Anna Kashina



Room 143 Rosenthal

3800 Spruce Street

Philadelphia, PA 19104

Research Description

Genomes of higher mammals encode an estimated 40,000 proteins, however the complexity of the functions performed by these proteins in vivo is at least an order of magnitude higher. This complexity is achieved in a large part by posttranslational modifications that modulate structure and functions of proteins after synthesis, thus increasing the variety of forms in which the proteins encoded by the same gene can exist in vivo. Evidence suggests that posttranslational modifications constitute a major mechanism for regulation of normal metabolism and disease in higher vertebrates. Discovery and understanding of new posttranslational modifications and uncovering the biological role of the poorly understood modifications constitutes a major emerging field.

The goal of our research is to investigate the physiological role of a previously uncharacterized posttranslational modification, protein arginylation. Knockout of the enzyme responsible for arginylation, ATE1, results embryonic lethality in mice and multiple defects related to heart development and blood vessel remodeling (angiogenesis). Our recent work showed that arginylation regulates many proteins involved in cytoskeleton, cell motility, signaling, and metabolism, and uncovered some mechanisms of this regulation..

Our current studies are focused on three major directions: (1) identification of the ATE1 protein targets and studying the effect of arginylation on their properties and functions; (2) studies of the structure and molecular properties of the mouse ATE1 enzymes; and (3) discovering the mechanisms and pathways that lead to the global physiological effects of protein arginylation.

Degrees & Education

BS (Biochemistry) Moscow State University, 1986

MS (Biochemistry) Moscow State University, 1987

PhD (Cell Biology) Moscow Institute for Protein Research, 1993

Honors & Awards

Early Career Life Scientist Award, American Society for Cell Biology, 2010

Semi-finalist, Howard Hughes Medical Institute Early Career Scientist Competition, 2009

Pfizer Award for Research Excellence, 2007

American Cancer Society, Senior Postdoctoral Fellowship, 1996-1998

Other Perelman School of Medicine Affiliations

Cell and Molecular Biology Graduate Group

University of Pennsylvania School of Veterinary Medicine

Professional Affiliations

Invited Editor, Proceedings of the National Academy of Sciences, USA, 2012

Member, University of Pennsylvania Cardiovascular Institute, 2009-present

Associate Member, University of Pennsylvania Abramson Cancer Center, 2008-present

Member, University of Pennsylvania Institute for Regenerative Medicine, 2008-present

Member, University of Pennsylvania Genomic Frontiers Institute, 2008-present

Ad hoc reviewer, National Science Foundation and US Civilian Research and Development Foundation, 2005-present

Member, Pennsylvania Muscle Institute, 2004-present

Member, Mari Lowe Cancer Center for Comparative Oncology, 2004-present

Peer reviewer for multiple scientific journals (including Nature, Journal of Cell Science, FEBS Letters, Breast Cancer Research and Treatment, Analytical Biochemistry, Chemistry and Biology, Trends in Biochemical Sciences, and others), 1996-present

Member, American Society for Cell Biology, 1994-present

Recent Publications
November 22, 2023
Global Analysis of Post-Translational Side-Chain Arginylation Using Pan-Arginylation Antibodies
Anna Kashina, Ph.D.
MacTaggart B, Shimogawa M, Lougee M, Tang HY, Petersson EJ, Kashina A. - DOI: 10.1016/j.mcpro.2023.100664
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June 28, 2023
The N-degron pathway mediates lipophagy: The chemical modulation of lipophagy in obesity and NAFLD
Anna Kashina, Ph.D.
Jung EJ, Sung KW, Bae TH, Kim HY, Choi HR, Kim SH, Jung CH, Mun SR, Son YS, Kim S, Suh YH, Kashina A, Park JW, Kwon YT. Metabolism. 2023 Jun 28;146:155644. doi:10.1016/j.metabol.2023.155644. PMID: 37385404
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October 21, 2022
Differential N-terminal processing of beta and gamma actin
Anna Kashina, Ph.D.
Chen L, Vedula P, Tang HY, Dong DW, Kashina AS. iScience. 2022 Sep 23;25(10):105186. doi: 10.1016/j.isci.2022.105186. eCollection 2022 Oct 21. PMID: 36248738
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September 21, 2022
N-terminal acetylation and arginylation of actin determines the architecture and assembly rate of linear and branched actin networks
Anna Kashina, Ph.D.
Chin SM, Hatano T, Sivashanmugam L, Suchenko A, Kashina AS, Balasubramanian MK, Jansen S. J Biol Chem. 2022 Sep 21:102518. doi: 10.1016/j.jbc.2022.102518. Online ahead of print. PMID: 36152749
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September 5, 2022
Functional Interplay between Arginyl-tRNA Synthetases and Arginyltransferase
Anna Kashina, Ph.D.
Avcilar-Kucukgoze I, MacTaggart B, Kashina A. Int J Mol Sci. 2022 Sep 5;23(17):10160. doi: 10.3390/ijms231710160. PMID: 36077558
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June 5, 2022
Arginyl-tRNA-protein transferase 1 (ATE1) promotes melanoma cell growth and migration
Anna Kashina, Ph.D.
Lazar I, Fabre B, Feng Y, Khateb A, Frit P, Kashina A, Zhang T, Avitan-Hersh E, Kim H, Brown K, Topisirovic I, Ronai ZA. FEBS Lett. 2022 Jun;596(11):1468-1480. doi: 10.1002/1873-3468.14376. Epub 2022 May 20. PMID: 35561126.
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May 4, 2022
Cysteine-Based Mimic of Arginylation Reproduces Neuroprotective Effects of the Authentic Post-Translational Modification on α-Synuclein
Anna Kashina, Ph.D.
Pan B, Shimogawa M, Zhao J, Rhoades E, Kashina A, Petersson EJ. DOI: 10.1021/jacs.2c02499
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April 8, 2022
α-Synuclein arginylation in the human brain
Anna Kashina, Ph.D.
Zhao J, Pan B, Fina M, Huang Y, Shimogawa M, Luk KC, Rhoades E, Petersson EJ, Dong DW, Kashina A. DOI: 10.1186/s40035-022-00295-0
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January 21, 2022
Arginylation Regulates G-protein Signaling in the Retina
Anna Kashina, Ph.D.
Fina ME, Wang J, Vedula P, Tang HY, Kashina A, Dong DW - doi: 10.3389/fcell.2021.807345
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December 28, 2021
Availability of Arg, but Not tRNA, Is a Rate-Limiting Factor for Intracellular Arginylation
Anna Kashina, Ph.D.
Avcilar-Kucukgoze I, MacTaggart B, Kashina A - . doi: 10.3390/ijms23010314
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July 29, 2021
Post-translational Modifications of the Protein Termini.
Anna Kashina, Ph.D.
Chen L, Kashina A. - doi: 10.3389/fcell.2021.719590.
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December 9, 2020
Purification and Use of tRNA for Enzymatic Post-translational Addition of Amino Acids to Proteins
Anna Kashina, Ph.D.
Avcilar-Kucukgoze I, Gamper H, Hou YM, Kashina A - DOI: 10.1016/j.xpro.2020.100207
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December 17, 2020
Hijacking tRNAs From Translation: Regulatory Functions of tRNAs in Mammalian Cell Physiology
Anna Kashina, Ph.D.
Avcilar-Kucukgoze I, Kashina A - DOI: 10.3389/fmolb.2020.610617
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July 16, 2020
Derived Fragments Can Serve as Arginine Donors for Protein Arginylation
Anna Kashina, Ph.D.
Avcilar-Kucukgoze I, Gamper H, Polte C, Ignatova Z, Kraetzner R, Shtutman M, Hou YM, Dong DW, Kashina A. - DOI: 10.1016/j.chembiol.2020.05.013
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