Research Interests
Mammalian gene regulation
cell differentiation
chromatin structure
Key words: chromatin, gene regulation, transcription, differentiation, liver and pancreas development.
Description of Research
The goal of the laboratory is to understand how genes are activated and different cell types are specified in embryonic development. These processes involve regulatory mechanisms that are used later in life to maintain human health, to respond to tissue damage, and during the initiation of cancers and other human diseases. The laboratory has two general approaches. First, we investigate the molecular signaling pathways that commit an undifferentiated embryonic cell, the endoderm, to a particular cell type fate, using the specification of liver and pancreas cells as a model. In the past year, we developed a fate map of the foregut endoderm in the mouse embryo, we discovered how a gene regulatory protein controls morphogenesis so that endoderm cells are properly positioned to receive organ-inductive signals, and we found distinct roles for blood vessel cells in promoting the growth of liver and pancreatic tissues at the earliest stages of organ development. The second approach of the laboratory is to investigate ways that gene regulatory proteins control the packaging of DNA in the cell nucleus, to control gene activity. Biochemical studies revealed that the regulatory protein FoxA possesses a protein segment that interacts with chromosome structural proteins, or histones, and is necessary for exposing genes sequences in chromosomes that are otherwise hidden by the histone proteins. Understanding how regulatory proteins and cell signals control gene activity and cell type decisions in development will help guide future efforts to control the differentiation and function of cells at will.
Rotation Projects for 2009-2011
1. Biochemical and genetic analysis of cell signaling and transcription factor activation in mouse embryo tissues, during liver and pancreas cell specification.
2. Epigenetic regulation of developmental gene expression.
3. Mechanisms of transcription factor modulation of chromatin structure.
4. Genetic lineage tracing of different liver and pancreas progenitors.
5. Basis for pluripotency reprogramming by transcription factor.
Lab personnel:
Deborah Freedman-Cass, Ph.D., Senior Research Investigator
Ewa Wandzioch, Ph.D., Research Associate
Chengran Xu, Ph.D., Research Associate
David Metzger, Ph.D., Postdoctoral Associate
Jungsun Kim, Ph.D., Postdoctoral Associate
Juanma Caravaca, Ph.D., Postdoctoral Associate
Andrea Wecker, B.A., M.S., Research Specialist
Angela Hines, B.S., Research Specialist
Masashi Abe, M.S., Graduate Student
Selected Publications
Sekiya, T., Muthurajan, U.M., Tulin, A., McPherson, C., Luger, K., and Zaret, K.S.: Nucleosome-binding affinity as a primary determinant of the nuclear mobility of pioneer transcription factor FoxA. Genes and Development 23: 804-809, 2009.
Wandzioch, E., and Zaret, K.S.: Dynamic Signaling network for the specification of embryonic pancreas and liver progenitors. Science 324: 1707-1710, 2009.
Zaret Kenneth S: Genetic programming of liver and pancreas progenitors: lessons for stem-cell differentiation. Nature reviews. Genetics 9(5): 329-40, May 2008.
Zaret KS, Watts J, Xu J, Wandzioch E, Smale ST, Sekiya T: Pioneer Factors, Genetic Competence, and Inductive Signaling: Programming Liver and Pancreas Progenitors from the Endoderm. Cold Spring Harbor symposia on quantitative biology Nov 2008.
Zaret Kenneth S, Grompe Markus: Generation and regeneration of cells of the liver and pancreas. Science (New York, N.Y.) 322(5907): 1490-4, Dec 2008.
Sekiya Takashi, Zaret Kenneth S: Repression by Groucho/TLE/Grg proteins: genomic site recruitment generates compacted chromatin in vitro and impairs activator binding in vivo. Molecular cell 28(2): 291-303, Oct 2007.
Calmont Amélie, Wandzioch Ewa, Tremblay Kimberly D, Minowada George, Kaestner Klaus H, Martin Gail R, Zaret Kenneth S: An FGF response pathway that mediates hepatic gene induction in embryonic endoderm cells. Developmental cell 11(3): 339-48, Sep 2006.
Yoshitomi Hideyuki, Zaret Kenneth S: Endothelial cell interactions initiate dorsal pancreas development by selectively inducing the transcription factor Ptf1a. Development (Cambridge, England) 131(4): 807-17, Feb 2004.
Cirillo Lisa Ann, Lin Frank Robert, Cuesta Isabel, Friedman Dara, Jarnik Michal, Zaret Kenneth S: Opening of compacted chromatin by early developmental transcription factors HNF3 (FoxA) and GATA-4. Molecular cell 9(2): 279-89, Feb 2002.
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Last updated: 09/21/2009
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