastro 288(5): G1074-1083, May 2005.

Keilbaugh S.A., Shin M.E., McVay L., Boyko N., Artis D., Cebra J.J., Wu G.D. : Activation of RegIIIbeta/gamma and Interferon-gamma Expression in the Intestinal Tract of SCID Mice, an Innate Response to Bacterial Colonization of the Gut. Gut 54(5): 623-629, May 2005.

Artis D., Wang M.L., Keilbaugh S.A., He W., Brenes M., Swain G.P., Knight P.A., Donaldson D.D., Lazar M.A., Miller H.R.P., Schad G.A., Scott P., Wu G.D. : RELMbeta/FIZZ2 is a Novel Goblet Cell-Specific Immune Effector Molecule in the Gastrointestinal Tract. PNAS 101: 13596-600, 2004.

Artis D., Villarino A., Silverman M., He W., Thornton E., Mu S., Summer S., Covey T, Huang E., Yoshida H., Koretzky G, Goldschmidt M., Wu G.D., de Sauvage F., Millar H., Saris C., Scott P., Hunter C.A. : The IL-27R (WSX-1) inhibits innate and adaptive elements of intestinal helminth-induced T helper 2 responses. Journal of Immunology 173: 5626-5634, 2004.

He W., Wang M.L., Steppan C., Thurnheer M.C., Jiang H.Q., Cebra J.J., Lazar MA, Wu G.D. : Bacterial Colonization Leads to the Colonic Secretion of RELMbeta/FIZZ2, a Novel Goblet Cell-Specific Protein. Gastroenterology 125: 1388-1397, 2003.

ZaiFang Y., Cheng G., Wen X., Wu G.D., Lee W.T., Pleasure D. : Induction of GluR1 by tumor necrosis factor alpha, mediated by acid sphiugomyelinase and NFkappa B, sensitizes neurons to excitotoxic necrosis. Neurobiol. Dis. 11: 199-213, 2002.

Steppan C.M., Brown E.B., Wright C.M., Bhat S., Banerjee R., Dai C., Enders G., Silberg D., Wen X.M., Wu G.D., Lazar M.A. : A Novel Family of Tissue-Specific Resistin-Like Molecules. PNAS 98: 502-506, 2001.

Lewis J.D., Stein R.B., Deren J.J., Judge T.A., Furth E.E., Fogt F., Demissie E.J., Hurd L.B., Keilbaugh S.A., Lazar M.A., Lichtenstein G.R., Wu G.D. : An Open-Label Trial of the PPARgamma Ligand Rosiglitazone for Active Ulcerative Colitis. Am. J. Gastro. 96: 3323-3328, 2001.

Elliott C.L., Allport V.C., Loudon J.A.Z., Wu G.D., Bennett P.R. : Nuclear Factor-kappaB is Essential for Up-regulation of IL-8 Expression in Human Amnion and Cervical Epithelial Cells. Molecular Human Reproduction 7: 787-790, 2001.

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