Figure 1. Apoptotic neurodegeneration of dentate gyrus granule neurons and necrotic neurodegeneration of CA1 sector pyramidal neurons in the rostral hippocampus after transient forebrain ischemia. Representative photomicrographs demonstrating Fluoro-Jade (green) and nuclear Hoechst (blue) co-labeling of degenerating neurons in the rostral hippocampus (Bregma -2.2 cm) 72 hours after 10 minutes of transient forebrain ischemia. The low magnification image at the top of the figure demonstrates the overall distribution of post-ischemic neurodegeneration (green Fluoro-Jade labeled cells) in this model. Transparent boxes represent regions examined by high magnification confocal microscopy, which is shown in the lower half of the figure. High magnification confocal images of the lateral dentate gyrus (DG) demonstrate Fluoro-Jade labeled cells with clear apoptotic nuclear morphology (yellow arrows). Neurons with normal nuclei in the same region do not co-label with Fluoro-Jade (gray arrowheads). High magnification confocal images of the CA1 sector pyramidal neurons (CA1) demonstrate Fluoro-Jade labeled neurons that do not have apoptotic nuclear morphology (red arrows). In fact, the nucleus appears to be disintegrated which is consistent with necrotic neurodegeneration.