Upstairs

We maintain a significant effort in molecular biology and preparative biochemistry. In most cases, all of the materials that we use in our biophysical studies are made in-house. As biophysical quantities of materials are required (i.e. tens of mgs), often isotopically enriched, we have a full array of supporting technology in our lab. We occupy approximately 2,000 sqaure feet of modern laboratory space in the Stellar-Chance Laboratories

Instrumentation includes a number of environmental shakers, fermentors, cell disrupters, high and ultra-high speed centrifuges and several AKTA high performance liquid chromatography systems. In addition, we have analytical electrophoresis, Varian UV/Vis and FluoroLog fluorescence spectrometers, PCR thermocylcers, and an AutoLab electrochemical workstation.

 

Downstairs

We are fortunate to largely dominate three high performance Bruker Avance III NMR spectrometers. Housed in Blockley Hall, adjacent to the Stellar-Chance molecular biology laboratory, the instruments are clustered around the main office area where most of the Wand research group resides. Operating at 750 (shown), 600 and 500 MHz (1H), each spectrometer is equipped with a state of-the-art cryoprobes. New consoles and cryoprobes were installed in 2008-2009.

The Wand lab is noted for its use of novel approaches to using NMR to address fundamental problems in protein biophysics. These include high pressure NMR and reverse micelle encapsulation. To facilitate this we maintain a small machining capability where we have developed much of the gear used. Professor Wand and two former postdocs, Ron Peterson & Brian Lefebvre, have spun off this technology to form a small company, Daedalus Innovations.

 

 

Each member of the Wand lab has a dedicated Linux workstation for data processing. The computer/office area underwent a "refreshment" in the Summer of 2009. Graduate students and postdocs are actively engaged in the support of the instruments and learn to manage and trouble-shoot spectrometers during their stay with us providing a foundation for supporting instruments on their own.