Gerd A. Blobel, MD, PhD
3615 Civic Center Blvd.
Philadelphia, PA 19104
Fax: (215) 590-4834
Lab: (215) 590-4461
Description of Research ExpertiseResearch Interests
higher order chromatin structure, gene expression, epigenetics, globin genes, sickle cell disease, therapeutics
Description of Research
We study how genetic regulatory elements are organized spatially in the nucleus and how transcription programs and chromatin architecture are organized throughout the cell cycle to maintain lineage identity. A major effort in the lab is directed towards understanding the regulation of globin gene expression and developing approaches to perturb globin gene expression to ameliorate sickle cell disease. Our work bridges basic science with preclinical studies. For our studies we combine molecular, genomic, biochemical, and imaging approaches with studies in normal and gene targeted mice.
Lab Rotation Projects:
For information about rotation projects please contact me directly.
Carly Geronimo, postdoctoral fellow
Peng Huang, postdoctoral fellow
Anna Huang, technician
Eugene Khandros, postdoctoral fellow
Maryanne Kihiu, technician
Xianjiang Lan, postdoctoral fellow
Jessica Lam, combined degree student
Jing Luan, combined degree student
Scott Peslak, postdoctoral fellow
Kunhua Qin, postdoctoral fellow
Malini Sharma, technician
Camille Syrett,postdoctoral fellow
Marit Vermunt, postdoctoral fellow
Aoi Wakabayashi, graduate student
Hongxin Wang, technician/lab manager
Jennifer Yano, technician
Adam Zhang, postdoctoral fellow
Di Zhang, combined degree student
Jim Zhang, bioinformatician
Selected PublicationsZhang Haoyue, Emerson Daniel J, Gilgenast Thomas G, Titus Katelyn R, Lan Yemin, Huang Peng, Zhang Di, Wang Hongxin, Keller Cheryl A, Giardine Belinda, Hardison Ross C, Phillips-Cremins Jennifer E, Blobel Gerd A: Chromatin structure dynamics during the mitosis-to-G1 phase transition. Nature 576(7785): 158-162, 12 2019.
Behera Vivek, Stonestrom Aaron J, Hamagami Nicole, Hsiung Chris C, Keller Cheryl A, Giardine Belinda, Sidoli Simone, Yuan Zuo-Fei, Bhanu Natarajan V, Werner Michael T, Wang Hongxin, Garcia Benjamin A, Hardison Ross C, Blobel Gerd A: Interrogating Histone Acetylation and BRD4 as Mitotic Bookmarks of Transcription. Cell reports 27(2): 400-415.e5, Apr 2019.
Bartman CR, Hamagami N, Keller CA, Giardine B, Hardison RC, Blobel GA, Raj A: Transcriptional Burst Initiation and Polymerase Pause Release Are Key Control Points of Transcriptional Regulation. Molecular Cell 2019.
Behera V, Evans P, Face CJ, Hamagami N, Sankaranarayanan L, Keller CA, Giardine B, Tan K, Hardison RC, Shi J, Blobel GA: Exploiting genetic variation to uncover rules of transcription factor binding and chromatin accessibility. Nature Communications 2018.
Grevet JD, Lan X, Hamagami N, Edwards CR, Sankaranarayanan L, Ji X, Bhardwaj SK, Face CJ, Posocco DF, Abdulmalik O, Keller CA, Giardine B, Sidoli S, Garcia BA, Chou ST, Liebhaber SA, Hardison RC, Shi J, and Blobel GA: Domain-focused CRISPR-screen identifies HRI as a fetal hemoglobin regulator in human erythroid cells. Science 2018.
Hsu SC, Gilgenast TG, Bartman CR, Edwards CR, Stonestrom AJ, Huang P, Emerson DJ, Evans P, Werner MT, Keller CA, Giardine B, Hardison RC, Raj A, Phillips-Cremins JE, Blobel GA: The BET Protein BRD2 Cooperates with CTCF to Enforce Transcriptional and Architectural Boundaries. Molecular Cell 2017.
Huang P, Keller CA, Giardine B, Grevet JD, Davies JOJ, Hughes JR, Kurita R, Nakamura Y, Hardison RC, Blobel GA.: Comparative analysis of three-dimensional chromosomal architecture identifies a novel fetal hemoglobin regulatory element. Genes and Development 2017.
Deng W, Rupon JW, Krivega I, Breda L, Motta I, Jahn KS, Reik A, Gregory PD, Rivella S, Dean A, Blobel GA: Reactivation of developmentally silenced globin genes by forced chromatin looping. Cell 2014.
Kadauke S, Udugama MI, Pawlicki JM, Achtman JC,Jain DP, Cheng Y, Hardison RC, and Blobel GA: Tissue-specific Mitotic Bookmarking by Hematopoietic Transcription Factor GATA1. Cell 2012.
Deng W, Lee J, Wang H, Miller J, Reik A, Gregory PD, Dean A, Blobel GA: Controlling long-range genomic interactions at a native locus by targeted tethering of a looping factor. Cell 2012.