Protocols
Adsorption of Polyclonal Antibodies with Antigen-Negative Cells
Affinity Purification of Antibodies
Calcium Phosphate Transfection of Adherent Cells
Cholesterol Depletion of HeLa Cells
Coomassie Stain with A12 for Mass-Spec
Easy Intracellular Immunofluorescence Microscopy
Fixation Protocol for Platelets
Protocol for FACS Analysis of HeLa Transfectants
Purification of His6-Tagged Proteins (1L cells)
Sedimentation Velocity Through Sucrose Gradients for Determination
Transfection of 293T Cells (Modified CaPO4 Protocol)
Transfection of B- and T- Cell Lines By Electroporation