David F. Wilson, PhD
Professor of Biochemistry and Biophysics
Department: Biochemistry and Biophysics
Graduate Group Affiliations
Contact information
901A Stellar-Chance Laboratories
Philadelphia, PA 19104-6059
Philadelphia, PA 19104-6059
Office: (215) 898-6382
Fax: (215) 573-3787
Fax: (215) 573-3787
Email:
wilsondf@mail.med.upenn.edu
wilsondf@mail.med.upenn.edu
Publications
Education:
B.S.
Colorado State University, Fort Collins, Colorado , 1959.
Ph.D.
Oregon State University, 1964.
Permanent linkB.S.
Colorado State University, Fort Collins, Colorado , 1959.
Ph.D.
Oregon State University, 1964.
Description of CVI Expertise
CVI Program Unit(s):Cardiovascular Development / Congenital Heart Disease
Biotechnology / Nanomedicine / Imaging
CVI Research Description:
Our research interest is in oxygen delivery to tissue and the role of oxygen in tissue physiology, biochemistry and pathology. Most of our work in recent years has been on hypoxic/ischemic injury to the newborn brain and the role of oxygen in tumor physiology and therapy, but we have long been also interested in the heart.
In pursuing our interests in the role of oxygen in tissue, we have developed an optical method for quantitative measurement, including imaging, in living tissue and in real time. This method uses specially constructed phosphorescent nanosensors to detect and measure oxygen. We are continuing development of this technology both for use in basic research and for possible translation into the clinics.
Selected Publications
Soloviev V.D., Wilson D.F., Vinogradov S.A. : “3D phosphorescence lifetime imaging in turbid media: the forward problem” Applied Optics 42: 113-123, 2003.Wilson, D.F. and Vinogradov, S.A. : “Tissue measurements using phosphorescence quenching.” In: “Fluorescence in Biomedicine” In press. B. Pogue and M-A. Mycek (eds.). Marcel Dekker, Inc. New York, 2003.
Zaitseva, T., Shen, J., Schears, G., Creed, J., Wilson, and Pastuszko, A. : “Effect of catecholamines on activity of Na,K-ATPase in neonatal piglet brain during posthypoxic reoxygenation” Comp. Biochem. and Physiol. 132(1): 139-145, 2002.
Vinogradov, S.A., Kim, E., and Wilson, D.F. : “Pd tetrabenzoporphyrin-dendrimers – near infrared phosphors for oxygen measurements by phosphorescence quenching” In: Biomedical Nanotechnology Architectures and Applications. Proceedings of SPIE. D.J. Bornhop, D.A. Dunn, R.P. Mariella, Jr., et al (eds.). 4626: 193-200, 2002.
Wilson, D.F., Grosul, P., Rozhkov, V., Dugan, B.W., Reitveld, I., and Vinogradov, S.A. : “Oxygen Distributions within Tissue by Phosphorescence Quenching” In: Biomedical Nanotechnology Architectures and Applications. Proceedings of SPIE. D.J. Bornhop, D.A. Dunn, R.P. Mariella, Jr., et al (eds.). 4626: 184-192, 2002.
Vinogradov, S.A., Grosul, P., Rozhkov, V., Dunphy, I., Shuman, L., Dugan, B.W., Evans, S., and Wilson, D.F.: “Oxygen distributions in tissue measured by phosphorescence quenching” Adv. Exptl. Med. Biol. 510: 181-186, 2002.
Rozhkov, V., Wilson, David F., and Vinogradov, S. : “Phosphorescent Pd porphyrin-dendrimers: tuning core accessibility by varying the hydrophobicity of the dendrimer matrix” Macromolecules 35: 1991-1993, 2002.
Zaitseva, T., Creed, J., Schears, G., Shen, J., Knight, K., Wilson, D.F. and Pastuszko, A. : “Effect of hypoxia and ischemia on expression of selected genes in brain of newborn piglets” Adv. Exptl. Med. Biol. 510: 319-324, 2002.
Zaitseva, T., Schears, G., Shen, J., Creed, J., Wilson, D.F., Greeley W.J. and Pastuszko, A. : “Altered gene expression following cardiopulmonary bypass and cardiac arrest” Oxygen Transport to Tissue XXII, Adv. Exptl. Med. Biol., in press 2002.
Schears, G., Shen, J., Creed, J., Zaitseva, T., Wilson, D.F., Greeley, W.J., and Pastuszko, A. : “Brain oxygenation during cardiopulmonary bypass and circulatory arrest” Adv. Exptl. Med. Biol. 510: 325-330, 2002.