Sita Awasthi

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Research Assistant Professor of Medicine
Department: Medicine

Contact information
522F Johnson Pavilion
3610 Hamilton walk
Philadelphia, PA 19104
Office: 215-573-8422
Fax: 215-349-5111
Lab: 215-573-8444
BS (Biology and Chemistry )
Devi Ahilya University, Indore, India , 1982.
MS (Biochemistry)
Devi Ahilya University, Indore, India , 1984.
PhD (Biochemistry)
Devi Ahilya University, Indore, India , 1989.
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Description of Research Expertise

Microbiology and Immunology
•General virology techniques including, production, purification and titration, strategy to create mutant viruses, making pseudo viruses, testing, etc.
•General immunological techniques for example antibody response using ELISA, neutralization assay, binding assays, rosetting assay for IgG binding, FACS analysis.
•Tissue and cell culture techniques, maintenance of cell lines, Transfection studies, Phase contrast, Immuno-fluorescent and confocal-microscopy.
•Developed animal models and experienced in working with infectious agents in humanized mouse model.

Protein Chemistry/ Proteomics
•Protein purification using affinity chromatography and HPLC.
•Recombinant protein expression in E. coli, insect cells and yeast, Structure-function relationship.
•Protein electrophoresis, 2-Dimentional gel electrophoretic analysis, protein-protein interactions, protein phosphorylation, phosphoaminoacid analysis.
•Production and characterization of monoclonal and polyclonal antibodies.
•Hands on experience with GCG as well as new bioinformatics programs for computational analysis of proteins to predict liner functional motif or structure.

Molecular and Cellular Biology
•Cloning strategy, Northern and Southern blotting, Invirto transcription and translation, RNA manipulation, Protein-RNA interactions, Site directed mutagenesis, RT-PCR quantitation, Gel shift assay, RNAse protection, In situ hybridization, Micro array, Two-hybrid assay.
•Knowledge of signaling pathways induced under stress conditions and their assays.
•Hands on experience with GCG as well as new Bioinformatics program for computational analysis of DNA and RNA. I submitted three new splice variants m-RNA sequence on gene bank.

General Lab practices
•Hands on experience with medical, pharmaceutical, clinical and basic science research information retrieval from various data bases.
•Trained for handling radioactivity.
•Knowledge of GLP and good record keeping.
•Supervised technical staff and undergraduate and graduate students at University of Pennsylvania and other institutions.
•Experienced in writing animal protocols for approval from IUCAC.

Selected Publications

Awasthi S, Friedman HM: Status of prophylactic and therapeutic genital herpes vaccines. Current Opinion in Virology 6: (In press), 2014.

Sita Awasthi: Immune Evasion by Persistent Viruses and Cancers: Blocking Evasion as a Rational Design to Treat Viral Infections and Cancers. Journal of Antivirals and antiretrovirals 3(5), November 2013.

Awasthi S., and Friedman, H.: A Paradigm Shift: Vaccine-Induced Antibodies as an Immune Correlate of Protection against Herpes Simplex Virus Type 1 Genital Herpes Journal of Infectious Disease November 2013 Notes: Epub ahead of print.

Kamoun M., Filali M., Murray M.V., Awasthi S., Wadzinski B. E. : Protein phosphatase 2A family members (PP2A and PP6) associate with U1 snRNP and the spliceosome during pre-mRNA splicing. Biochemical and Biophysical Research Communications 440 (2): 306-311, October 2013.

Awasthi S., Huang J., Shaw C.E. and Friedman H.: A Trivalent Subunit Glycoprotein Vaccine for Prevention of Genital Herpes. International Herpes Workshop in Grand Rapids, Michigan, USA. July 2013.

Awasthi S., Shaw C. E., Huang J., Friedman H. M.: A Trivalent Subunit Glycoprotein Vaccine for Prevention of Genital Herpes disease. Department of Medicine Research June 2013.

Awasthi S., Balliet J.W., Flynn J A , Lubinski J M , Shaw C E, Distefano D J, Cai M, Brown M, Smith J F, Kowalski R, Swoyer R, Galli J, Copeland V, Rios S, Davidson R C, Salnikova M, Kingsley S , Bryan J, Casimiro D R and Friedman H M. : Protection Provided by an HSV-2 Glycoprotein C and D Subunit Antigen Vaccine against Genital HSV-2 Infection in HSV-1 Seropositive Guinea Pigs. Journal of Virology 2013 Notes: epub ahead of print.

Shedlock DJ, Talbott KT, Wu SJ, Wilson CM, Muthumani K, Boyer JD, Sardesai NY, Awasthi S, Weiner DB: Vaccination with synthetic constructs expressing cytomegalovirus immunogens is highly T cell immunogenic in mice. Human Vaccines & Immunotherapeutics 8(11): 1-14, November 2012.

Awasthi S, Lubinski JM, Shaw CE, Cai M, Friedman HM : Protection by a gD2&gC2 glycoprotein subunit vaccine against HSV-2 acute and recurrent infection in HSV-1 seropositive guinea pigs. International Herpesvirus Workshop, Calgary, Canada 35, Aug 2012 Notes: Poster presentation.

Awasthi S, Bao H, Muthumani K, Friedman HM : Humanized mouse model to study viral infections and evaluate antivirals. 2nd World Congress on Virology, Las Vegas, NV Aug 2012 Notes: Oral presentation.

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Last updated: 03/27/2014
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