Donald L. Siegel

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Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
Attending Physician, Dept. Pathology & Lab Med, Penn-Presbyterian Medical Center
Director, ACGME-accredited Fellowship Program in Blood Banking/Transfusion Medicine, Hospital of the University of PA
Medical Director, AABB-accredited Blood Bank and Apheresis Unit, Hospital of the University of Pennsylvania
Member for Oversight of Blood Usage, Clinical Effectiveness and Quality Improvement Committee, Hospital of the University of PA
Medical Director, FACT-accredited cell collection and processing facilities for Hematopoietic Stem Cell Transplant Program, Hospital of the University of Pennsylvania
Member, Executive Committee, Dept of Pathology & Lab Med, University of Pennsylvania School of Medicine
Attending Physician, Division of Transfusion Med, Dept. Pathology & Lab Med, Hospital of the University of PA
Founding Director, Division of Transfusion Medicine & Therapeutic Pathology, Hospital of the University of PA
Member, Cell & Vaccine Production Facility Advisory Committee, University of Pennsylvania School of Medicine
Medical Director, FACT-accredited Clinical Cell Vaccine and Production Facility, University of Pennsylvania
Medical Director, Penn Medicine Blood Donation Center, Hospital of the University of PA
Medical Director for Cellular Therapies, Penn Medicine
Department: Pathology and Laboratory Medicine

Contact information
510 Stellar-Chance Laboratories
422 Curie Blvd.
Philadelphia, PA 19104
Office: (215) 898-9655
Education:
Sc.B. (Biophysics)
Brown University, 1977.
Ph.D. (Biophysics)
Harvard University, 1983.
M.D.
University of Pennsylvania, 1987.
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Description of Research Expertise

Research Interests

Production and characterization of pathogenic human monoclonal auto- and alloantibodies

Research Summary

My laboratory is interested in characterizing the human immune response to auto- and alloantigens on a molecular level in order to understand more about human immune repertoire development. Our goal is to use this information to develop specific therapeutic agents that down-regulate immune responses in the settings of autoimmune disease such as immune hemolytic anemias and thrombocytopenias, transfusion reactions, hemolytic disease of the newborn, and acute rejection of solid organ allografts.
To approach these problems experimentally, we have combined Fab/phage-display technology with a novel cell-surface panning method to isolate large arrays of clinically significant human monoclonal antibodies from individual patients. This approach has enabled us to study the genetic and immunological properties of pathogenic auto- and alloantibodies.

Description of Itmat Expertise

Dr. Siegel's research focuses on the study of the molecular genetics of human auto- and alloantibodies responsible for blood cell-related disorders such as immune cytopenias (ITP, AIHA, TTP), hemolytic transfusion reactions, and hemolytic disease of the newborn. The Siegel laboratory has developed several phage display-related technologies for the cloning of human immune repertoires and the production of human monoclonal antibodies. These studies have led to the development of novel diagnostic reagents and antibodies with therapeutic potential.

Selected Publications

Villa C., Pan D., Zaitsev S., Siegel D.L., Muzykantov, V.: Coupling drugs to carrier erythrocytes: A resurrection of an (almost) forgotten approach for vascular drug delivery. Therapeutic Delivery in press 2015.

Rapoport, A.P., Stadtmauer, E.A., Binder-Scholl, G.K., Goloubeva, O., Vogl, D.Y., Lacey, S., Badros, A.Z., Garfall, A., Weiss, B., Finklestein, J., Kulikovskaya, I., Sinha, S.K., Kronsberg, S., Gupta, M., Bond, S., Melchiori, L., Brewer, J.E., Bennett, A.D., Gerry, A.B., Pumphrey, N.J., Williams, D., Tayton-Martin, H.K., Ribeiro, L., Holdich, T., Yanovich, S., Hardy, N., Yared, J., Kerr, N., Philip, S., Westphal, S., Siegel, D.L., Levine, B.L., Jakobsen, B.K., Kalos, M., June, C.H.: NY-ESO-1 specific engineered T-cells mediate sustained antigen-specific antitumor effects in myeloma. Nature Medicine 10.1038/nm.3910, 2015.

Pickens, B., Mao, Y., Li, D., Siegel, D.L., Poncz, M., Cines, D.B., Zheng, X.L.: Platelet-delivered ADAMTS13 protects against arterial thrombosis and antibody-mediated acquired thrombotic thrombocytopenic purpura. Blood 10.1038/nm.3910, 2015.

Casina, V.C., Hu, W., Mao, J., Lu, R.-N., Hanby, H.A., Pickens, B., Kan, Z., Lim, W.K., Mayne, L., Ostertag, E., Kacir, S., Siegel, D.L., Englander, S.W., Zheng, X.L.: High resolution epitope mapping by HX MS reveals the pathogenic mechanism and possible therapy for autoimmune TTP disease. Proc Natl Acad Sci (USA) doi: 10.1073/pnas.1512561112, 2015.

Siegel D.L., Westhoff, C: Rh and LW Blood Groups. Rossi’s Principles of Transfusion Medicine. Simon T.L., Snyder E.L., Stowell C.P., Strauss R.G. (eds.). Wiley-Blackwell, in press 2015.

Hammers, C., Chen, J., Lin, C., Kacir, S., Siegel, D.L., Payne, A.S., Stanley, J.R.: Persistence of anti-desmoglein 3 IgG+ B-cell clones in pemphigus patients over years. J Invest Dermatol 135: 742-749, 2015.

Gao, C., Liu, Y., Zhang, H., Zhang, Y., Fukuda, M.N., Palma, A.S., Kozak, R.P., Childs, R.A., Nonaka, M., Li, Z., Siegel, D.L., Hanfland, P., Peehl, D.M., Chai, W., Greene, M.I., Feizi, T.: Carbohydrate sequence of the prostate cancer-associated antigen F77 assigned by a mucin O-glycome designer array. J. Biol. Chem. 289: 16462-16477, 2014.

O'Doherty, U., Siegel, D.L.: Transfusion Reactions. The Intensive Care Manual, 2nd edition. Lanken, P.N., Manaker S., Kohl B.A., Hanson C.W. (eds.). Elsevier Saunders, Philadelphia, Page: 450-456, 2014.

Zhao A., Tohidkia M.R., Siegel D.L., Coukos G., Omidi Y.: Phage antibody display libraries: a powerful antibody discovery platform for immunotherapy. Critical Reviews in Biotechnology 10.3109/07388551.2014.958978: 1-14, 2014.

Azzato E., Gentile C., Carpenter H., Siegel D., Watt C.: Successful red blood cell gemotyping from leukoreduced packed red blood cell segments. abstracts of the 2014 Annual Meeting of the Association for Molecular Pathology 2014.

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Last updated: 07/23/2015
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