Publications

For a complete pubmed list of Kaestner Lab publications, please visit here.

List of selected publications:

2023

  • Mammalian Intestinal Development and Differentiation - the State of the Art. Kolev HM, Kaestner KH. Cellular and Molecular Gastroenterology and Hepatology. 2023

  • Single-cell expression profiling of islets generated by the Human Pancreas Analysis Program. Patil AR, Schug J, Naji A, Kaestner KH, Faryabi RB, Vahedi G. Nature Metabolism. 2023

  • Advanced precision modeling reveals divergent responses of hepatocellular carcinoma to combinatorial immunotherapy. Liu J, Cheng L, El-Mekkoussi H, Assenmacher CA, Lee MYY, Jaffe DR, Garvin-Darby K, Morgan A, Manduchi E, Schug J, Kaestner KH. Cancer Communications. 2023

  • The Role of Non-Coding RNAs in Liver Disease, Injury, and Regeneration. Rowe MM, Kaestner KH. Cells. 2023

2022

  • Kolev HM, Swisa A, Manduchi E, Lan Y, Stine RR, Testa G, Kaestner KH. H3K27me3 Demethylases Maintain the Transcriptional and Epigenomic Landscape of the Intestinal Epithelium. Cellular and Molecular Gastroenterology and Hepatology. 2022
  • Shapira SN, Naji A, Atkinson MA, Powers AC, Kaestner KH. Understanding islet dysfunction in type 2 diabetes through multidimensional pancreatic phenotyping: The Human Pancreas Analysis Program. Cell Metabolism. 2022
  • Chen KH, Doliba N, May CL, Roman J, Ustione A, Tembo T, Negron A, Radovick S, Piston DW, Glaser B, Kaestner KH, Matschinsky FM. Genetic activation of glucokinase in a minority of pancreatic beta cells causes hypoglycemia in mice. Life Sciences. 2022
  • Su C, Gao L, May CL, Pippin JA, Boehm K, Lee M, Liu C, Pahl MC, Golson ML, Naji A; HPAP Consortium; Grant SFA, Wells AD, Kaestner KH. 3D chromatin maps of the human pancreas reveal lineage-specific regulatory architecture of T2D risk. Cell Metabolism. 2022 
  • Doliba NM, Rozo AV, Roman J, Qin W, Traum D, Gao L, Liu J, Manduchi E, Liu C, Golson ML, Vahedi G, Naji A, Matschinsky FM, Atkinson MA, Powers AC, Brissova M, Kaestner KH, Stoffers DA; HPAP Consortium. α Cell dysfunction in islets from nondiabetic, glutamic acid decarboxylase autoantibody-positive individuals. Journal of Clinical Investigation. 2022
  • Fasolino M, Schwartz GW, Patil AR, Mongia A, Golson ML, Wang YJ, Morgan A, Liu C, Schug J, Liu J, Wu M, Traum D, Kondo A, May CL, Goldman N, Wang W, Feldman M, Moore JH, Japp AS, Betts MR; HPAP Consortium; Faryabi RB, Naji A, Kaestner KH, Vahedi G. Single-cell multi-omics analysis of human pancreatic islets reveals novel cellular states in type 1 diabetes. Nature Metabolism. 2022

2021

  • Wu M, Lee MYY, Bahl V, Traum D, Schug J, Kusmartseva I, Atkinson MA, Fan G, HPAP Consortium, Kaestner KH. Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry. Cell Rep. 2021.
  • Kondo A, Ma S, Lee MYY, Ortiz V, Traum D, Schug J, Wilkins B, Terry NA, Lee H, Kaestner KH. Highly Multiplexed Image Analysis of Intestinal Tissue Sections in Patients With Inflammatory Bowel Disease.Gastroenterology. 2021.
  • Kolev HM, Tian Y, Kim MS, Leu NA, Adams-Tzivelekidis S, Lengner CJ, Li N, Kaestner KH. A FoxL1-CreERT-2A-tdTomato Mouse Labels Subepithelial Telocytes. Cell Mol Gastroenterol Hepatol. 2021.
  • Kondo A, Kaestner KH. FoxL1+ mesenchymal cells are a critical source of Wnt5a for midgut elongation during mouse embryonic intestinal development. Cells Dev. 2021.
  • Reizel Y, Morgan A, Gao L, Schug J, Mukherjee S, García MF, Donahue G, Baur JA, Zaret KS, Kaestner KH. FoxA-dependent demethylation of DNA initiates epigenetic memory of cellular identity. Dev Cell. 2021.

  • Bahl V, Lee May C, Perez A, Glaser B, Kaestner KH. Genetic activation of α-cell glucokinase in mice causes enhanced glucose-suppression of glucagon secretion during normal and diabetic states. Mol Metab. 2021.

2020

  • Avrahami D, Wang YJ, Schug J, Feleke E, Gao L, Liu C, HPAP Consortium, Naji A, Glaser B, Kaestner KH. Single cell transcriptomics of human islet ontogeny defines the molecular basis of beta cell dedifferentiation in T2D. Molecular Metabolism. 2020.
  • Reizel Y, Morgan, A, Gao L, Lan Y, Manduchi E, Waite E, Wang AW, Wells A, Kaestner KH. Collapse of the hepatic gene regulatory network in the absence of FoxA factors. Genes & Development. 2020
  • Fasolino M, Goldman N, Wang W, Cattau B, Zhou Y, Petrovic J, Link VM, Cote A, Chandra A, Silverman M, Joyce EF, Little SC; HPAP Consortium, Kaestner KH, Naji A, Raj A, Henao-Mejia J, Faryabi RB, Vahedi G. Genetic variation in Type 1 Diabetes reconfigures the 3D chromatin organization of T cells and alters gene expression. Immunity. 2020
  • Wang AW, Wang YJ, Zahm AM, Morgan AR, Wangensteen KJ, Kaestner KH. The Dynamic chromatin architecture of the gegenerating liver. Cell Mol Gastroenterol Hepatol. 2020
  • Zahm AM, Wang AW, Wan YJ, Schug J, Wangensteen KJ, Kaestner KH. A high-content screen identifies microRNAs that regulate liver repopulation after injury in mice. Gastroenterology. 2020

2019

  • Kaestner KH, Powers AC, Naji A; HPAP Consortium, Atkinson MA. NIH Initiative to improve understanding of the pancreas, islet, and autoimmunity in Type 1 Diabetes: The Human Pancreas Analysis Program (HPAP). Diabetes. 2019
  • Wang YJ, Traum D, Schug J, Gao L, Liu C; HPAP Consortium, Atkinson MA, Powers AC, Feldman MD, Naji A, Chang KM, Kaestner KH.Multiplexed In Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes. Cell Metab. 2019
  • Ou K, Yu M, Moss NG, Wang YJ, Wang AW, Nguyen SC, Jiang C, Feleke E, Kameswaran V, Joyce EF, Naji A, Glaser B, Avrahami D, Kaestner KH.Targeted demethylation at the CDKN1C/p57 locus induces human β cell replication. J Clin Invest. 2019

2018

  • Kameswaran V, Golson ML, Ramos-Rodríguez M, Ou K, Wang YJ, Zhang J, Pasquali L, Kaestner KH. The Dysregulation of the DLK1-MEG3Locus in islets from patients with type 2 Diabetes Is mimicked by targeted epimutation of its promoter with TALE-DNMT constructs. Diabetes. 2018
  • Ackermann AM, Moss NG, Kaestner KH.GABA and Artesunate do not induce pancreatic α-to-β cell transdifferentiation in vivo. Cell Metab. 2018
  • Terry NA, Ngaba LV, Wilkins BJ, Pi D, Gheewala N, Kaestner KH. Lipid malabsorption from altered hormonal signaling changes early gut microbial responses. Am J Physiol Gastrointest Liver Physiol. 2018
  • Reizel Y, Sabag O, Skversky Y, Spiro A, Steinberg B, Bernstein D, Wang A, Kieckhaefer J, Li C, Pikarsky E, Levin-Klein R, Goren A, Rajewsky K, Kaestner KH, Cedar H. Postnatal DNA demethylation and its role in tissue maturation. Nat Commun. 2018
  • Shoshkes-Carmel M, Wang YJ, Wangensteen KJ, Tóth B, Kondo A, Massasa EE, Itzkovitz S, Kaestner KH. Subepithelial telocytes are an important source of Wnts that supports intestinal crypts. Nature. 2018
  • Ou K, Zhang J, Jiao Y, Wang ZV, Scherer P, Kaestner KH. Overexpression of ST5, an activator of Ras, has no effect on β-cell proliferation in adult mice. Mol Metab. 2018
  • Wang AW, Wangensteen KJ, Wang YJ, Zahm AM, Moss NG, Erez N, Kaestner KH.TRAP-seq identifies cystine/glutamate antiporter as a driver of recovery from liver injury. J Clin Invest. 2018
  • Wangensteen KJ, Wang YJ, Dou Z, Wang AW, Mosleh-Shirazi E, Horlbeck MA, Gilbert LA, Weissman JS, Berger SL, Kaestner KH. Combinatorial genetics in liver repopulation and carcinogenesis with a in vivo CRISPR activation platform. Hepatology. 2018

2017

  • Swisa A, Kaestner KH, Dor Y. Transcriptional Noise and Somatic Mutations in the Aging Pancreas. Cell Metab. 2017
  • Ackermann AM, Zhang J, Heller A, Briker A, Kaestner KH. High-fidelity Glucagon-CreERmouse line generated by CRISPR-Cas9 assisted gene targeting. Mol Metab. 2017

2016

  • Kieckhaefer J, Lukovac S, Ye DZ, Lee D, Beetler DJ, Pack M, Kaestner KH.The RNA polymerase III subunit Polr3b is required for the maintenance of small intestinal crypts in mice. Cell Mol Gastroenterol Hepatol. 2016
  • Kim R, Sheaffer KL, Choi I, Won KJ, Kaestner KH.Epigenetic regulation of intestinal stem cells by Tet1-mediated DNA hydroxymethylation. Genes Dev. 2016
  • Wang YJ, Golson ML, Schug J, Traum D, Liu C, Vivek K, Dorrell C, Naji A, Powers AC, Chang KM, Grompe M, Kaestner KH. Single-Cell Mass Cytometry Analysis of the Human Endocrine Pancreas. Cell Metab. 2016
  • Dorrell C, Schug J, Canaday PS, Russ HA, Tarlow BD, Grompe MT, Horton T, Hebrok M, Streeter PR, Kaestner KH, Grompe M. Human islets contain four distinct subtypes of β cells. Nat Commun. 2016
  • Wang YJ, Schug J, Won KJ, Liu C, Naji A, Avrahami D, Golson ML, Kaestner KH. Single-Cell Transcriptomics of the Human Endocrine Pancreas. Diabetes. 2016
  • Shin S, Wangensteen KJ, Teta-Bissett M, Wang YJ, Mosleh-Shirazi E, Buza EL, Greenbaum LE, Kaestner KH. Genetic lineage tracing analysis of the cell of origin of hepatotoxin-induced liver tumors in mice. Hepatology. 2016
  • Ackermann AM, Wang Z, Schug J, Naji A, Kaestner KH. Integration of ATAC-seq and RNA-seq identifies human alpha cell and beta cell signature genes. Mol Metab. 2016
  • Aoki R, Shoshkes-Carmel M, Gao N, Shin S, May CL, Golson ML, Zahm AM, Ray M, Wiser CL, Wright CV, Kaestner KH. Foxl1-expressing mesenchymal cells constitute the intestinal stem cell niche. Cell Mol Gastroenterol Hepatol. 2016
  • Elliott EN, Sheaffer KL, Kaestner KH. The 'de novo' DNA methyltransferase Dnmt3b compensates the Dnmt1-deficient intestinal epithelium. Elife. 2016

2015

  • Ferreira MJ, McKenna LB, Zhang J, Reichert M, Bakir B, Buza EL, Furth EE, Bogue CW, Rustgi AK, Kaestner KH. Spontaneous Pancreatitis Caused by Tissue-Specific Gene Ablation of Hhexin Mice. Cell Mol Gastroenterol Hepatol. 2015
  • Avrahami D, Li C, Zhang J, Schug J, Avrahami R, Rao S, Stadler MB, Burger L, Schübeler D, Glaser B, Kaestner KH. Aging-Dependent Demethylation of Regulatory Elements Correlates with Chromatin State and Improved β Cell Function. Cell Metab. 2015
  • Elliott EN, Sheaffer KL, Schug J, Stappenbeck TS, Kaestner KH. Dnmt1 is essential to maintain progenitors in the perinatal intestinal epithelium. Development. 2015
  • Wangensteen KJ, Zhang S, Greenbaum LE, Kaestner KH. A genetic screen reveals Foxa3 and TNFR1 as key regulators of liver repopulation. Genes Dev. 2015
  • Bernstein DL, Le Lay JE, Ruano EG, Kaestner KH. TALE-mediated epigenetic suppression of CDKN2A increases replication in human fibroblasts. J Clin Invest. 2015