Greg J. Bashaw, Ph.D.

faculty photo
Professor of Neuroscience
Department: Neuroscience
Graduate Group Affiliations

Contact information
Department of Neuroscience
135A Clinical Research Building
415 Curie Blvd.
Philadelphia, PA 19104
Office: 215 898-0829
Fax: 215 573-7601
Lab: 215-898-0854
Education:
B.A. (Biology)
Brown University, 1990.
Ph.D. (Biological Sciences)
Stanford University, Mentor: Bruce Baker, 1997.
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Description of Research Expertise

Research Interests
- Molecular mechanisms of axon growth and guidance during nervous system development.
- How axon guidance receptors specify attractive and repulsive signals and transmit these signals to the navigating growth cone.
- Formation of Neural Circuits at the Midline
- Transcriptional Regulation of Neuronal Morphogenesis
- Generation of Neuronal Diversity

Key words: Axon guidance, developmental neuroscience, Slit, Robo, Netrin.

Description of Research
How axons in the developing nervous system successfully navigate to their correct targets is a fundamental problem in neurobiology. Understanding the mechanisms that mediate axon guidance will give important insight into how the nervous system is correctly wired during development and may have implications for therapeutic approaches to developmental brain disorders and nerve regeneration. Achieving this understanding will require unraveling the molecular logic that ensures the proper expression and localization of axon guidance cues and receptors, and elucidating the signaling events that regulate the growth cone cytoskeleton in response to guidance receptor activation.

The Slit ligand and Roundabout (Robo) receptors, and the Netrin ligand and DCC/UNC5 receptors are two important evolutionary conserved ligand/receptor systems that contribute to proper connectivity in both the vertebrate and invertebrate nervous systems. These molecules are also known to influence neuronal and mesodermal cell migration, suggesting that determining their function may have broad implications for understanding diseases of nervous system development, many of which have their root in defective cell migration and/or axon guidance. The research in my laboratory addresses the dynamics of axon guidance receptor expression and signaling, and exploits the powerful genetic and molecular approaches available in Drosophila.

Rotation Projects
A range of projects relating to axon guidance receptor regulation and signaling, employing a broad range of techniques in genetics, molecular biology and biochemistry. For specific projects contact Dr. Bashaw.

Lab personnel:

Elise Arbeille, Ph.D., Post-doctoral Fellow
Rebecca Chance, Neuroscience Graduate Student
Alexandra Neuhaus-Follini, Neuroscience Graduate Student
Melissa Hernandez, Neuroscience Graduate Student
Celine Santiago, CAMB Graduate Student
Samantha Russell, CAMB Graduate Student
Nick O'Neill, Research Specialist
Ethan Rohrbach, Undergraduate Researcher
Elizabeth Curry, High School Teacher for NSF outreach program

Selected Publications

Santiago, C., Labrador, J.P., and Bashaw, G.J.: The homeodomain transcription factor Hb9 controls axon guidance in Drosophila through the regulation of Robo receptors. Cell Reports 7(1): 153-165. April 2014.

Zarin AA, Asadzadeh J, Hokamp K, McCartney D, Yang L, Bashaw GJ, Labrador JP.: A Transcription Factor Network Coordinates Attraction, Repulsion, and Adhesion Combinatorially to Control Motor Axon Pathway Selection. Neuron 81(6): 1297-1311, March 2014.

O'Donnell MP, Bashaw GJ.: Distinct functional domains of the Abelson tyrosine kinase control axon guidance responses to Netrin and Slit to regulate the assembly of neural circuits. Development 140(13): 2724-33, July 2013.

Michael O'Donnell and Greg J. Bashaw: Src inhibits midline axon crossing independent of Frazzled/DCC receptor tyrosine phosphorylation. Journal of Neuroscience 33(1): 305-314, January 2013.

Timothy A. Evans and Greg J. Bashaw: Slit/Robo-mediated axon guidance in Tribolium and Drosophila: Divergent genetic programs build insect nervous systems. Developmental Biology 363 : 266–278, March 2012.

Coleman Hope A, Labrador Juan-Pablo, Chance Rebecca K, Bashaw Greg J: The Adam family metalloprotease Kuzbanian regulates the cleavage of the roundabout receptor to control axon repulsion at the midline. Development 137(14): 2417-26, Jul 2010.

Timothy A. Evans and Greg J. Bashaw: Functional Diversity of Robo Receptor Immunoglobulin Domains Promotes Distinct Axon Guidance Decisions. Current Biology 20(6), March 2010.

Yang Long, Garbe David S, Bashaw Greg J: A frazzled/DCC-dependent transcriptional switch regulates midline axon guidance. Science 324(5929): 944-7, May 2009.

Garbe, D.S., O’Donnell, M., and Bashaw, G.J.: Cytoplasmic Domain Requirements for Frazzled-mediated Attractive Axon Turning at the Drosophila Midline. Development 134: 4315-4324. December 2007.

David S. Garbe and Greg J. Bashaw: Independent Functions of Slit-Robo Repulsion and Netrin-Frazzled Attraction Regulate Axon Crossing at the Midline in Drosophila. Journal of Neuroscience 27(13): 3584-3592, March 2007.

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Last updated: 05/11/2015
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