TB/HIV Coinfection Working Group
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Case Western Reserve University CFAR

Primary contacts

David Canaday
Zahra Toossi

  1. Ongoing HIV/TB Co-infection Projects:
    • Impact of TB on HIV-1 infection.  Investigation of the impact of TB on immunologic and virologic factors in HIV-1 disease .  Zahra Toossi zxt2@case.edu
    • Mechanisms of increased susceptibility to TB in HIV-1 infected subjects  Mechanisms ofincreased susceptibility of mononuclear cells to HIV infection and defects in MHC-II antigen presentation of HIV-1 in dual infection.  David Canaday dxc44@case.edu  
  2. HIV/TB Researchers:
    • Dr. R.  Silve
      Responses of human alveolar macrophages and monocytes to virulent MTB infection
    • Dr. D. Canaday
      Antigen presentation of virulent MTB by mononuclear phagocytes and dendritic cells
    • Dr. L. Nguyen
      MTB virulence genes liem.
    • Dr. H. Boom
      Heterogeneity of T-cells in MTB infection
    • Dr. Z. Toossi
      Role of TGF-b in Pathogenesis of TB
    • Dr. R.  Rojas
      Role of MTB lipid and protein components in activation of human innate immunity
    • Dr. L. Ramachandran
      Phagosome-lysosome biology in MTB infection
    • Dr. C. Hirsch
      Role of regulatory T-cells in immune responses to MTB
    • Dr. C. Harding 
      M. tuberculosis lipoprotein-TLR2 interactions
    • Dr. C. Stein
      Genetic susceptibility to TB, and the interaction between HIV infection and human risk genotype
  3. Available Inter-CFAR Shared Resources:
    • BSL-3 facilities:
      Core E (Microbial Pathogenesis) of the CWRU CFAR has promoted research in the study of TB and HIV/TB interaction for over 10 years, and has therefore State-of-the-Art expertise in handling pathogenic MTB safely.  This expertise includes the capacity to propagate and assess virulent MTB growth and to assess MTB gene expression.  Presently this expertise is in use by CFAR investigators at CWRU, however, our large capacity services can be also made available to interested researchers at other sites.
    • Capacities include:
      Virulent MTB propagation (Roller Incubator), and quantitation. Real-time PCR assays for MTB genes including (85A and B, AcR, ribosomal 16S) by Taqman (StepOne).  Methodologies for preparation of RNA from MTB or MTB-infected cell samples. Methodologies and equipment for electroporation of MTB (Biorad-Gene Pulser II). Methodologies for viable sorting of MTB-infected or exposed mononuclear cells (Elite, Beckman-Coulter).