CyTOF Mass Cytometer

Institute for Immunology Research Resources in partnership with the Philadelphia VAMC

Starting Monday 7/13/2020, CyTOF Service Center will resume Phase II research activities at 50% capacity. All PI’s are asked to provide written justifications for the work to be conducted in CyTOF Service Center during Phase II to the Technical Director, Dr. Takuya Ohtani (takuya@pennmedicine.upenn.edu), and the Scientific Director and the Director of the Institute for Immunology, Dr. E. John Wherry (wherry@pennmedicine.upenn.edu) by email. Please note CyTOF Service Center will limit its use to PENN users during Phase II. 

Mass Cytometry

The CyTOF2 is a new generation flow cytometer, which utilizes Time-Of-Flight mass spectrometer as a readout.  The system uses heavy metal labeled antibodies instead of fluorescent–labeled antibodies, which allows multi-parametric single cell analysis for more than 40 channels with minimal background and compensation problems.

The CyTOF was purchased by the VISN4 HCHT Equipment Funds awarded to the Philadelphia VAMC (PVAMC) for shared use by investigators at both PVAMC and Penn.

IFI offers a wide variety of CyTOF-related services including consultation, training, antibody-conjugation, antibody distribution, and data acquisition. Please contact Takuya Ohtani for more information on services.

User Rates

 As of September 1, 2020, the Institute for Immunology will charge for the following services at the rates listed below:  

Internal Users:

  • Consultation: $65 per hour
  • Data analysis/data preparation: $80 per hour
  • Reagent Distribution : $20 service fee plus the cost of the reagent(s)
  • Sample Acquisition Penn Users: $190 per hour

External Academic Users:

  • Consultation: $85 per hour
  • Data analysis/data preparation: $105 per hour
  • Reagent Distribution : $30 service fee plus the cost of the reagent(s)
  • Sample Acquisition $260 per hour

External Corporate Users:

  • Consultation: $110 per hour
  • Data analysis/data preparation $142 per hour
  • Reagent Distribution : $40 service fee plus the cost of the reagent(s)
  • Sample Acquisition $323 per hour

CyTOF Core Service details

slide 1

Facility Location

A405, Medical Research Building 
Philadelphia VA Medical Center
3900 Woodland Avenue
Philadelphia, PA 19104
215-823-6967


Contact Information

Takuya Ohtani, PhD

Takuya Ohtani, PhD

Technical Director

357 BRB
Tel: 215-573-1006
Fax: 215-573-6804
Email:  takuya@pennmedicine.upenn.edu

John Wherry, PhD

John Wherry, PhD

Scientific Director

354 Biomedical Research Building II/III
Email: wherry@pennmedicine.upenn.edu

Kyong-Mi Chang, MD

Kyong-Mi Chang, MD

Associate Chief of Staff for R&D

Philadelphia VA Medical Center
E-mail: kmchang@pennmedicine.upenn.eduKyong-Mi.Chang@va.gov