415 Curie Blvd.
Research Interest
I am interested in the genetic basis of phenotypic differences across human populations, in particular the skeletal and cardiac traits. I study the role of population-specific genetic variants in heart, muscle and bone development using population genetic and experimental methods. My research will employ human induced pluripotent stem cells (hiPSCs) of diverse genetic backgrounds as a model and differentiate them into specific lineages to understand how genetic (coding and noncoding) variations influence human phenotypes.
Education
2015 2010 | Ph.D. Molecular Medicine, Peking University, Institute of Molecular Medicine, Beijing, China B.S. Biological Science, Department of Life Science, Sichuan University, Chengdu, China |
Research Experience
2021 – Present 2019 - 2021 2015–2019 | Research Scientist Research Associate Postdoctoral Associate |
Publications
Feng Y, Xie N, Inoue F, Fan S, Saskin J, Zhang C, Zhang F, Hansen M, Nyambo T, Mpoloka SW, Mokone GG, Fokunang C, Belay G, Njamnshi AK, Marks MS, Oancea E, Ahituv N, Tishkoff SA. Integrative functional genomic analyses identify novel genetic variants influencing skin pigmentation in indigenous Africans. Nature Genetics. 2024 (IF 41.3, accepted)
Li Z, Dai R, Chen M, Huang L, Zhu K, Li M, Zhu W, Li Y, Xie N, Li J, Wang L, Lan F, Cao CM. p55γ degrades RIP3 via MG53 to suppress ischaemia-induced myocardial necroptosis and mediates cardioprotection of preconditioning. Cardiovascular Research. 2023, 119(14), 2421-2440 (IF 13.1)
Xie N, Chu SN, Schultz C, Chan SS. Efficient muscle regeneration by human PSC-derived CD82+ERBB3+NGFR+ skeletal myogenic progenitors. Cells. 2023, 12(3):362 (IF 7.6).
Xie N, Chan SS. Producing engraftable skeletal myogenic progenitors from pluripotent stem cells via teratoma formation. Methods in Molecular Biology. 2023, 2640:175-189.
Pappas MP*, Xie N*, Penaloza JS, Chan SS. Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived Teratomas. Cells. 2022, 11(9): 1589 (*co-first author, IF 7.6).
Xie N, Chu SN, Azzag K, Schultz CB, Peifer LN, Kyba M, Perlingeiro R, Chan SS. In vitro expanded skeletal myogenic progenitors from pluripotent stem cell-derived teratomas have high engraftment capacity. Stem Cell Reports. 2021 (IF 7.765. Accepted)
Xie N, Chan SS. Producing engraftable skeletal myogenic progenitors from pluripotent stem cells via teratoma formation. Methods in Molecular Biology. 2021 (Accepted)
Li Z, Dai R, Chen M, Huang L, Zhu W, Zhu K, Li Z, Xie N, Guo Y, Ni X, Cao CM. Preconditioning increases p55γ and degrades RIP3/RIP1 via MG53 to prevent ischemia-induced myocardial necroptosis. (Under revision)
Feng Y*, Xu H*, Liu J*, Xie N, Gao L, He Y, Yao Y, Lv F, Zhang Y, Lu J, Zhang W, Li CY, Hu X, Yang Z, Xiao RP. Functional and Adaptive Significance of Promoter Mutations that Affect Divergent Myocardial Expressions of TRIM72 in Primates. Molecular Biology and Evolution. 2021, 38, 2930-2945
Goloviznina NA, Xie N, Dandapat A, Iaizzo PA, Kyba M. Prospective isolation of human fibroadipogenic progenitors with CD73. Heliyon. 2020, 6(7), e04503.
Collins BC, Arpke RW, Larson AA, Baumann CW, Xie N, Cabelka CA, Nash NL, Juppi HK, Laakkonen EK, Sipilä S, Kovanen V, Spangenburg EE, Kyba M, Lowe DA. Estrogen Regulates the Satellite Cell Compartment in Females. Cell Reports. 2019, 28(2): 368-381 (IF 8.032) LINK
Penaloza J, Pappas MP, Hagen HR, Xie N, Chan SS. Single-cell RNA-seq analysis of Mesp1-induced skeletal myogenic development. Biochemical and biophysical research communications. 2019, 520, 284-290
Chan SS, Arpke RW, Filerato A, Xie N, Pappas MP, Penaloza J, Perlingeiro R, Kyba M. Skeletal muscle stem cells from PSC-derived teratomas have functional regenerative capacity. Cell Stem Cell. 2018, 23(1):74-85
Xie N*, Chen M*, Dai R, Zhang Y, Zhao H, Song Z, Zhang L, Li Z, Feng Y, Gao H, Wang L, Zhang T, Xiao RP, Cao CM. SRSF1 promotes vascular smooth muscle cell proliferation through a Δ133p53/EGR1/KLF5 pathway. Nature Communications. 2017, 8:16016
Li G*, Xie N*, Yao Y, Zhang Y, Guo J, Feng Y, Lv F, Xiao RP, Cao CM. Identification of p55γ as a novel inhibitor of vascular smooth muscle cell proliferation and neointimal formation. Cardiovascular Research. 2015, 105(1):75. (*co-first author, IF 5.878)
Guo J, Xie N, Li G, Zhang Y, Lv F, Guo S, Feng Y, Cao CM, Xiao RP. p55γ functional mimetic peptide N24 blocks vascular proliferative disorders. Journal of Molecular Medicine, 2015, 93(10):1107-1118. (IF 4.686)
Guo J, Xiang W, Zhang G, Luo T, Xie N, Yang Z, Sun Q. Mycobacterial interspersed repetitive unit typing in Mycobacterium tuberculosis isolates from Sichuan province in China. The Indian Journal of Medical Research, 2011, 134(3):362. (IF 1.532)
Patents
Kyba M, Natalya G, Xie N, Abhijit D (2021). Methods for producing fibroadipogenic progenitor cells. US20210388321A1
Kyba M, Goloviznina N, Dandapat A, Xie N (2019). Surface markers for identification and purification of fibroadipogenic progenitor cells from human skeletal muscle. UMN Case #2020-195
Kyba M, Xie N, Chan SS (2016). Surface markers for identification and purification of skeletal muscle stem cells. US provisional patent: 600.A14PRV
Invited Lectures
Xie N (2021). Expansion of engraftable skeletal myogenic progenitors from pluripotent stem cell-derived teratomas. Pediatric Research, Education and Scholarship Symposium, Department of Pediatrics, University of Minnesota (35th Annual PRESS Award)
Xie N (2020). Making muscles from human PSC-derived teratomas: a novel in vivo differentiation strategy. Stem Cell Institute Research Conference, Stem Cell Institute, University of Minnesota
Xie N (2018). Aryl hydrocarbon receptor is necessary for self-renewal and expansion of adult muscle stem cells. Lillehei Heart Institute Research Seminar Series, Lillehei Heart Institute, University of Minnesota
Xie N (2014). Identification of PI3K regulatory subunit p55 as a novel inhibitor of VSMC proliferation and neointimal formation. Annual Meeting of State Key Laboratory of Biomembrane and Membrane Biotechnology, Peking University, Beijing, China
Selected Abstract
Xie N, Feng Y, Tishkoff SA (2023). Genetic variation in a distal enhancer modifies cardiac transcription factor HAND2 expression and function. ASHG (American Society of Human Genetics) 2023 Annual Meeting. Washington, DC, USA
Xie N, Feng Y, Fan S, et al. (2022). Identifying functional variants related to heart development in African populations. ASHG (American Society of Human Genetics) 2022 Annual Meeting. Los Angeles, CA, USA (Reviewers’ Choice Abstracts, TOP10%)
Xie N, Feng Y, Kelly DE, Zhang F, Sun Y, Ye CJ, Prak ETL, Tishkoff SA (2022). Investigating the peripheral immune response in ethnically diverse Africans by single-cell RNA-seq. CZI (Chan Zuckerberg Initiative) Single-Cell Biology Annual Meeting. San Jose, CA, USA
Xie N, Chu S, Peifer L, Kyba M, Chan SS (2021). Skeletal myogenic progenitors from pluripotent stem cell-derived teratomas possess high engraftment potential after in vitro expansion. Stem Cell Institute/Engineering symposium. University of Minnesota
Xie N, Peifer L, Chu S, Kyba M, Chan SS (2020). Producing skeletal myogenic progenitors from human PSC-derived teratomas. Rare Disease Day 2020. University of Minnesota
Xie N, Chu S, Lang H, Chan SS, Kyba M (2019). Aryl hydrocarbon receptor regulates expansion and self-renewal of satellite cells. Cardio Palooza 11: Annual Cardiovascular Retreat, University of Minnesota
Xie N, Chan SS, Arpke RW, Chu S, Kyba M (2018). Study on aryl hydrocarbon receptor in satellite cells in response to muscle injury. Greg Marzolf Jr. Symposium, University of Minnesota
Xie N, Chan SS, Arpke RW, Kyba M (2017). Aryl hydrocarbon receptor is a regulator of satellite cell expansion in adult mice. Cardio Palooza 9: Annual Cardiovascular Retreat, University of Minnesota (Best Poster Award)
Xie N, Yao Y, Zhang Y, Cao CM, Xiao RP (2014). p55 suppresses VSMC proliferation and neointimal formation. American Heart Association 2014 Scientific Sessions - Basic Cardiovascular Sciences, Las Vegas, Nevada, USA
Awards and Honors
University of Pennsylvania
- 2022 Reviewers’ Choice Abstracts, ASHG 2022 Annual Meeting
University of Minnesota
- 2021 35th Annual PRESS Award
- 2017 9th Annual Cardio Palooza Best Poster Award
Peking University
- 2013 Presidential Scholarship
- 2012 Guanghua Scholarship
- 2010-2015 Academy Scholarship
Sichuan University
- 2010 Honor for Excellent Bachelor Degree Dissertation
- 2007, 2009 National Scholarship, the Ministry of Education of China
- 2007-2009 Honor for Excellent Undergraduate
