Roberto Dominguez, Ph.D.

Professor of Physiology

Ph.D. 1996 Institut Pasteur and Université Paris-Sud. Crystallography of Biological Macromolecules and Biochemistry

E-mail: droberto at pennmedicine . upenn . edu
Phone: (215) 573-4559



Kast DJ, Yang C, Disanza A, Boczkowska M, Madasu Y, Scita G, Svitkina T and Dominguez R (2014) Molecular mechanism of IRSp53 regulation by autoinhibition and activation by Cdc42. Nature Struct Mol Biol. doi: 10.1038/nsmb.2781. [Epub ahead of print]

Boczkowska M, Rebowski G, Kast DJ, Dominguez R (2014) Structural analysis of the transitional state of Arp2/3 complex activation by two actin-bound WCAs. Nature Commun 5:3308. DOI:10.1038/ncomms4308

Shuman H, Greenberg MJ, Zwolak A, Lin T, Sindelar CV, Dominguez R, Ostap EM (2014) A vertebrate myosin-I structure reveals unique insights into myosin mechanochemical tuning. PNAS 111:2116-2121

Turegun B, Kast D, Dominguez R (2013) Subunit Rtt102 controls the conformation of the Arp7/9 heterodimer and its interactions with nucleotide and the catalytic subunit of SWI/SNF remodelers. J Biol Chem 288:35758-35768

Zwolak A, Yang C, Feeser EA, Ostap EM, Svitkina T, Dominguez R (2013) CARMIL leading edge localization depends on a non-canonical PH domain and dimerization. Nature Commun 4:2523. DOI:10.1038/ncomms3523

Disanza A, Bisi S, Winterhoff M, Milanesi F, Ushakov DS, Kast D, Marighetti P, Romet-Lemonne G, Müller HM, Nickel W, Linkner J, Waterschoot D, Ampè C, Cortellino S, Palamidessi A, Dominguez R, Carlier MF, Faix J, Scita G (2013) Cdc42 switches IRSp53 from inhibition of actin growth to elongation by clustering of VASP. EMBO J 32:2735-2750

Boczkowska M, Rebowski G, Dominguez R (2013) GMF interacts with Arp2/3 complex in a nucleotide state-dependent manner. J Biol Chem 288:25683-25688

Madasu Y, Suarez C, Kast DJ, Kovar DR and Dominguez R (2013) Rickettsia Sca2 has evolved formin-like activity through a different molecular mechanism. PNAS 110:E2677-E2686

Kallenbach NR & Dominguez R (2012) Dystrophin's tandem calponin-homology domains: Is the case closed? Biophys J 103:1818-181

Jensen MH, Morris EJ, Huang R, Rebowski G, Dominguez R, Weitz DA, Moore JR, Wang CL (2012) The conformational state of actin filaments regulates branching by actin-related protein 2/3 (Arp2/3) complex. J Biol Chem 287:31447-31453

Rao JN, Rivera-Santiago R, Li W, Lehman R, Dominguez R (2012) Structural analysis of Smooth muscle tropomyosin alpha and beta isoforms. J Biol Chem 287:3167-3174

Dominguez R & Namgoong S (2012) Chapter 4.4: Actin Filament Nucleation and Elongation. Egelman E (Ed) In Comprehensive Biophysics. Elsevier

Namgoong S, Boczkowska M, Glista MJ, Winkelman JD, Rebowski G, Kovar DR, Dominguez R (2011) Mechanism of actin filament nucleation by Vibrio VopL and implications for tandem-W domain nucleation. Nature Struct Mol Biol 18:1060-1067

Jansen S, Collins A, Yang C, Rebowski G, Svitkina T*, Dominguez R* (2011) Mechanism of Actin Filament Bundling by Fascin. J Biol Chem 268:30087-30096

Pykalainen A, Boczkowska M, Zhao H, Saarikangas J, Rebowski G, Jansen M, Hakanen J, Koskela E, Peranen J, Vihinen H, Jokitalo E, Salminen M, Ikonen E, Dominguez R*, Lappalainen P* (2011) Pinkbar is an epithelial-specific BAR domain protein that generates planar membrane structures. Nature Struct Mol Biol 18:902-907

Kast D & Dominguez R (2011) Arp you ready for actin in the nucleus? EMBO J 30:2097-2098

Dominguez R (2011) Tropomyosin: the gatekeeper's view of the actin filament revealed. Biophys J 100:797-798

Dominguez R & Holmes KC (2011) Actin Structure And Function. Annu Rev Biophys 40:169-186

Gorelik R, Yang C, Kameswaran V, Dominguez R, Svitkina T (2011) Mechanisms of plasma membrane targeting of formin mDia2 through its amino terminal domains. Mol Biol Cell 22:189-201

Rebowski G, Namgoong S, Boczkowska M, Leavis P, Navaza J, Dominguez R (2010) Structure of a Longitudinal Actin Dimer Assembled by Tandem W Domains - Implications for Actin Filament Nucleation. J Mol Biol 403:11-23

Dominguez R (2010) The WASP-Homology 2 Domain and Cytoskeleton Assembly. In Actin-based Motility: Cellular, Molecular and Physical Aspects Marie-France Carlier, Editor Spinger Dordrecht Heidelberg London New York 2010

Skwarek-Maruszewska A, Boczkowska M, Zajac A, Kremneva E, Svitkina T, Dominguez R*, Lappalainen P* (2010) Different Localizations and Cellular Behaviors of Leiomodin and Tropomodulin in Mature Cardiomyocyte Sarcomeres. Mol Biol Cell 21:3352-3361

Lee SH & Dominguez R (2010) Regulation of actin cytoskeleton dynamics in cells. Mol Cells 29:311-325

Baek K, Knodler A, Lee SH, Zhang X, Orlando K, Zhang J, Foskett TJ, Guo W, Dominguez R (2010) Structure-Function Study of the N-terminal Domain of Exocyst Subunit Sec3  J Biol Chem 285:10424-10433

Dominguez R (2010) Structural insights into de novo actin polymerization. Curr Opin Struct Biol 20:217-225

Kim HR, Graceffa P, Ferron F, Gallant C, Boczkowska M, Dominguez R, Morgan KG (2010) Actin Polymerization in Differentiated Vascular Smooth Muscle Cells Requires Vasodilator-Stimulated Phosphoprotein (VASP).  Am J Physiol Cell Physiol 298:C559-C571

Dominguez R (2009) Actin filament nucleation and elongation factors — Structure-function relationships. Crit Rev Biochem Mol Biol 44:351-366

Shvetsov A, Berkane E, Chereau D, Dominguez R, Reisler E (2009) The actin-binding domain of cortactin is dynamic and unstructed and affects lateral and longitudinal contacts in F-actin. Cell Motil Cytoskeleton 66:90-98