Michael C. Milone, M.D., Ph.D.

faculty photo
Associate Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
Department: Pathology and Laboratory Medicine
Graduate Group Affiliations

Contact information
3400 Spruce Street 7103 Founders Pavilion
Philadelphia, PA 19104
Office: 215-662-6575
Fax: 215-662-7529
B.S (Chemical Biology)
Stevens Institute of Technology, 1993.
M.D. (Medical Doctor)
UMDNJ- New Jersey Medical School, 1999.
Ph.D (Experimental Pathology/ Immunology)
UMDNJ - Graduate School of Biomedical Sciences, 1999.
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Description of Research Expertise

Research in my laboratory is focused on T cell biology and applications of genetically-engineered T cells to the treatment of disease.

Active research projects in my laboratory include:

1) Developing chimeric antigen receptors (CARs) and other engineering approaches for enhancing the function and improved safety of adoptive T cell therapy.

2) Developing and applying synthetic immunoreceptors to the treatment of immune-mediated disease.

3) Exploring the role of co-stimulatory signals in directing T cell metabolism and the way this metabolism affects T cell function within tumors.

Description of Clinical Expertise

Clinical Laboratory Toxicology, Therapeutic Drug Monitoring, Clinical Chemistry, Blood Banking/Transfusion Medicine, Therapeutic Apheresis

Selected Publications

Thakrar AP, Faude S, Perrone J, Milone MC, Lowenstein M, Snider CK, Spadaro A, Delgado MK, Nelson LS, Kilaru AS.: Association of Urine Fentanyl Concentration With Severity of Opioid Withdrawal Among Patients Presenting to the Emergency Department. J Addict Med Mar 2023.

Garfall AL, Cohen AD, Susanibar-Adaniya SP, Hwang WT, Vogl DT, Waxman AJ, Lacey SF, Gonzalez VE, Fraietta JA, Gupta M, Kulikovskaya I, Tian L, Chen F, Koterba N, Bartoszek RL, Patchin M, Xu R, Plesa G, Siegel DL, Brennan A, Nelson AM, Ferthio R, Cosey A, Shea KM, Leskowitz R, Four M, Wilson WV, Miao F, Lancaster E, Carreno BM, Linette GP, Hexner EO, Young RM, Bu D, Mansfield KG, Brogdon JL, June CH, Milone MC, Stadtmauer EA.: Anti-BCMA/CD19 CAR T Cells with Early Immunomodulatory Maintenance for Multiple Myeloma Responding to Initial or Later-Line Therapy. Blood Cancer Discov 4: 118-133, Mar 2023.

Oh S, Mao X, Manfredo-Vieira S, Lee J, Patel D, Choi EJ, Alvarado A, Cottman-Thomas E, Maseda D, Tsao PY, Ellebrecht CT, Khella SL, Richman DP, O'Connor KC, Herzberg U, Binder GK, Milone MC, Basu S, Payne AS.: Precision targeting of autoantigen-specific B cells in muscle-specific tyrosine kinase myasthenia gravis with chimeric autoantibody receptor T cells. Nat Biotechnol Jan 2023.

Dhodapkar KM, Cohen AD, Kaushal A, Garfall AL, Manalo RJ, Carr AR, McCachren SS, Stadtmauer EA, Lacey SF, Melenhorst JJ, June CH, Milone MC, Dhodapkar MV.: Changes in Bone Marrow Tumor and Immune Cells Correlate with Durability of Remissions Following BCMA CAR T Therapy in Myeloma. Blood Cancer Discov. Aug 2022 Notes: OF1-OF12. doi: 10.1158/2643-3230.BCD-22-0018. Online ahead of Print.

Gill, E. L., Mack, E. A., Osterhoudt, K. C., Shaw, L. M., Milone, M. C.: An Epidemic within a Pandemic: An 8-Year-Old Boy with Xylazine-Complicating Fentanyl Poisoning, and Drug Detection Challenges. J Appl Lab Med 2022.

Zhang, H., Yu, P., Tomar, V. S., Chen, X., Atherton, M. J., Lu, Z., Zhang, H. G., Li, S., Ortiz, A., Gui, J., Leu, N. A., Yan, F., Blanco, A., Meyer-Ficca, M. L., Meyer, R. G., Beiting, D. P., Li, J., Nunez-Cruz, S., O'Connor, R. S., Johnson, L. R., Minn, A. J., George, S. S., Koumenis, C., Diehl, J. A., Milone, M. C., Zheng, H., Fuchs, S. Y.: Targeting PARP11 to avert immunosuppression and improve CAR T therapy in solid tumors. Nat Cancer 3(7): 808-820, 2022.

Song, E. Z., Wang, X., Philipson, B. I., Zhang, Q., Thokala, R., Zhang, L., Assenmacher, C. A., Binder, Z. A., Ming, G. L., O'Rourke, D. M., Song, H., Milone, M. C.: The IAP antagonist birinapant enhances chimeric antigen receptor T cell therapy for glioblastoma by overcoming antigen heterogeneity. Mol Ther Oncolytics 27: 288-304, 2022.

Garfall, A. L., Cohen, A. D., Susanibar-Adaniya, S. P., Hwang, W. T., Vogl, D. T., Waxman, A. J., Lacey, S. F., Gonzalez, V. E., Fraietta, J. A., Gupta, M., Kulikovskaya, I., Tian, L., Chen, F., Koterba, N., Bartoszek, R. L., Patchin, M., Xu, R., Plesa, G., Siegel, D. L., Brennan, A., Nelson, A. M., Ferthio, R., Cosey, A., Shea, K. M., Leskowitz, R., Four, M., Wilson, W. V., Miao, F., Lancaster, E., Carreno, B. M., Linette, G. P., Hexner, E. O., Young, R. M., Bu, D., Mansfield, K. G., Brogdon, J. L., June, C. H., Milone, M. C., Stadtmauer, E. A.: Anti-BCMA/CD19 CAR T cells with early immunomodulatory maintenance for multiple myeloma responding to initial or later-line therapy. Blood Cancer Discov 2022.

Ellis, G. I., Deng, M. Z., Winn, D. W., Coker, K. E., Shukla, D., Bhoj, V., Milone, M. C., Duran-Struuck, R., Riley, J. L.: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation. STAR Protoc 3(4): 101784, 2022.

Ellis, G. I., Coker, K. E., Winn, D. W., Deng, M. Z., Shukla, D., Bhoj, V., Milone, M. C., Wang, W., Liu, C., Naji, A., Duran-Struuck, R., Riley, J. L.: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque. Cell Rep Med 3(5): 100614, 2022.

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Last updated: 04/05/2023
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