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Donald L. Siegel, MD, PhD

Donald L. Siegel, MD, PhD

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Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
Department: Pathology and Laboratory Medicine

Contact information
510 Stellar-Chance Laboratories
422 Curie Blvd.
Philadelphia, PA 19104
Office: (215) 898-9655
Sc.B. (Biophysics)
Brown University, 1977.
Ph.D. (Biophysics)
Harvard University, 1983.
University of Pennsylvania, 1987.
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Perelman School of Medicine > Faculty > Search

Description of Research Expertise

Research Interests

Production and characterization of pathogenic human monoclonal auto- and alloantibodies

Research Summary

My laboratory is interested in characterizing the human immune response to auto- and alloantigens on a molecular level in order to understand more about human immune repertoire development. Our goal is to use this information to develop specific therapeutic agents that down-regulate immune responses in the settings of autoimmune disease such as immune hemolytic anemias and thrombocytopenias, transfusion reactions, hemolytic disease of the newborn, and acute rejection of solid organ allografts.
To approach these problems experimentally, we have combined Fab/phage-display technology with a novel cell-surface panning method to isolate large arrays of clinically significant human monoclonal antibodies from individual patients. This approach has enabled us to study the genetic and immunological properties of pathogenic auto- and alloantibodies.

Description of Clinical Expertise

transfusion medicine, blood banking, apheresis, hematopoietic stem cell collection/processing

Selected Publications

Ostertag, E.M., Bdeir, K., Kacir, S., Thiboutot, M., Gulendran, G., Yunk, L., Hayes, V.M., Motto, D.G., Poncz, M., Zheng, X.L., Cines, D.B., Siegel, D.L.: ADAMTS13 Autoantibodies Cloned from Patients with Acquired Thrombotic Thrombocytopenic Purpura: 2. Pathogenicity in an animal model. Transfusion 56: 1775-1785, 2016.

Bhoj V., Nunez-Cruz S., Feldman M.D., Mansfield K., Rader C., Siegel D.L., Milone M.C.: Immunotherapy of medullary thyroid carcinoma by GDNF family receptor alpha 4 (GFRalpha4)-targeted adoptive T cell immunotherapy. American Association for Cancer Research 2016 Annual Meeting, New Orleans, LA. 2016.

Fesnak, A., Chie, Y.L., Siegel, D.L., Maus, M.: CAR-T cell therapies from the transfusion medicine perspective. Transfusion Medicine Reviews 30: 139-145, 2016.

Fuentes, R.E., Zaytsev, S., Sook Ahn, H., Hayes, V., Kowalska, M.A., Lambert, M.P., Wang, Y., Siegel, D.L., Bougie, D.W., Aster, R.H., Myers, D.D., Stepanova, V., Cines, D.B., Muzykantov, V.R., Poncz, M.: A chimeric platelet-targeted urokinase prodrug selectively blocks new thrombus formation. J Clin Invest 126: 483-494, 2016.

Mei J., Qing D., Hotz M., Siegel D.L., Sondheimer N., Mangalmurti N.S.: Mitochondrial DNA released following necroptosis accumulates on RBCs. American Thoracic Society 2016 Conference, San Francisco, CA 2016.

Siegel D.L., Westhoff, C: Rh and LW Blood Groups. Rossi’s Principles of Transfusion Medicine. Simon T.L., Snyder E.L., Stowell C.P., Strauss R.G. (eds.). Wiley-Blackwell, 2016.

Zhao A., Tohidkia M.R., Siegel D.L., Coukos G., Omidi Y.: Phage antibody display libraries: a powerful antibody discovery platform for immunotherapy. Critical Reviews in Biotechnology 36: 276-289, 2016.

Ostertag, E.M., Kacir, S., Thiboutot, M., Gulendran, G., Zheng, X.L., Cines, D.B., Siegel, D.L.: ADAMTS13 Autoantibodies Cloned from Patients with Acquired Thrombotic Thrombocytopenic Purpura: 1. Structural and functional characterization in vitro. Transfusion 56: 1763-1774, 2016.

Villa CH., Pan DC., Zaitsev S., Cines D.B., Siegel D.L., Muzykantov, V.R.: Delivery of drugs bound to erythrocytes: new avenues for an old intravascular carrier. Therapeutic Delivery 6: 795-826, 2015.

Hammers, C.M., Chen, J., Lin, C., Kacir, S., Siegel, D.L., Payne, A.S., Stanley, J.R.: Persistence of anti-desmoglein 3 IgG+ B-cell clones in pemphigus patients over years. J Invest Dermatol 135: 742-749, 2015.

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Last updated: 07/27/2016
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