TB/HIV Coinfection Home
/ Working Groups
Case Western Reserve University CFAR
- Ongoing HIV/TB Co-infection Projects:
- Impact of TB on HIV-1 infection. Investigation of the impact of TB on immunologic and virologic factors in HIV-1 disease . Zahra Toossi firstname.lastname@example.org
- Mechanisms of increased susceptibility to TB in HIV-1 infected subjects Mechanisms ofincreased susceptibility of mononuclear cells to HIV infection and defects in MHC-II antigen presentation of HIV-1 in dual infection. David Canaday email@example.com
Available Inter-CFAR Shared Resources:
- Dr. R. Silve
Responses of human alveolar macrophages and monocytes to virulent MTB infection
- Dr. D. Canaday
Antigen presentation of virulent MTB by mononuclear phagocytes and dendritic cells
- Dr. L. Nguyen
MTB virulence genes
- Dr. H. Boom
Heterogeneity of T-cells in MTB infection
- Dr. Z. Toossi
Role of TGF-b in Pathogenesis of TB
- Dr. R. Rojas
Role of MTB lipid and protein components in activation of human innate immunity
- Dr. L. Ramachandran
Phagosome-lysosome biology in MTB infection
- Dr. C. Hirsch
Role of regulatory T-cells in immune responses to MTB
- Dr. C. Harding
M. tuberculosis lipoprotein-TLR2 interactions
- Dr. C. Stein
Genetic susceptibility to TB, and the interaction between HIV infection and human risk genotype
- BSL-3 facilities:
Core E (Microbial Pathogenesis) of the CWRU CFAR has promoted research in the study of TB and HIV/TB interaction for over 10 years, and has therefore State-of-the-Art expertise in handling pathogenic MTB safely. This expertise includes the capacity to propagate and assess virulent MTB growth and to assess MTB gene expression. Presently this expertise is in use by CFAR investigators at CWRU, however, our large capacity services can be also made available to interested researchers at other sites.
- Capacities include:
Virulent MTB propagation (Roller Incubator), and quantitation.
Real-time PCR assays for MTB genes including (85A and B, AcR, ribosomal 16S) by Taqman (StepOne). Methodologies for preparation of RNA from MTB or MTB-infected cell samples.
Methodologies and equipment for electroporation of MTB (Biorad-Gene Pulser II).
Methodologies for viable sorting of MTB-infected or exposed mononuclear cells (Elite, Beckman-Coulter).