Grzegorz Rebowski, Ph.D.

Publications

Barrie KR, Rebowski G, Dominguez R (2024) Mechanism of Actin Filament Severing and Capping by Gelsolin. Nat Struct Mol Biol in press (see it in BioRxiv)

Xu M, Rutkowski DM, Rebowski G, Boczkowska M, Pollard LW, Dominguez R, Vavylonis D, Ostap EM (2024) Myosin-I Synergizes with Arp2/3 Complex to Enhance Pushing Forces of Branched Actin Networks. Sci Adv 10. DOI: 10.1126/sciadv.ado5788

Fregoso FE, Boczkowska M, Rebowski G, Carman PJ, Van Eeuwen T, Dominguez R (2023) Mechanism of synergistic activation of Arp2/3 complex by cortactin and WASP-family proteins. Nat Commun 14. DOI: 10.1038/s41467-023-42229-y

Van Eeuwen T, Boczkowska M, Rebowski G, Carman PJ, Fregoso FE, Dominguez R (2023) Transition State of Arp2/3 Complex Activation by Actin-Bound Dimeric Nucleation-Promoting Factor. PNAS 120. DOI: 10.1073/pnas.2306165120

Carman PJ, Barrie KR, Rebowski G, Dominguez R (2023) Structures of the free and capped ends of the actin filament. Science.
DOI: 10.1126/science.adg6812
Highlighted in Penn Today and in Faculty Opinions

Carman PJ, Rebowski G, Dominguez R, Alqassim SS (2023) Single particle cryo-EM analysis of Rickettsia conorii Sca2 reveals a formin-like core.
J Struct Biol. DOI: 10.1016/j.jsb.2023.107960

Pollard LW, Coscia SM, Rebowski G, Palmer NJ, Holzbaur ELF, Dominguez R , Ostap EM (2023) Ensembles of human myosin-19 bound to calmodulin and regulatory light chain RLC12B drive multi-micron transport. J Biol Chem. DOI: 10.1016/j.jbc.2023.102906

Ceron RH, Carman PJ, Rebowski G, Boczkowska M, Heuckeroth RO, Dominguez R (2022) A solution to the long-standing problem of actin expression and purification. PNAS 119(41). DOI: 10.1073/pnas.2209150119

Fregoso FE, Van Eeuwen T, Simanov G, Rebowski G, Boczkowska M, Zimmet A, Gautreau AM, Dominguez R (2022) Molecular mechanism of Arp2/3 complex inhibition by Arpin. Nat Commun 13:628. DOI: 10.1038/s41467-022-28112-2

Zimmet A, Van Eeuwen T, Boczkowska M, Rebowski G, Murakami K, Dominguez R (2020) Cryo-EM Structure of NPF-Bound Human Arp2/3 Complex and Activation Mechanism. Science Advances, 6(23):eaaz7651. DOI: 10.1126/sciadv.aaz7651

Rebowski G, Boczkowska M, Drazic A, Ree R, Goris M, Arnesen T, Dominguez R (2020) Mechanism of actin N-terminal acetylation. Science Advances 6(15):eaay8793. DOI: 10.1126/sciadv.aay8793

Drazic A, Aksnes H, Marie M, Boczkowska M, Varland S, Timmerman E, Foyn H, Glomnes N, Rebowski G, Impens F, Gevaert K, Dominguez R, Arnesen T (2018) NAA80 is actin’s N-terminal acetyltransferase and regulates cytoskeleton assembly and cell motility. PNAS 115:4399-4404. doi: 10.1073/pnas.1718336115

Boczkowska M, Yurtsever Z, Rebowski G, Eck MJ, Dominguez R (2017) Crystal structure of leiomodin 2 in complex with actin: A structural and functional reexamination Biophys J 113:889-899 (New & Notable by Thomas Pollard in Biophys J 113:762-764)

Boczkowska M, Rebowski G, Dominguez R (2015) The chalenges of polydisperse SAXS data analysis: Two SAXS studies of PICK1 produce different structural models. Stucture 23:1967-1968. DOI:10.1016/j.str.2015.10.007

Boczkowska M, Rebowski G, Kremneva E, Lappalainen P, Dominguez R (2015) How Leiomodin and Tropomodulin use a common fold for different actin assembly functions. Nature Commun 6:8314. DOI:10.1038/ncomms9314

Madasu Y, Yang C, Boczkowska M, Bethoney KA, Zwolak A, Rebowski G, Svitkina T, and Dominguez R (2015) PICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner. Mol. Biol. Cell E14-10-1448. [Epub ahead of print]

Boczkowska M, Rebowski G*, Kast DJ, Dominguez R (2014) Structural analysis of the transitional state of Arp2/3 complex activation by two actin-bound WCAs. Nature Commun 5:3308. DOI:10.1038/ncomms4308

Boczkowska M, Rebowski G, Dominguez R (2013) GMF interacts with Arp2/3 complex in a nucleotide state-dependent manner. J Biol Chem 288:25683-25688

Jensen MH, Morris EJ, Huang R, Rebowski G, Dominguez R, Weitz DA, Moore JR, Wang CL (2012) The conformational state of actin filaments regulates branching by actin-related protein 2/3 (Arp2/3) complex. J Biol Chem 287:31447-31453

Namgoong S, Boczkowska M, Glista MJ, Winkelman JD, Rebowski G, Kovar DR, Dominguez R (2011) Mechanism of actin filament nucleation by Vibrio VopL and implications for tandem-W domain nucleation. Nature Struct Mol Biol 18: 1060-1067

Jansen S, Collins A, Yang C, Rebowski G, Svitkina T*, Dominguez R* (2011) Mechanism of Actin Filament Bundling by Fascin. J Biol Chem 268: 30087-30096

Pykalainen A, Boczkowska M, Zhao H, Saarikangas J, Rebowski G, Jansen M, Hakanen J, Koskela E, Peranen J, Vihinen H, Jokitalo E, Salminen M, Ikonen E, Dominguez R*, Lappalainen P* (2011) Pinkbar is an epithelial-specific BAR domain protein that generates planar membrane structures. Nature Struct Mol Biol 18: 902-907

Rebowski G, Namgoong S, Boczkowska M, Leavis P, Navaza J, Dominguez R (2010) Structure of a Longitudinal Actin Dimer Assembled by Tandem W Domains - Implications for Actin Filament Nucleation. J Mol Biol 403:11-23

Rebowski G.,* Boczkowska M.,* Hayes D.B., Guo L., Irving T.C. & Dominguez R. (2008). X-ray scattering study of actin polymerization nuclei assembled by tandem Wdomains. PNAS 105:10785-10790.

Boczkowska M*, Rebowski G*, Petoukhov MV, Hayes DB, Svergun DI, Dominguez R. X-ray scattering study of activated Arp2/3 complex with bound actin-WCA. Structure. 2008 16:695-704.

Chereau D, Boczkowska M, Skwarek-Maruszewska A, Fujiwara I, Hayes DB, Rebowski G, Lappalainen P, Pollard TD, Dominguez R. (2008). Leiomodin is an actin filament nucleator in muscle cells. Science., 320:239-243.

Ferron F, Rebowski G, Lee SH, Dominguez R. Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena/VASP. EMBO J. 2007 26:4597-4606.

Lee SH, Hayes DB, Rebowski G, Tardieux I, Dominguez R. Toxofilin from Toxoplasma gondii forms a ternary complex with an antiparallel actin dimer. PNAS 2007 104:16122-16127.

Terrak M*, Rebowski G*, Lu RC, Grabarek Z, Dominguez R. Structure of the light chain-binding domain of myosin V. PNAS, 102:12718-12723.

Nawrot B, Antoszczyk S, Maszewska M, Rebowski G, Kuwabara T, Warashina M, Taira K, Stec WJ, (2002). Modulation of beta-secretase gene expression by action of catalytic nucleic acids. Nucleic Acids Res Suppl., (2):105-6.

Niewiarowski W, Gendaszewska E, Rebowski G, Wójcik M, Mikołajczyk B, Goss W, Soszyński M, Bartosz G. (2000). Multidrug resistance-associated protein--reduction of expression in human leukaemia cells by antisense phosphorothioate olignucleotides. Acta Biochim Pol, 47(4):1183-8.

Rebowski G, Wójcik M, Boczkowska M, Gendaszewska E, Soszyński M, Bartosz G, Niewiarowski W, (2001). Antisense hairpin loop oligonucleotides as inhibitors of expression of multidrug resistance-associated protein 1: their stability in fetal calf serum and human plasma. Acta Biochim Pol, 48(4):1061-76.

Kanagaratnam R, Misiura K, Rebowski G, Ramasamy R, (1998). Malaria merozoite surface protein antisense oligodeoxynucleotides lack antisense activity but function as polyanions to inhibit red cell invasion. Int J Biochem Cell Biol, 30(9):979-85.

Zielińska E, Niewiarowski W, Bodalski J, Rebowski G, Skretkowicz J, Mianowska K, Sekulska M, (1998). Genotyping of the arylamine N-acetyltransferase polymorphism in the prediction of idiosyncratic reactions to trimethoprim-sulfamethoxazole in infants. Pharm World Sci, 20(3):123-30.

Zielińska E, Niewiarowski W, Bodalski J, Stańczyk A, Bolanowski W, Rebowski G, (1997). Arylamine N-acetyltransferase (NAT2) gene mutations in children with allergic diseases. Clin Pharmacol Ther, Dec;62(6):635-42.

Ramasamy R, Kanagaratnam R, Misiura K, Rebowski G, Amerakoon R, Stec WJ, (1996) Anti-sense oligodeoxynucleoside phosphorothioates nonspecifically inhibit invasion of red blood cells by malaria parasites. Biochem. Biophys. Res. Commun, 218:930-933.