Donald L. Siegel, MD, PhD

faculty photo
Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
Department: Pathology and Laboratory Medicine

Contact information
9-112 South Tower, Big 421
3400 Civic Center Blvd
Philadelphia, PA 19104
Office: (215) 662-3942
Lab: (215) 898-9655
Sc.B. (Biophysics)
Brown University, 1977.
Ph.D. (Biophysics)
Harvard University, 1983.
University of Pennsylvania, 1987.
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Description of Research Expertise

Research Interests

Discovery and characterization of diagnostic and therapeutic monoclonal antibodies

Research Summary

My laboratory studies human immune repertoires in health and disease using phage display technologies for the production of human and non-human monoclonal antibodies relevant to transfusion medicine, benign hematology, infectious diseases, and oncology. Current studies focus on developing targeted drug delivery methods using red cell antibodies coupled to novel anti-thrombotic/anti-inflammatory molecules; cloning monoclonal primate neutralizing Dengue, Ebola, MERS and Zika virus antibodies for human in vivo antibody gene expression therapy; and isolating recombinant human and humanized antibody fragments for the design of T-cell chimeric antigen receptors for cancer therapy.

Description of Clinical Expertise

transfusion medicine, blood banking, apheresis, hematopoietic stem cell collection/processing, cGMP manufacture of cellular therapies for phase I first-in-human clinical trials

Selected Publications

Tebas, P., Jadlowsky, J.K., Shaw, P.A., Tian, L., Esparza, E., Brennan, A., Kim, S., Naing, S.Y., Richardson, M.W., Vogel, A.N., Maldini, C., Kong, H., Liu, X., Lacey, S.F., Bauer, A.M., Mampe, F., Lee, G., Dale Ando, D., Levine, B.D., Porter, D.L., Zhao, Y., Siegel, D.L., Bar, K.J., June, C.H., Riley, J.L.: CCR5-edited CD4 T cells augment HIV-specific immunity to enable post rebound control of HIV replication. J. Clin. Invest. Page: in press, 2021.

Mason, N.J., Chester, N., Xiong, A., Rotolo, A., Wu, Y., Glassman, P., Gulendan, G., Siegel, D.L.: Development of a fully canine anti-canine CTLA4 monoclonal antibody for comparative translational research in dogs with spontaneous tumors. mAbs 13: e2004638, 2021.

Melenhorst, J.J., Chen, G.M., Wang, M., Porter, D.L., Gao, P., Bandyopadhyay, S., Pruteanu-Malinici, I., Nobles, C.L., Maji, S., Frey, N.V., Gill, S.I., Tian, L., Kulikovskaya, I., Gupta, M., Davis, M.M., Fraietta, J.A., Brogdon, J.L., Young, R.M., Ambrose, D.E., Chew, A., Levine, B.L., Siegel, D.L., Alanio, C., Wherry, E.J., Bushman, F.D., Lacey, S.F., Tan,, K., June, C.H.: Decade-long remissions of leukemia sustained by the persistence of activated CD4+ CAR T-cell. Nature in press, 2021.

Tutwiler, V., Litvinov, R.I., Protopopova, A., Nagaswami, C., Villa, C., Abdulmalik, O., Siegel, D.L., Eric Russell, E.R., Muzykantov, V.R., Lam, W.A., Myers, D.R., Weisel, J.W.: Pathologically stiff erythrocytes can promote thrombosis by impeding contraction of blood clots. J. Thromb. Haemost. 19: 2894-2895, 2021.

Myers, R.M., Li, Y., Leahy, A.B., Barrett, D.M., Teachey, D.T., Callahan, C., Fasano, C.C., Rheingold, S.R., Amanda DiNofia, A., Wray, L. Aplenc, R., Baniewicz, D., Liu, H., Shaw, P.A., Pequignot, E., Getz, K.D., Brogdon, J.L., Fesnak, A.D., Siegel, D.L., Davis, M.M., Bartoszek, C., Lacey, S.F., Hexner, E.O., Chew, A., Wertheim, G.B., Levine, B.L., June, C.H., Grupp, S.A., Maude, S.L.: Humanized CD19-targeted Chimeric Antigen Receptor (CAR) T cells in CAR-naïve and CAR-exposed children and young adults with relapsed/refractory acute lymphoblastic leukemia. J. Clin. Oncol. 39: 3044-3055, 2021.

Halkidis, K., Siegel, D.L., Zheng, X.L.: A human monoclonal antibody against the C-terminus of ADAMTS13 modulates its susceptibility to an inhibitor in thrombotic thrombocytopenic purpura. J. Thromb. Haemost. 19: 1888-1895, 2021.

Bar, K.J., Shaw, P.A., Choi, G.H., Aqui, N., Fesnak, A., Yang, J.B., Soto-Calderon, H., Grajales, L., Starr, J., Andronov, M., Mastellone, M., Amonu, C., Feret, G., DeMarshall, M., Buchanan, M., Caturla, M., Gordon, J., Wanicur, A., Monroy, M.A., Mampe, F., Lindemuth, E., Gouma, S., Mullin, A.M., Barilla, H., Pronina, A., Irwin, L., Thomas, R., Eichinger, R.A., Demuth, F., Luning Prak, E.T., Pascual, J.L., Short, W., Elovitz, M.A., Baron, J., Meyer, N.J., Degnan, K.O., Frank, I., Hensley, S., Siegel, D.L., Tebas, P.: A randomized controlled study of convalescent plasma for individuals hospitalized with COVID-19 pneumonia. J. Clin. Invest. in press, 2021.

Bhoj, V., Li,, L., Parvathaneni, K., Zhang, Z., Kacir, S., Arhontoulis, D., Zhou, K., McGettigan-Croce, B., Nunez-Cruz, S., Gulendran,, G., Boesteanu, A.C., Johnson, L., Feldman, M.D., Radaelli, E., Mansfield, K., Nasrallah, M., Goydel,, R., Peng, H., Rader, C., Milone, M.C., Siegel, D.L.: Adoptive T cell immunotherapy for medullary thyroid carcinoma targeting GDNF family receptor alpha 4 (GFRa4). Molecular Therapeutic – Oncolytics 20: 387-398, 2021.

Nobles, C., Sherrill-Mix, S., Everett, J.K., Reddy, S., Fraietta, J., Porter, D., Frey, N., Gill, S.I., Grupp, S., Maude, S., Siegel, D.L., Levine, B.L. June, C.H., Lacey, S., Melenhorst, J., Bushman, F.: CAR T cell immunotherapy outcomes correlate with genomic modification by vector integration. J Clin Invest 130: 673-685, 2020.

Choi, H., Kudchodkar, S.B., Reuschel, E.L., Asija, K., Borole, P., Agarwal, S., Van Gorder, L., Reed, C.C., Gulendram, G., Ramos, S., Broderick, K.E., Kim, J.J., Ugen, K.E., Kobinger, G., Siegel, D.L., Weiner, D.B., Muthumani, K.: Synthetic nucleic acid antibody prophylaxis confers rapid and durable protective immunity against Zika virus challenge. Human Vaccines & Immunotherapeutics 16: 907-918, 2020.

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Last updated: 12/11/2021
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