Metabolic Tracer Resource (Formerly GC/MS Resource)

The Metabolic Tracer Resource provides services to IDOM investigators using stable isotope labeled tracers (typically carbon-13 or deuterium) in cell-based, animal and human metabolic studies.

The Resource offers analysis of stable isotope enrichment of glucose, glycerol, fatty acids and amino acids in samples from metabolic tracer studies. These data can then be used to calculate rates of turnover, synthesis, production or recycling.

The Resource is currently located in room 771, Clinical Research Building (CRB).  Analyses are conducted on an Agilent 7890A gas chromatograph / 5975 mass spectrometer (GC/MS). 

5975 Mass Spectrometer (GC/MS)

Please contact Dr. John Millar for further information.

Lab contact: Vishala Patel
Room 771 CRB
Lab Phone: 215-746-4587
Email: patv@mail.med.upenn.edu

Director: John Millar, PhD
Office: 652 BRB II/III
Office phone: 215-898-0638
Email: jsmillar@mail.med.upenn.edu

© Trustees of the University of Pennsylvania

Metabolic assays:

De novo lipognesis/Fatty acid turnover

Free-living assessment of hepatic glucose production

Stable isotope glucose tolerance test

Glucose recycling

In vivo lipolysis (glycerol production)

Amino acid synthesis and turnover

Protein synthesis and turnover

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