Luis J. Montaner

faculty photo
Wistar Institute Professor of Medicine
Department: Medicine
Graduate Group Affiliations

Contact information
The Wistar Institute
3601 Spruce Street
Philadelphia, PA 19104
Office: 215-898-9143
Fax: 215-573-7008
Lab: 215-898-3934
Education:
B.S.
Kansas State University, 1989.
M.Sc. (Veterinary Pathology)
Kansas State University, 1991.
D.V.M. (Veterinary Medicine)
Kansas State University, 1991.
D.Phil. (Experimental Pathology)
University of Oxford, 1995.
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Description of Research Expertise

Research Interests
Our goal is to develop a better understanding of HIV-1 immunopathogenesis and new immune-based strategies of anti-HIV therapy that are better tolerated and more sustainable for patient populations than the life-long use of antiretroviral therapy.

Research Description
Introduction

The Montaner laboratory is investigating mechanisms of disease in HIV-1 infection and novel approaches to augment immune function by combining virological and immune-based research on patient-derived material as well as by using laboratory models of virus infection. The work is focused on 1) regulation of innate immunity, 2) identifying new mechanisms of immunodeficiency and discovering new approaches to reverse them, 3) exploring new therapy management practices, and 4) understanding the relationship between immune antiviral responses and control of HIV-1 infection.

Current Research Activities and Interests

Innate Immunity & HIV-1 Infection: Dendritic cells & Natural Killer Cells Direct or indirect interactions of viral particles with innate and specific adaptive immunity effector cells affects the cross talk between antigen presenting cells (APCs), NK cells and the antigen specific T and B-lymphocytes, and may contribute to regulate HIV disease progression. Specifically, we are pursuing analysis of the effects of HIV infection in macrophages, dendritic cells and Natural Killer cells.

A relationship between levels of HIV replication and innate cell function is supported by our preliminary data on DC and NK subset changes and viral replication in HIV-infected individuals showing an impairment of NK cell responses, APC endocytic uptake, differential expression of cell surface molecules associated with APC function, increased APC apoptosis, decreased IL-12 secretion, decreased IFN-? secretion and a loss of plasmacytoid dendritic cells (PDCs) and myeloid dendritic cells (MDCs) in PBMC. Based on these observations and the observed effects of antiretroviral therapy on DC and NK cell subsets, the inverse correlation between viral load and DC subsets in untreated HIV positive subjects and our observations of augmented NK lytic activity by activated DC, we are addressing longitudinal analysis and mechanistic experiments on DC/HIV interactions to test the hypothesis that HAART-mediated viral suppression restores mature NK and DC subsets necessary to activate innate mechanisms of antiviral control through lysis of infected cells. The long-term goal of this area of focus is to define the contribution of two major components of the innate immune system (accessory and Natural Killer cells) in controlling HIV replication thereby modifying disease progression. The short-term goal of our effort is to address the consequences of immune reconstitution on innate immunity following antiretroviral therapy, with particular emphasis on correlates of DC and NK cell functions and the consequences of HIV interactions with DC subsets. While adaptive HIV-specific immune responses continue to be an area of active investigation in AIDS research, the potential contribution of innate immune response, such as the relationship between DC subsets, disease progression and its consequences on other innate functions such as NK function remains largely unexplored in HAART settings. This study represents a hypothesis-driven collaborative effort by The Wistar Institute, the Infectious Disease Division of the University of Pennsylvania Hospital, Philadelphia FIGHT, Schering-Plough, Becton Dickinson, The Women's Interagency Study Cohort and the Multiple AIDS Cohort Study (MACS).

Selected Publications

Mullin, J.M., Diguilio, K.M., Valenzano, M.C., Deis, R., Thomas, S., Zurbach, E.P., Abdulhaqq, S., Montaner, L.J. : Zinc reduces epithelial barrier compromise induced by human seminal plasma. PLOS One March 2017.

Tomescu, C., Tebas, P, Montaner, L.J.: IFN- Augments NK-mediated Antibody-Dependent Cellular Cytotoxicity (ADCC) of HIV-1 infected autologous CD4+ T Cells regardless of MHC-I downregulation. AIDS January 2017.

Riley, J.L. & Montaner, L.J. : Cell-mediated immunity to target the persistent HIV reservoir. Journal of Infectious Diseases 2017.

Amet, T., Son, Y.M., Jiang, L., Cheon, I.S., Huang, S., Gupta, S.K., Dent, A.L., Montaner, L.J., Sun, J. : BCL6 Represses intrinsic antiviral resistance in follicular T helper cells. Leukoc Biol. 2017.

Sterman, D.H., Stevenson, J., Friedberg, J., Metzger, S., Recio, A., Moon, E., Haas, A.R., Vachani, A., Katz, S.I., Sun, J., Heitjan, D.F., Hwang, W.-T., Litzky, L., Yearley, J.H., Tan, K.S., Papasavvas, E., Kennedy, P., Montaner, L.J., Cengel, K., Simone, C.B. II., Culligan, M., Langer, C.J., Albelda, S.M: A pilot and feasibility clinical trial evaluating immuno-gene therapy of malignant pleural mesothelioma (MPM) using intrapleural delivery of adenovirus- interferon-alpha (AD.hIFN-α2b) in combination with high-dose celecoxib and systemic chemotherapy. Clinical Cancer Research August 2016.

Abdulhaqq, S.A., Zorilla, C., Kang, G., Yin, X., Tamayo, V., Seaton, K., Joseph, J., Garced, S., Sariol, C.A., Tomaras, G., Foulkes, A.S., VerMilyea, M., Coutifaris, C., Kossenkov, A., Kraiselburd, E.N., Li, Q., Montaner, L.J. : Long-term HIV seronegative female sex workers sustain high cervical IFNε, low mucosal/systemic immune activation and low gene expression required for HIV infection. Mucosal Immunology. Mucosal Immunology July 2016.

Papasavvas, E., Surrey, L.F., Glencross, D.K., Azzoni, L., Joseph, J., Omar, T., Feldman, M.D., Williamson, A.-L., Siminya, M., Swarts, A., Yin, X., Liu, Q., Firnhaber, C., Montaner, L.J. : High-risk oncogenic HPV genotype infection associates with increased immune activation and T cell exhaustion in ART-suppressed HIV-1 infected women. Oncoimmunology May 2016.

Morón-Lopez, S., Gómez-Mora, E., Salgado, M., Ouchi, D., Muertas, M.C., Urrea, V., Pérez, M., Tural, C., Clotet, B., Montaner, L.J., Blanco, J., Crespo, M., and Martinez-Picado, J: Short-term treatment with IFNα diminishes expression of HIV-1 and reduces CD4+ T-cell activation in HIV/HCV-co-infected patients on antiretroviral therapy. J Infect Dis. 2016.

Patro, S.C., Azzoni, L., Joseph, J., Fair, M.G., Sierra-Madero, J.G., Rassool, M.S., Sanne, I., Montaner, L.J.: Antiretroviral therapy in HIV-1 infected individuals with CD4 count below 100 cells/mm3 results in differential recovery of monocyte activation. J Leukoc Biology 2016.

Tomescu, C., Mavilio, D. and Montaner, L.J.: Lysis of HIV-1 infected autologous CD4+ primary t cells by interferon-alpha activated NK cells requires NKp46 and NKG2D. AIDS 2015.

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Last updated: 08/25/2017
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