Donald L. Siegel, MD, PhD

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Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
Department: Pathology and Laboratory Medicine

Contact information
9-112 South Tower, Big 421
3400 Civic Center Blvd
Philadelphia, PA 19104
Office: (215) 662-3942
Lab: (215) 898-9655
Education:
Sc.B. (Biophysics)
Brown University, 1977.
Ph.D. (Biophysics)
Harvard University, 1983.
M.D.
University of Pennsylvania, 1987.
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Description of Research Expertise

Research Interests

Production and characterization of pathogenic human monoclonal auto- and alloantibodies

Research Summary

My laboratory is interested in characterizing the human immune response to auto- and alloantigens on a molecular level in order to understand more about human immune repertoire development. Our goal is to use this information to develop specific therapeutic agents that down-regulate immune responses in the settings of autoimmune disease such as immune hemolytic anemias and thrombocytopenias, transfusion reactions, hemolytic disease of the newborn, and acute rejection of solid organ allografts.
To approach these problems experimentally, we have combined Fab/phage-display technology with a novel cell-surface panning method to isolate large arrays of clinically significant human monoclonal antibodies from individual patients. This approach has enabled us to study the genetic and immunological properties of pathogenic auto- and alloantibodies.

Description of Clinical Expertise

transfusion medicine, blood banking, apheresis, hematopoietic stem cell collection/processing

Selected Publications

Chen, J., Zheng, Q., Hammers, C.M., Ellebrecht, C., Mukherjee, C., Tang, H-Y., Lin, C., Yuan, H-J., Pan, M., Langenhan, J., Komorowski, L., Siegel, D.L., Payne, A.S., Stanley, J.R.: Proteomic analysis of pemphigus autoantibodies indicates a much larger, more diverse, and dynamic repertoire than determined by B-cell genetics. Cell Reports 18: 237-247, 2017.

Villa C.H., Cines D.B., Siegel D.L., Muzykantov V.R.: Erythrocytes as carriers for drug delivery in blood transfusion and beyond. Transfusion Medicine Reviews 31: 26-35, 2017.

Villa, C.H., Pan, D.C., Johnston, I., Greineder, C.F., Walsh, L., Hood, E., Cines, D.B., Poncz, M., Siegel, D.L., Muzykantov, V.R.: Biocompatible coupling of therapeutic fusion proteins to human erythrocytes. Blood Advances in press, 2017.

Fraietta J.A., Lacey S.F., Orlando E.J., Pruteanu-Malinici I., Gohil M., Lundh S., Boesteanu A.C., Wang Y., O’Connor R.S., Hwang W.-T., Pequignot E., Ambrose D.E., Zhang C., Wilcox N., Bedoya F., Dorfmeir C., Chen F., Tian L., Parakandi H., Gupta M., Young R.M., Johnson F.B., Kulikovskaya I., Liu L., Xu J., Kassim S.H., Suhoski-Davis M.M., Levine B.L., Frey N.V., Siegel D.L., Huang A.C., Wherry E.J., Bitter H., Brogdon J.L., Porter D.L., June C.H., Melenhorst J.J.: Identification of functional determinants of response and resistance to CD19 chimeric antigen receptor (CAR) T cell therapy of chronic lymphocytic leukemia. Blood 2017.

Carnemolla, R., Villa, C.H., Greineder, C.F., Zaitsev, S., Kowalska, A., Atochin, D.N., Cines, D.B., Siegel, D.L., Esmon, C.T. Muzykantov, V.R.: Targeting thrombomodulin to circulating red blood cells augments its protective effects in models of endotoxemia and ischemia-reperfusion injury. FASEB J 31: 761-770, 2017.

Beigel, J.H., Tebas, P., Elie-Turenne, Marie-Carmelle, et al: Immune plasma for the treatment of severe influenza: an open-label, multicenter, phase 2 randomised study. The Lancet Respiratory Medicine 5: 500-511, 2017.

Ostertag, E.M., Bdeir, K., Kacir, S., Thiboutot, M., Gulendran, G., Yunk, L., Hayes, V.M., Motto, D.G., Poncz, M., Zheng, X.L., Cines, D.B., Siegel, D.L.: ADAMTS13 Autoantibodies Cloned from Patients with Acquired Thrombotic Thrombocytopenic Purpura: 2. Pathogenicity in an animal model. Transfusion 56: 1775-1785, 2016.

Siegel D.L., Westhoff, C: Rh and LW Blood Groups. Rossi’s Principles of Transfusion Medicine. Simon T.L., McCullough J., Snyder E.L., Solheim B.G., Strauss R.G. (eds.). Wiley-Blackwell, 2016.

Zhao A., Tohidkia M.R., Siegel D.L., Coukos G., Omidi Y.: Phage antibody display libraries: a powerful antibody discovery platform for immunotherapy. Critical Reviews in Biotechnology 36: 276-289, 2016.

Ostertag, E.M., Kacir, S., Thiboutot, M., Gulendran, G., Zheng, X.L., Cines, D.B., Siegel, D.L.: ADAMTS13 Autoantibodies Cloned from Patients with Acquired Thrombotic Thrombocytopenic Purpura: 1. Structural and functional characterization in vitro. Transfusion 56: 1763-1774, 2016.

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Last updated: 12/01/2017
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