Figure 4. AAV2/2-mediated EGFP expression in the somas and axons of retinal ganglion cells. Intravitreal injections of AAV2/2-CMV-EGFP were performed 5-6 weeks prior to perfusion fixation. Representative fluorescence microscopy images of optic nerve axons in retinal flat mount and 20 µm thick longitudinal cryosections of optic nerves. Images captured at 100x (left and top right) and 200x (bottom right) magnification.