Beckman Center for Cryo-Electron Microscopy

New User

All New Users are required to email for a consultation (cryo-em@lists.upenn.edu). Once you send the email with your request, we will get back to you about the project's feasibility, pricing, and goals.

Cryo-EM Screening

We provide both shorter Cryo-EM Screening sessions (shorter than 6 hours) as well as longer than 6–12 hour Cryo-EM sample screening sessions. The time slots will be awarded on a first-come, first-served basis. Users who wish to submit grids for screening should get in line by sending an email to cryo-em@lists.upenn.edu and expect a wait time of 1-2 weeks (this may change based on demand).

The price for 6 hours of screening is $250 (plus $18 per grid for the clip rings) and users can expect to screen 4-6 grids in a 6-hour session. 

The shorter screening sessions that are less than 6 hours of Microscope time are charged @ $50/hr

High-Res Data collection

We provide 24-72 hour sessions for High-Res data collection of pre-screened Cryo-EM grids.

Training

Anyone who is interested; especially frequent users; can receive hands-on training and learn how to drive an EPU to perform screening, assisted by Beckman Cryo-EM Center staff. Users interested in training should mention this when signing up for a screening session, as they will need to be approved for access to Singh Center which currently does not allow any visitors. 

As a reminder, the Beckman Center for Cryo-Electron Microscopy is designated as a BSL-1 facility. If your project requires BSL-2, please contact the CEMAC committee for further discussion.


Rates

CURRENT RATES FOR HIGH-RESOLUTION CRYO-ELECTRON MICROSCOPY

  • Internal Independent Use (UPenn and CHOP only): $1,000.00/day + $18.00/C-Clip
  • Academic Independent Use (Temple, Drexel, Jefferson, etc.): $1,630.00/day + $28.50/C-Clip
  • Corporate Independent Use (External): $2,000.00/day + $28.50/C-Clip

iLab Setup

The next step after you fill the consultation form is to set up your iLab account to access services. Learn more about iLab setup.

 

 

ZEISS CROSSBEAM 550

Crossbeam 550 + Meteor

1.Zeiss LSM900 Confocal - 2.Airyscan - 3.Linkam cryostage CMS196 - 4.Zen Connect Blue 

ZEISS LSM900 confocal Airyscan
1.Zeiss LSM900 Confocal - 2.Airyscan - 3.Linkam cryostage CMS196 - 4.Zen Connect Blue 

 

Leica EM GP2

VITROBOT - Thermoscientific

Krios G3i