Research in the Davidson Laboratory is focused on inherited genetic diseases that cause central nervous system dysfunction, with a focus on:
i) recessive, childhood onset neurodegenerative disease, such as the lysosomal storage diseases mucopolysaccharidoses and Battens disease;
ii) dominant genetic diseases, specifically the CAG repeat disorders, Huntington’s disease and spinal cerebellar ataxia;
iii) understanding how changes in the transcriptome impact neural development and neurodegenerative disease processes.
Our research on childhood onset neurodegenerative diseases is focused on experiments to better understand the biochemistry and cell biology of proteins deficient in these disorders, and to develop small molecule or gene therapy based strategies for therapy. In recent work, we demonstrated that the application of recombinant viral vectors to various models of storage disease reversed CNS deficits and improved life span. We continue to develop novel vector systems to improve therapeutic outcomes.
Therapies for dominant disorders are an exciting challenge and require that the dominant disease allele be silenced. To approach this, we developed reagents for expressing inhibitory RNAs or editing machinery (e.g., CrispR/Cas9 approaches) in vivo to improve disease phenotypes in relevant animal models.
Finally, we investigate how the transcriptome is altered in neurological diseases. Evaluation of splicing changes has led us to discover novel players in disease pathogenesis that include noncoding RNAs and RNA binding proteins. This work is revealing new pathways of pathogenesis and novel targets for therapy.
Chen YH, Zheng S, Tecedor L, Davidson BL: Overcoming limitations inherent in sulfamidase to improve mucopolysaccharidosis IIIA gene therapy. Mol Ther 26(4): 1118-1126, April 2018 Notes: pii: S1525-0016(18)30017-0; https://doi.org/10.1016/j.ymthe.2018.01.010 (Epub ahead of print].
Amado DA, Rieders JM, Diatta F, Hernandez-Con P, Singer A, Zhang J, Lancaster E, Davidson BL, Chen-Plotkin AS.: AAV-mediated progranulin delivery to a mouse model of progranulin deficiency causes T cell-mediated hippocampal degeneration. bioRxiv. Cold Spring Harbor Laboratory, April 2018 Notes: doi: https://doi.org/10.1101/308692.
Child DD, Lee JH, Pascua CJ, Chen YH, Mas Monteys A, Davidson BL: Cardiac mTORC1 Dysregulation Impacts Stress Adaptation and Survival in Huntington's Disease. Cell Rep 23(4): 1020-1033, April 2018 Notes: doi: 10.1016/j.celrep.2018.03.117.
Schultz ML, Tecedor L, Lysenko E, Ramachandran S, Stein CS, Davidson BL: Modulating membrane fluidity corrects Batten disease phenotypes in vitro and in vivo. Neurobiol Dis 115: 182-193, April 2018 Notes: doi: 10.1016/j.nbd.2018.04.010. [Epub ahead of print]
Victor MB, Richner M, Olsen HE, Lee SW, Monteys AM, Ma C, Huh CJ, Zhang B, Davidson BL, Yang XW, Yoo AS: Striatal neurons directly converted from Huntington's disease patient fibroblasts recapitulate age-associated disease phenotypes. Nature Neuroscience 21(3): 341-352, March 2018 Notes: doi: 10.1038/s41593-018-0075-7 [Epub ahead of print]
Machuca TN, Cypel M, Bonato R, Yeung J, Chun Y-M, Juvet S, Guan Z, Hwang DM, Chen M, Saito T, Harmantas C, Davidson BL, Waddell TK, Liu M, Keshavjee S. : Safety and efficacy of ex-vivo donor lung adenoviral IL-10 gene therapy in a large animal lung transplant survival model. Hum Gen Ther 28(9): 757-765, Sept 2017.
Dissen GA, Adachi K, Lomniczi A, Chatkupt T, Davidson BL, Nakai H, Ojeda SR: Engineering as a gene silencing viral construct that targets the cat hypothalamus to induce permanent sterility: An update. Reprod Dom Anim 52(Suppl 2): 354-358, April 2017 Notes: doi: 10.111/rda. 12834 [Epub ahead of print]
Carmona V, Cunha-Santos J, Onofre I, Simoes A, Vijayakumar U, Davidson BL, Pereira de Almeida L.: Unravelling endogenous MicroRNAs system dysfunction as a new pathophysiological mechanism in Machado-Joseph disease. Mol Ther 25(4): 1038-1055, Feb 2017.
Kim Y-C, Miller A, Lins LCRF, Han W-W, Keiser MS, Davidson BL, Nandakumar S.: RNA interference of human alpha-synuclein in mouse. Front Neurol 8, Jan 2017 Notes: 10.3389/fneur2017.00013. Volume 8, Article 13.
Monteys, AM, Ebanks SA, Keiser MS, Davidson BL: CRISPR/Cas9 editing of the mutant Huntingtin allele in vitro and in vivo. Mol Ther 25(1): 12-23, Jan 2017.
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Last updated: 07/11/2018
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