Jianxin You, Ph.D.

Description of Research Expertise

Research Interests

* Human papillomaviruses-host interaction during persistent latent infection and tumorigenesis

Key words: DNA tumor viruses, Host targets, Persistent latent infection, Episome maintenance, Oncogenesis, Tumor virology, Gene therapy,

Research Details

Cervical carcinomas are associated with high-risk type human papillomaviruses (HPVs) such as HPV-16 and -18. The important cellular events that caused the HPV-infected cells to develop into invasive tumor do not occur until many years after the initial infection, supporting that persistent infection with high-risk HPVs is required for the progression to invasive carcinoma. Research in our laboratory focuses on understanding the virus-host interactions that allow high-risk HPV to establish and maintain persistent infection in host cells.

Papillomaviruses establish persistent latent infection by maintaining the viral genome as autonomous replicating plasmid (episome) in infected cells. To ensure the faithful partitioning of replicated viral episomes to daughter cells during host cell division, the viral E2 protein tethers the viral episomes to host chromosomes during mitosis. Using the bovine papillomavirus (BPV) as a model system, we have identified the cellular bromodomain protein Brd4 as the anchor for E2/viral episome complex on host mitotic chromosomes. However, how the human papillomaviruses are maintained in host cells during latent infection is less clear.

Our major research goals are: 1) to understand the mechanisms that mediate the persistent maintenance of HPVs in host cells, 2) to identify novel cellular targets of this oncogenic DNA tumor virus and to investigate how they regulate papillomavirus life cycle to achieve viral persistence and malignant progression, and 3) to investigate how dysfunction of important cellular apparatus hijacked by viral pathogen could lead to human diseases. Our research integrates biochemistry, proteomics, cell biology and molecular genetics to study how the molecular interplays between oncogenic DNA tumor viruses and their host targets contribute to tumorigenesis. Knowledge on the nature of this virus-host interaction could facilitate the development of novel therapies for treating high-risk HPV latent infection and associated human cancers.


Rotation Projects
# There are several possible projects in the areas of papillomaviruses-host interaction and tumorigenesis. Students are encouraged to contact Dr. You to discuss possible rotation projects.

Lab Personnel

Junpeng Yan, Postdoctoral fellow
Susan Sheng, Research assistant
Sante Mastriana, Research assistant
Jing Jiao, Research specialist
Jason Diaz, Ph.D. Student
Christine M. Helfer, Ph.D. Student