Professor Walter Englander
Department of Biochemistry & Biophysics
University of Pensylvania
Philadelphia, PA 19104-6059

We are using the MS-HX methodology to determine the folding pathway and cooperative substructure of p38alpha kinase. In collaboration with Professors Cecilia Tommos and Wolfgang Peti.

Professor Bertrand Garcia-Moreno
Department of Biophysics
Johns Hopkins University
A project with postdoctoral associate Dr. Nathaniel Nucci and undergraduate Nimu Sidhu. We are employing reverse micelle encapsulation to explore several anticipated effects of confinement, including protein hydration and dynamics.
Robert Griffin

Professor Robert G. Griffin
Department of Chemistry
Massachusetts Institute of Technology
Cambridge, MA

With Dr. Kathy Valentine, we are taking advantage of the favorable properties of solutions of encapsulated proteins dissolved in low viscosity fluids to enable dynamic nuclear polarization of proteins and nucleic acids in solution.

Songi Han

Professor Songi Han
Department of Chemistry
University of California, Santa Barbara
Santa Barbara, CA

With Dr. Nathaniel Nucci, we are comparing the view of protein hydration provided by our reverse micelle encapsulation strategy with that offered by the nitroxide spin radical Overhauser nuclear polarization method pioneered by Professor Han.

Vince Hilser

Professor Vince Hilser
Department of Biology
Johns Hopkins University
Baltimore, MD

With Drs. Sabrina Bedard and Nathaniel Nucci, we are exploring the use of the confined space of the reverse micelle to stabilize the glucocorticoid receptor for structural characterization by NMR.

Susan Marqusee

Professor Susan Marqusee
Department of Molecular Cell Biology
University of California, Berkeley
Berkeley, CA

With Dr. Jack Lim, we are exploring the evolutionary origins of protein internal motion in ancestrally reconstructed ribonuclease H proteins.

Wolfgang Peti

Professor Wolfgang Peti
Department of Chemistry
Brown University
Providence, RI

With graduate student Kyle Harpole, we are engaged in a multi-PI NIH grant with Professor Peti to explore the role of conformational entropy in small and large ligand interactions with p38α kinase, a major regulatory protein and a high profile target for pharmaceutical intervention.

Professor Kim Sharp
Department of Biochemistry & Biophysics
University of Pensylvania
Philadelphia, PA 19104-6059
With graduate student Vignesh Kasinath, we are using molecular dynamics simulations of various systems to gain insight into the microscopic origins of our “entropy meter” based on NMR relaxation methods.