c
2
12
18
28
12
12
1a
14
e
12
16
a
a
2
2
a
20
32
1a
2a
19
Faculty
61 16
19
1
49
2
2
1a
32
34
1b
1d
18
38
66
1d
2 29
1d
25
Harry Ischiropoulos, Ph.D.
78
66
Research Professor of Pediatrics (Neonatology & Newborn Services)
7
61
Department: Pediatrics
4
1
23
1f
Graduate Group Affiliations
8
b
-
6a
- Pharmacology 64
- Neuroscience e
1d
46
Contact information
51
4
3
3
1d
51
Children's Hospital of Philadelphia
4a 416B Abramson Pediatric Research Center
Philadelphia, PA 19104
26
4a 416B Abramson Pediatric Research Center
Philadelphia, PA 19104
2e
Office: 215-590-5320
3e Lab: 215-590-5319
18
9f
12
3e Lab: 215-590-5319
18
18
Publications
23 a
3
2
4
b
1f
23 a
13
Education:
21 9 B.S. 16 (Chemistry) c
27 Wagner College, 1984.
21 9 M.S. 16 (Pathology) c
31 New York Medical College, 1987.
21 a Ph.D. 23 (Experimental Pathology) c
31 New York Medical College, 1990.
c
3
27
21 9 B.S. 16 (Chemistry) c
27 Wagner College, 1984.
21 9 M.S. 16 (Pathology) c
31 New York Medical College, 1987.
21 a Ph.D. 23 (Experimental Pathology) c
31 New York Medical College, 1990.
c
Links
9e Search PubMed for articles
91 CHOP Research Institute faculty web page
48 Pharmacological Sciences graduate group faculty webpage.
46 Neuroscience graduate group faculty webpage.
c
5
3
3
90
Permanent link9e Search PubMed for articles
91 CHOP Research Institute faculty web page
48 Pharmacological Sciences graduate group faculty webpage.
46 Neuroscience graduate group faculty webpage.
c
2 29
21
1e
1d
24
5e
e1 Biological chemistry of nitric oxide in cardiovascular and neuronal systems. Oxidative processes in protein aggregation and neurodegeneration. Mass spectrometry-based discovery and validation of disease biomarkers.
8
11 KEY WORDS
a2 Biological chemistry of nitric oxide; oxidative processes and stress; protein aggregation and neurodegeneration; post-translational protein modifications.
8
1b RESEARCH TECHNIQUES
8f Biochemical analysis of post-translational modifications; liquid chromatography-mass spectrometry; proteomics; nitric oxide detection.
8
18 RESEARCH SUMMARY
e4 We investigate the biological chemistry and molecular mechanisms of nitric oxide signaling. Nitric oxide is a free radical that mediates blood flow and many other physiological responses within every major organ system.
a
18e Currently mass spectrometry-based proteomic data in conjunction with structure-function analysis explore the biochemical and biophysical specificity of two nitric oxide-mediated post-translational modifications cysteine S-nitrosation and tyrosine nitration. Studies explore the consequences of these modifications on protein function in metabolic processes and mitochondrial bioenergetics.
8
139 We are also generating inventories of mouse brain proteomes, phosphoproteomes, S-nitrosoproteomes and secretomes. These inventories are used to create 3D-functional landscapes of the mouse brain extracellular space and to identify signaling pathways that influence neuron physiology and neurodegeneration.
e 29
27
Description of Research Expertise
23 RESEARCH INTERESTSe1 Biological chemistry of nitric oxide in cardiovascular and neuronal systems. Oxidative processes in protein aggregation and neurodegeneration. Mass spectrometry-based discovery and validation of disease biomarkers.
8
11 KEY WORDS
a2 Biological chemistry of nitric oxide; oxidative processes and stress; protein aggregation and neurodegeneration; post-translational protein modifications.
8
1b RESEARCH TECHNIQUES
8f Biochemical analysis of post-translational modifications; liquid chromatography-mass spectrometry; proteomics; nitric oxide detection.
8
18 RESEARCH SUMMARY
e4 We investigate the biological chemistry and molecular mechanisms of nitric oxide signaling. Nitric oxide is a free radical that mediates blood flow and many other physiological responses within every major organ system.
a
18e Currently mass spectrometry-based proteomic data in conjunction with structure-function analysis explore the biochemical and biophysical specificity of two nitric oxide-mediated post-translational modifications cysteine S-nitrosation and tyrosine nitration. Studies explore the consequences of these modifications on protein function in metabolic processes and mitochondrial bioenergetics.
8
139 We are also generating inventories of mouse brain proteomes, phosphoproteomes, S-nitrosoproteomes and secretomes. These inventories are used to create 3D-functional landscapes of the mouse brain extracellular space and to identify signaling pathways that influence neuron physiology and neurodegeneration.
e 29
23
14d Thomson L, Tenopoulou M, Lightfoot R, Tsika E, Parastatidis I, Martinez M, Greco TM, Doulias PT, Wu Y, Tang WHW, Hazen SL, Ischiropoulos H: Immunoglobulins against tyrosine-nitrated epitopes in coronary artery disease. Circulation 126(20): 2392-2401, Nov 2012.
171 Doulias PT, Greene JL, Greco TM, Tenopoulou M, Seeholzer SH, Dunbrack RL, Ischiropoulos H: Structural profiling of endogenous S-nitrosocysteine residues reveals unique features that accommodate diverse mechanisms for protein S-nitrosylation. Proc Natl Acad Sci U S A 107(39): 6958-6963, Sep 2010.
124 Greco TM, Seeholzer SH, Mak A, Spruce L, Ischiropoulos H: Quantitative mass spectrometry-based proteomics reveals the dynamic range of primary mouse astrocyte protein secretion. J Proteome Res 9(5): 2764-2774, May 2010.
157 Tsika E, Moysidou M, Guo J, Cushman M, Gannon P, Sandaltzopoulos R, Giasson BI, Krainc D, Ischiropoulos H, Mazzulli JR: Distinct region-specific alpha-synuclein oligomers in A53T transgenic mice: implications for neurodegeneration. J Neurosci 30(9): 3409-3418, Mar 2010.
15e Parastatidis I, Thomson L, Burke A, Chernysh I, Nagaswami C, Visser J, Stamer S, Liebler DC, Koliakos G, Heijnen HFG, Fitzgerald GA, Weisel JW, Ischiropoulos H: Fibrinogen beta-chain tyrosine nitration is a prothrombotic risk factor. J Biol Chem 283(49): 33846-33853, Dec 2008.
2c
7
1d
1f
Selected Publications
10f Doulias PT, Tenopoulou M, Greene JL, Raju K, Ischiropoulos H: Nitric oxide regulates mitochondrial fatty acid metabolism through reversible protein S-nitrosylation. Sci Signal 6(256): rs1, Jan 2013.14d Thomson L, Tenopoulou M, Lightfoot R, Tsika E, Parastatidis I, Martinez M, Greco TM, Doulias PT, Wu Y, Tang WHW, Hazen SL, Ischiropoulos H: Immunoglobulins against tyrosine-nitrated epitopes in coronary artery disease. Circulation 126(20): 2392-2401, Nov 2012.
171 Doulias PT, Greene JL, Greco TM, Tenopoulou M, Seeholzer SH, Dunbrack RL, Ischiropoulos H: Structural profiling of endogenous S-nitrosocysteine residues reveals unique features that accommodate diverse mechanisms for protein S-nitrosylation. Proc Natl Acad Sci U S A 107(39): 6958-6963, Sep 2010.
124 Greco TM, Seeholzer SH, Mak A, Spruce L, Ischiropoulos H: Quantitative mass spectrometry-based proteomics reveals the dynamic range of primary mouse astrocyte protein secretion. J Proteome Res 9(5): 2764-2774, May 2010.
157 Tsika E, Moysidou M, Guo J, Cushman M, Gannon P, Sandaltzopoulos R, Giasson BI, Krainc D, Ischiropoulos H, Mazzulli JR: Distinct region-specific alpha-synuclein oligomers in A53T transgenic mice: implications for neurodegeneration. J Neurosci 30(9): 3409-3418, Mar 2010.
15e Parastatidis I, Thomson L, Burke A, Chernysh I, Nagaswami C, Visser J, Stamer S, Liebler DC, Koliakos G, Heijnen HFG, Fitzgerald GA, Weisel JW, Ischiropoulos H: Fibrinogen beta-chain tyrosine nitration is a prothrombotic risk factor. J Biol Chem 283(49): 33846-33853, Dec 2008.
2c
4d
22
22
7
10
a
a
2
2
19
18
10
22
10
11
c
5b © The Trustees of the University of Pennsylvania | Site best viewed a in a supported browser. | Site Design: 57 PMACS Web Team. 3 22
10