Penn Cardiovascular Institute

Penn Cardiovascular Institute Research Directory

faculty photo

Daniel J. Rader, M.D.

Seymour Gray Professor of Molecular Medicine
Department: Medicine

Contact information
Perelman School of Medicine
University of Pennsylvania
11-125 Smilow Center for Translational Research
3400 Civic Center Blvd
Philadelphia, PA 19104-5158
Office: (215) 573-4176
Fax: (215) 573-8606
Education:
B.A.
Lehigh University, 1981.
M.D.
Medical College of Pennsylvania, 1984.
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Description of CVI Expertise

Etiology of HDL Cholesterol ("The Upenn High HDL Research Study")

Penn CVI Scientific Director for Translational / Clinical Research

CVI Program Unit Administrator (Director):
Prevention / Atherosclerosis / Lipids

Research Interests
The Rader laboratory is focused on two major themes: 1) novel pathways regulating lipid and lipoprotein metabolism and atherosclerosis inspired by unbiased studies of human genetics; 2) factors regulating the structure and function of high density lipoproteins and the process of reverse cholesterol transport and their relationship to atherosclerosis. A variety of basic cell and molecular laboratory techniques, mouse models, and translational research approaches are used in addressing these questions.

Some examples of ongoing projects are:
1) The roles of sortilin (gene SORT1) and tribbles-1 (gene TRIB1) in lipoprotein metabolism and atherosclerosis. Variants at the SORT1 locus are among the most strongly associated with LDL cholesterol and (coronary artery disease) in the human genome, and variants at the TRIB1 locus are significantly associated with all major plasma lipid traits and CAD. A variety of tissue-specific deleted mouse models, gene targeting in iPS cells with differentiation to hepatocytes, and cell biologic and biochemical approaches are being employed.

2) Functional genomics and mechanistic studies of a number of additional genes at loci significantly associated with lipid and metabolic traits, CAD, or other cardiovascular traits. Most of these genes harbor rare coding variants associated with these traits. In addition to elucidating fundamental mechanisms by which the protein influences relevant biology, the influence of specific mutations on protein structure and function are being explored.

3) Molecular regulation of HDLmetabolism and reverse cholesterol transport using cells, mice, and humans

4) Deep phenotyping of humans with low-frequency and rare variants in genes influencing lipid and cardiovascular traits, including the generation of iPS cells and differentiation to a variety of relevant cell types

Administrative Assistant:
Linda Carmichael, 215-573-4176

Executive Assistant:
Cathy Warford, 215-573-7272

Grants Manager:
Ted Panczyszyn, 215-573-1264


Research Lab:
11th floor, Smilow Center for Translational Research

Clinical Research:
8th floor Maloney Building, Hospital of The University of Pennsylvania


Lab Personnel:

Research Assistant Professors:
Marina Cuchel, MD/PhD
Yanqing (Anna) Gong, PhD
Nicholas Hand, PhD

Adjunct Professors:
Sissel Lund-Katz, PhD
Michael C. Phillips, PhD

Senior Research Investigators:
Jeffrey Bilheimer, PhD
John Millar, PhD

Research Associates:
Salam Ibrahim, PhD
Nicholas Lyssenko, PhD
Sony Tuteja, PharmD

Post-doctoral Fellows:
Robert Bauer, PhD
Xin Bi, PhD
Donna Conlon, PhD
Marie Guerraty, MD/PhD
Ali Javaheri, MD/PhD
Sylvia Nürnberg, PhD
Evanthia Pashos, PhD
Junichiro Tohyama, MD/PhD

Visiting Scientists:
Jian Cui, MD
Hongyu (Angela) Han, PhD
Adriaan (Onno) Holleboom, PhD
Batuhan Yenilmez
Bijun Zhao

Graduate Students:
Devin Christopher
Sumeet Khetarpal
HyeIn (Lucy) Kim
Wen Lin
Minal Mehta
Kevin Patel
Cecilia Vitali
Christopher Yu
Lingdi Zhang

Project Managers:
Stephanie DerOhannessian, MB
Dawn Marchadier, MS
Megan Mucksavage, MTR

Biostatistician:
Hanumantha (Shanker) Rao, MS
Liming Qu, MS
Wei Zhao, MS

Research Specialists:
Debbie Cromley
Edwige Edouard
Susannah Elwyn, MS
Stacey Lytle
Mayda Hernandez, MS
Phyllis May
Mary McCoy, PhD
Linda Morrell
Antonino Picataggi
Amrith Rodrigues, MS
Mikhaila Smith
Mao-Sen Sun, MD/PhD
Kevin Trindade
Aisha Wilson, MLAS


Clinical Research Personnel:

Project Managers:
Amanda Baer, MBA

Data Coordinators:
Marjorie Risman, MS

Clinical Research Coordinators:
Canita Brent
Maria Escobar
James McParland
Sharon Molino
Tracey Sikora
Masako Ueda, MD





The Etiology of HDL Cholesterol (“The Upenn High HDL Research Study”)



Name of Study: The Etiology of HDL Cholesterol (“The Upenn High HDL Research Study”)

Principal Investigator: Daniel J. Rader, MD

Subjects Enrolled: 2690 subjects in 1196 families as of January 28, 2013


What is HDL? The letters HDL stand for high density lipoprotein. It is known as the “good cholesterol” because a high HDL level can protect against heart disease and stroke.

Overview: The purpose of this study is to identify genes that are associated with HDL and influence coronary artery disease. Data from blood samples and health histories of individuals with high HDL levels and their family members are used to assess the relationship between these genes and HDL cholesterol. The long term goal of investigating these genes is the development of new therapies to prevent coronary artery disease.

A note to people who have already participated and their families: If you have already provided a blood sample for this study since 1998, thank you very much! Your help is invaluable. If you have high HDL cholesterol, we are asking that you speak to your relatives who have not yet participated. We are interested in receiving samples from your parents, children and siblings. If a participant’s child also participated, or plans to participate, we would also like to receive a sample from the child’s other parent.

If it is possible to receive samples that would give us samples from two parents and at least one child, whether you are the child or one of the parents, your family’s participation would be especially valuable and welcome.

A note to potential new participants: If you and your family have NOT participated in the study, but you have been told you have high HDL (for example you are a man with an HDL value above 75 mg/dL or a woman with an HDL above 90 mg/dL) you may also be eligible to participate.

Contact Information:

For more information about the study, or to arrange participation, contact the research coordinator:

Marjorie Risman
risman@mail.med.upenn.edu
local telephone number: 215-746-8342
toll free telephone number: 1-888-81HEART (1-888-814-3278).

What does participating in this study involve?


Participants are asked to provide a small blood sample and complete a short health questionnaire. We use the blood sample to measure cholesterol levels and to isolate DNA for the genetic research. DNA is the part of your blood sample that holds genetic information. Participants receive the results of their lipid panel (cholesterol results).

A participant can either:

Come to our research clinic at the Hospital of the University of Pennsylvania for a brief visit to provide a blood sample.

OR

Receive by mail a kit containing all the necessary materials for the blood draw. The kit can be taken to a local laboratory or doctor’s office and the blood sample can then be shipped to us at our expense in packaging we provide. If a participant is charged for having blood drawn, we can reimburse the charge. We can also assist in finding a location for the blood draw.


Research Results



Research Results: The study has been recruiting participants since 1998, and the investigators have already made some significant findings using the data already collected.

A specific protein, endothelial lipase, breaks down HDL cholesterol particles. The following three articles used data from this study to explore the relationship between endothelial lipase and HDL:


1. deLemos AS, Wolfe ML, Long CJ, Sivapackianathan R, Rader DJ. (2002) Identification of genetic variants in endothelial lipase in persons with elevated high-density lipoprotein cholesterol. Circulation. 2002;106(11):1321-6.

Shortly after the initial discovery of the endothelial lipase gene, we sequenced the gene in subjects with high HDL levels. We identified six variants of endothelial lipase that may have contributed to the individuals' elevated HDL concentration.

» http://circ.ahajournals.org/cgi/content/full/106/11/1321%20%20

» http://circ.ahajournals.org/cgi/reprint/106/11/1321


2. Edmondson AC, Brown RJ, Kathiresan S, Cupples LA, Demissie S, Manning AK, Jensen MK, Rimm EB, Wang J, Rodrigues A, Bamba V, Khetarpal SA, Wolfe ML, Derohannessian S, Li M, Reilly MP, Aberle J, Evans D, Hegele RA, Rader DJ. (2009) Loss-of-function variants in endothelial lipase are a cause of elevated HDL cholesterol in humans. J. Clin. Invest. 119: 1042-1050.

» http://www.jci.org/articles/view/37176

» http://www.jci.org/articles/view/37176/pdf

We identified that healthy people with high HDL also have mutations in their endothelial lipase protein that makes the protein unable to break down HDL cholesterol particles. Our results suggest that new drugs that stop endothelial lipase from breaking down HDL cholesterol particles will increase HDL levels and may reduce heart disease. However, further studies on the effect of the endothelial lipase mutations in heart disease are needed.


3. Brown RJ, Edmondson AC, Griffon N, Hill TB, Fuki IV, Badellino KO, Li M, Wolfe ML, Reilly MP, Rader DJ (2009) A naturally occurring variant of endothelial lipase associated with elevated HDL exhibits impaired synthesis. J Lipid Res.

» http://www.jlr.org/content/50/9/1910.long

» http://www.jlr.org/content/50/9/1910.full.pdf+html

We identified a version of the endothelial lipase that is present in some individuals of African descent that results in a person having lower levels of endothelial lipase protein and higher levels of HDL.


4. He J, Wang K, Edmondson AC, Rader DJ, Li C, Li M. (2010) Gene-based interaction analysis by incorporating external linkage disequilibrium information. Eur J Hum Genet. 1-9

» http://www.nature.com/ejhg/journal/vaop/ncurrent/full/ejhg2010164a.html

» http://www.nature.com/ejhg/journal/vaop/ncurrent/pdf/ejhg2010164a.pdf  

We developed a new statistical method to identify genes that interact with each other.  The Upenn High HDL Research Study was used to show how this new method works and identified the genes CETP and BCAT1 interacting with each other.


5. Teslovich TM, Musunuru K, Smith AV, Edmondson AC, Stylianou IM, Koseki M, Pirruccello JP, Ripatti S, Chasman DI, Willer CJ, Johansen CT, Fouchier SW, Isaacs A, Peloso GM, Barbalic M, Ricketts SL, Bis JC, Aulchenko YS, Thorleifsson G, Feitosa MF, Chambers J, Orho-Melander M, Melander O, Johnson T, Li X, Guo X, Li M, Shin Cho Y, Jin Go M, Jin Kim Y, Lee JY, Park T, Kim K, Sim X, Twee-Hee Ong R, Croteau-Chonka DC, Lange LA, Smith JD, Song K, Hua Zhao J, Yuan X, Luan J, Lamina C, Ziegler A, Zhang W, Zee RY, Wright AF, Witteman JC, Wilson JF, Willemsen G, Wichmann HE, Whitfield JB, Waterworth DM, Wareham NJ, Waeber G, Vollenweider P, Voight BF, Vitart V, Uitterlinden AG, Uda M, Tuomilehto J, Thompson JR, Tanaka T, Surakka I, Stringham HM, Spector TD, Soranzo N, Smit JH, Sinisalo J, Silander K, Sijbrands EJ, Scuteri A, Scott J, Schlessinger D, Sanna S, Salomaa V, Saharinen J, Sabatti C, Ruokonen A, Rudan I, Rose LM, Roberts R, Rieder M, Psaty BM, Pramstaller PP, Pichler I, Perola M, Penninx BW, Pedersen NL, Pattaro C, Parker AN, Pare G, Oostra BA, O'Donnell CJ, Nieminen MS, Nickerson DA, Montgomery GW, Meitinger T, McPherson R, McCarthy MI, McArdle W, Masson D, Martin NG, Marroni F, Mangino M, Magnusson PK, Lucas G, Luben R, Loos RJ, Lokki ML, Lettre G, Langenberg C, Launer LJ, Lakatta EG, Laaksonen R, Kyvik KO, Kronenberg F, König IR, Khaw KT, Kaprio J, Kaplan LM, Johansson A, Jarvelin MR, Cecile J W Janssens A, Ingelsson E, Igl W, Kees Hovingh G, Hottenga JJ, Hofman A, Hicks AA, Hengstenberg C, Heid IM, Hayward C, Havulinna AS, Hastie ND, Harris TB, Haritunians T, Hall AS, Gyllensten U, Guiducci C, Groop LC, Gonzalez E, Gieger C, Freimer NB, Ferrucci L, Erdmann J, Elliott P, Ejebe KG, Döring A, Dominiczak AF, Demissie S, Deloukas P, de Geus EJ, de Faire U, Crawford G, Collins FS, Chen YD, Caulfield MJ, Campbell H, Burtt NP, Bonnycastle LL, Boomsma DI, Boekholdt SM, Bergman RN, Barroso I, Bandinelli S, Ballantyne CM, Assimes TL, Quertermous T, Altshuler D, Seielstad M, Wong TY, Tai ES, Feranil AB, Kuzawa CW, Adair LS, Taylor HA Jr, Borecki IB, Gabriel SB, Wilson JG, Holm H, Thorsteinsdottir U, Gudnason V, Krauss RM, Mohlke KL, Ordovas JM, Munroe PB, Kooner JS, Tall AR, Hegele RA, Kastelein JJ, Schadt EE, Rotter JI, Boerwinkle E, Strachan DP, Mooser V, Stefansson K, Reilly MP, Samani NJ, Schunkert H, Cupples LA, Sandhu MS, Ridker PM, Rader DJ, van Duijn CM, Peltonen L, Abecasis GR, Boehnke M, Kathiresan S. Biological, clinical and population relevance of 95 loci for blood lipids. Nature 466:707-713.

» http://www.nature.com/nature/journal/v466/n7307/full/nature09270.html

» http://www.nature.com/nature/journal/v466/n7307/pdf/nature09270.pdf

We collaborated with many different studies to study the genetics of plasma lipids in >100,000 individuals.  We identified 95 different regions of the human genome that contribute to determining levels of total cholesterol, LDL cholesterol, HDL cholesterol, and triglycerides and showed genetic contributions to cholesterol levels in people of European, East Asian, South Asian, and African American ancestry.  This knowledge will help us to better understand the biology of cholesterol metabolism and identify new drug targets to prevent heart disease.

Contact Us

Marjorie Risman

risman@mail.med.upenn.edu
Local telephone number: 215-746-8342
Toll free telephone number: 1-888-81HEART (1-888-814-3278)




Selected Publications

Millar JS, Reyes-Soffer G, Jumes P, Dunbar RL, deGoma EM, Baer AL, Karmally W, Donovan DS, Rafeek H, Pollan L, Tohyama J, Johnson-Levonas AO, Wagner JA, Holleran S, Obunike J, Liu Y, Ramakrishnan R, Lassman ME, Gutstein DE, Ginsberg HN, Rader DJ. : Anacetrapib lowers LDL by increasing ApoB clearance in mildly hypercholesterolemic subjects. J Clin Invest May 2015 Notes: [Epub ahead of print]

deGoma EM, Salavati A, Shinohara RT, Saboury B, Pollan L, Schoen M, Torigian DA, Mohler ER, Dunbar RL, Litt HI, Woo J, Rader DJ, Alavi A, Mehta NN.: A Pilot Trial to Examine the Effect of High-Dose Niacin on Arterial Wall Inflammation Using Fluorodeoxyglucose Positron Emission Tomography. Acad Radiol 22(5): 600-609, May 2015.

Rader DJ: Human genetics of atherothrombotic disease and its risk factors. Arterioscler Thromb Vasc Biol 35(4): 741-747, Apr 2015.

Zhang H, Xue C, Shah R, Bermingham K, Hinkle CC, Li W, Rodrigues A, Tabita-Martinez J, Millar JS, Cuchel M, Pashos E, Liu Y, Yan R, Yang W, Gosai SJ, VanDorn D, Chou S, Gregory BD, Morrisey E, Li M, Rader DJ, Reilly MP: Functional Analysis and Transcriptomic Profiling of iPSC-Derived Macrophages and Their Application in Modeling Mendelian Disease. Circ Res Apr 2015 Notes: [Epub ahead of print]

Agarwala AP, Rodrigues A, Risman M, McCoy M, Trindade K, Qu L, Cuchel M, Billheimer J, Rader DJ. : High-Density Lipoprotein (HDL) Phospholipid Content and Cholesterol Efflux Capacity Are Reduced in Patients With Very High HDL Cholesterol and Coronary Disease. Arterioscler Thromb Vasc Biol Apr 2015 Notes: Epub ahead of print

Stitziel NO, Peloso GM, Abifadel M, Cefalu AB, Fouchier S, Motazacker MM, Tada H, Larach DB, Awan Z, Haller JF, Pullinger CR, Varret M, Rabès JP, Noto D, Tarugi P, Kawashiri MA, Nohara A, Yamagishi M, Risman M, Deo R, Ruel I, Shendure J, Nickerson DA, Wilson JG, Rich SS, Gupta N, Farlow DN, Neale BM, Daly MJ, Kane JP, Freeman MW, Genest J, Rader DJ, Mabuchi H, Kastelein JJ, Hovingh GK, Averna MR, Gabriel S, Boileau C, Kathiresan S: Exome sequencing in suspected monogenic dyslipidemias. Circ Cardiovasc Genet 8(2): 343-350, Apr 2015.

Nelson CP, Hamby SE, Saleheen D, Hopewell JC, Zeng L, Assimes TL, Kanoni S, Willenborg C, Burgess S, Amouyel P, Anand S, Blankenberg S, Boehm BO, Clarke RJ, Collins R, Dedoussis G, Farrall M, Franks PW, Groop L, Hall AS, Hamsten A, Hengstenberg C, Hovingh GK, Ingelsson E, Kathiresan S, Kee F, König IR, Kooner J, Lehtimäki T, März W, McPherson R, Metspalu A, Nieminen MS, O'Donnell CJ, Palmer CN, Peters A, Perola M, Reilly MP, Ripatti S, Roberts R, Salomaa V, Shah SH, Schreiber S, Siegbahn A, Thorsteinsdottir U, Veronesi G, Wareham N, Willer CJ, Zalloua PA, Erdmann J, Deloukas P, Watkins H, Schunkert H, Danesh J, Thompson JR, Samani NJ; CARDIoGRAM+C4D Consortium. : Genetically determined height and coronary artery disease. N Engl J Med. 372(17): 1608-1618, Apr 2015.

Kaess BM, Preis SR, Lieb W, Beiser AS, Yang Q, Chen TC, Hengstenberg C, Erdmann J, Schunkert H, Seshadri S, Vasan RS; CARDIoGRAM, Assimes TL, Deloukas P, Holm H, Kathiresan S, König IR, McPherson R, Reilly MP, Roberts R, Samani NJ, Stewart AF: Circulating brain-derived neurotrophic factor concentrations and the risk of cardiovascular disease in the community. J Am Heart Assoc 4(3): e001544, Mar 2015.

Bauer RC, Tohyama J, Cui J, Cheng L, Yang J, Zhang X, Ou K, Paschos GK, Zheng XL, Parmacek MS, Rader DJ, Reilly MP: Knockout of adamts7, a novel coronary artery disease locus in humans, reduces atherosclerosis in mice. Circulation 131(13): 1202-1213, Mar 2015.

Shah R, Matthews GJ, Shah RY, McLaughlin C, Chen J, Wolman M, Master SR, Chai B, Xie D, Rader DJ, Raj DS, Mehta NN, Budoff M, Fischer MJ, Go AS, Townsend RR, He J, Kusek JW, Feldman HI, Foulkes AS, Reilly MP; CRIC Study Investigators: Serum Fractalkine (CX3CL1) and Cardiovascular Outcomes and Diabetes: Findings From the Chronic Renal Insufficiency Cohort (CRIC) Study. Am J Kidney Dis Mar 2015 Notes: [Epub ahead of print]

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Last updated: 05/12/2015
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