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Recent Publications

All Ostap Lab Publications on PubMed

A local solution to local load: Myo1E as a tension-responsive actuator in clathrin-mediated endocytosis.
Proceedings of the National Academy of Sciences of the United States of America. 2026 Apr 21;123(16):e2605026123 Epub 2026 Apr 13; Authors: Xu M, Ostap EM
Non-muscle tropomyosins inhibit myosin-19 and dynamically localize to mitochondrially associated actin filaments.
The Journal of biological chemistry. 2026 Mar 18;302(5):111377; Authors: Thompson CP, Pollard LW, Xu M, Holzbaur ELF, Ostap EM
A myosin hypertrophic cardiomyopathy mutation disrupts the super-relaxed state and boosts contractility by enhanced actin attachment.
Proceedings of the National Academy of Sciences of the United States of America. 2025 Dec 30;122(52):e2521561122 Epub 2025 Dec 24; Authors: Cail RC, Barua B, Báez-Cruz FA, Winkelmann DA, Goldman YE, Ostap EM
S2Tag, a novel affinity tag for the capture and immobilization of coiled-coil proteins: Application to the study of human β-cardiac myosin.
The Journal of biological chemistry. 2025 Nov;301(11):110776 Epub 2025 Sep 29; Authors: Barua B, Cail RC, Goldman YE, Ostap EM, Winkelmann DA
A myosin hypertrophic cardiomyopathy mutation disrupts the super-relaxed state and boosts contractility by enhanced actin attachment.
bioRxiv : the preprint server for biology. 2025 Jul 22; Authors: Cail RC, Barua B, Báez-Cruz FA, Winkelmann DA, Goldman YE, Ostap EM
S2Tag, a novel affinity tag for the capture and immobilization of coiled-coil proteins: application to the study of human β-cardiac myosin.
bioRxiv : the preprint server for biology. 2025 May 2; Authors: Barua B, Cail RC, Goldman YE, Ostap EM, Winkelmann DA
Dynamics of β-cardiac myosin between the super-relaxed and disordered-relaxed states.
The Journal of biological chemistry. 2025 May;301(5):108412 Epub 2025 Mar 19; Authors: Cail RC, Báez-Cruz FA, Winkelmann DA, Goldman YE, Ostap EM
High-resolution structures of Myosin-IC reveal a unique actin-binding orientation, ADP release pathway, and power stroke trajectory.
Proceedings of the National Academy of Sciences of the United States of America. 2025 Mar 4;122(9):e2415457122 Epub 2025 Feb 27; Authors: Chavali SS, Carman PJ, Shuman H, Ostap EM, Sindelar CV
High resolution structures of Myosin-IC reveal a unique actin-binding orientation, ADP release pathway, and power stroke trajectory.
bioRxiv : the preprint server for biology. 2025 Jan 30; Authors: Chavali SS, Carman PJ, Shuman H, Ostap EM, Sindelar CV
Myosin-I synergizes with Arp2/3 complex to enhance the pushing forces of branched actin networks.
Science advances. 2024 Sep 13;10(37):eado5788; Authors: Xu M, Rutkowski DM, Rebowski G, Boczkowska M, Pollard LW, Dominguez R, Vavylonis D, Ostap EM
Myosin-1C differentially displaces tropomyosin isoforms altering their inhibition of motility.
The Journal of biological chemistry. 2024 Aug;300(8):107539 Epub 2024 Jul 4; Authors: Pollard LW, Boczkowska M, Dominguez R, Ostap EM
Molecular mechanisms linking missense ACTG2 mutations to visceral myopathy.
Science advances. 2024 May 31;10(22):eadn6615; Authors: Ceron RH, Báez-Cruz FA, Palmer NJ, Carman PJ, Boczkowska M, Heuckeroth RO, Ostap EM, Dominguez R
An interphase actin wave promotes mitochondrial content mixing and organelle homeostasis.
Nature communications. 2024 May 7;15(1):3793; Authors: Coscia SM, Moore AS, Thompson CP, Tirrito CF, Ostap EM, Holzbaur ELF
Myosin-I Synergizes with Arp2/3 Complex to Enhance Pushing Forces of Branched Actin Networks.
bioRxiv : the preprint server for biology. 2024 Feb 12; Authors: Xu M, Rutkowski DM, Rebowski G, Boczkowska M, Pollard LW, Dominguez R, Vavylonis D, Ostap EM
Endocytic myosin-1 is a force-insensitive, power-generating motor.
The Journal of cell biology. 2023 Oct 2;222(10) Epub 2023 Aug 7; Authors: Pedersen RTA, Snoberger A, Pyrpassopoulos S, Safer D, Drubin DG, Ostap EM
Drosophila class-I myosins that can impact left-right asymmetry have distinct ATPase kinetics.
The Journal of biological chemistry. 2023 Aug;299(8):104961 Epub 2023 Jun 26; Authors: Báez-Cruz FA, Ostap EM
204b Endocytic myosin-1 is a force-insensitive, power-generating motor.
bioRxiv : the preprint server for biology. 2023 Jun 30; Authors: Pedersen RT, Snoberger A, Pyrpassopoulos S, Safer D, Drubin DG, Ostap EM
Myo19 tethers mitochondria to endoplasmic reticulum-associated actin to promote mitochondrial fission.
Journal of cell science. 2023 Mar 1;136(5) Epub 2023 Mar 2; Authors: Coscia SM, Thompson CP, Tang Q, Baltrusaitis EE, Rhodenhiser JA, Quintero-Carmona OA, Ostap EM, Lakadamyali M, Holzbaur ELF
Ensembles of human myosin-19 bound to calmodulin and regulatory light chain RLC12B drive multimicron transport.
The Journal of biological chemistry. 2023 Feb;299(2):102906 Epub 2023 Jan 13; Authors: Pollard LW, Coscia SM, Rebowski G, Palmer NJ, Holzbaur ELF, Dominguez R, Ostap EM
KIF1A is kinetically tuned to be a superengaging motor under hindering loads.
Proceedings of the National Academy of Sciences of the United States of America. 2023 Jan 10;120(2):e2216903120 Epub 2023 Jan 4; Authors: Pyrpassopoulos S, Gicking AM, Zaniewski TM, Hancock WO, Ostap EM
Modulation of Kinesin's Load-Bearing Capacity by Force Geometry and the Microtubule Track.
Biophysical journal. 2022 Dec 6;121(23):4748 Epub 2022 Nov 30; Authors: Pyrpassopoulos S, Shuman H, Ostap EM
Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites.
Molecular biology of the cell. 2022 May 15;33(6):ar52 Epub 2021 Oct 27; Authors: Masucci EM, Relich PK, Lakadamyali M, Ostap EM, Holzbaur ELF
Myosin modulators: emerging approaches for the treatment of cardiomyopathies and heart failure.
The Journal of clinical investigation. 2022 Mar 1;132(5); Authors: Day SM, Tardiff JC, Ostap EM
High-Speed Optical Traps Address Dynamics of Processive and Non-Processive Molecular Motors.
Methods in molecular biology (Clifton, N.J.). 2022;2478:513-557; Authors: Gardini L, Woody MS, Kashchuk AV, Goldman YE, Ostap EM, Capitanio M
Microtubule Dumbbells to Assess the Effect of Force Geometry on Single Kinesin Motors.
Methods in molecular biology (Clifton, N.J.). 2022;2478:559-583; Authors: Pyrpassopoulos S, Shuman H, Ostap EM
Cega: a single particle segmentation algorithm to identify moving particles in a noisy system.
Molecular biology of the cell. 2021 Apr 19;32(9):931-941 Epub 2021 Mar 31; Authors: Masucci EM, Relich PK, Ostap EM, Holzbaur ELF, Lakadamyali M
Myosin with hypertrophic cardiac mutation R712L has a decreased working stroke which is rescued by omecamtiv mecarbil.
eLife. 2021 Feb 19;10; Authors: Snoberger A, Barua B, Atherton JL, Shuman H, Forgacs E, Goldman YE, Winkelmann DA, Ostap EM
The mechanochemistry of the kinesin-2 KIF3AC heterodimer is related to strain-dependent kinetic properties of KIF3A and KIF3C.
Proceedings of the National Academy of Sciences of the United States of America. 2020 Jul 7;117(27):15632-15641 Epub 2020 Jun 22; Authors: Bensel BM, Woody MS, Pyrpassopoulos S, Goldman YE, Gilbert SP, Ostap EM
The regulatory protein 14-3-3β binds to the IQ motifs of myosin-IC independent of phosphorylation.
The Journal of biological chemistry. 2020 Mar 20;295(12):3749-3756 Epub 2019 Dec 6; Authors: Ji HH, Ostap EM
Modulation of Kinesin's Load-Bearing Capacity by Force Geometry and the Microtubule Track.
Biophysical journal. 2020 Jan 7;118(1):243-253 Epub 2019 Dec 12; Authors: Pyrpassopoulos S, Shuman H, Ostap EM
Single molecule mechanics resolves the earliest events in force generation by cardiac myosin.
eLife. 2019 Sep 17;8; Authors: Woody MS, Winkelmann DA, Capitanio M, Ostap EM, Goldman YE
Motors in transport and cytoskeleton remodeling.
Molecular biology of the cell. 2019 Mar 15;30(6):734; Authors: Welburn JPI, Ostap EM
Opening remarks from the Editors.
Biophysical reviews. 2018 Dec;10(6):1479-1480; Authors: De La Cruz EM, Blanchoin L, Ostap EM
Molecular to organismal chirality is induced by the conserved myosin 1D.
Science (New York, N.Y.). 2018 Nov 23;362(6417):949-952; Authors: Lebreton G, Géminard C, Lapraz F, Pyrpassopoulos S, Cerezo D, Spéder P, Ostap EM, Noselli S
Positive cardiac inotrope omecamtiv mecarbil activates muscle despite suppressing the myosin working stroke.
Nature communications. 2018 Sep 21;9(1):3838; Authors: Woody MS, Greenberg MJ, Barua B, Winkelmann DA, Goldman YE, Ostap EM
Electro-optic deflectors deliver advantages over acousto-optical deflectors in a high resolution, ultra-fast force-clamp optical trap.
Optics express. 2018 Apr 30;26(9):11181-11193; Authors: Woody MS, Capitanio M, Ostap EM, Goldman YE
High-resolution cryo-EM structures of actin-bound myosin states reveal the mechanism of myosin force sensing.
2004 Proceedings of the National Academy of Sciences of the United States of America. 2018 Feb 6;115(6):1292-1297 Epub 2018 Jan 22; Authors: Mentes A, Huehn A, Liu X, Zwolak A, Dominguez R, Shuman H, Ostap EM, Sindelar CV
Opposing Kinesin and Myosin-I Motors Drive Membrane Deformation and Tubulation along Engineered Cytoskeletal Networks.
Current biology : CB. 2018 Jan 22;28(2):236-248.e5 Epub 2018 Jan 11; Authors: McIntosh BB, Pyrpassopoulos S, Holzbaur ELF, Ostap EM
Adhesion force and attachment lifetime of the KIF16B-PX domain interaction with lipid membranes.
Molecular biology of the cell. 2017 Nov 7;28(23):3315-3322 Epub 2017 Sep 20; Authors: Pyrpassopoulos S, Shuman H, Ostap EM
Deconvolution of Camera Instrument Response Functions.
Biophysical journal. 2017 Mar 28;112(6):1214-1220; Authors: Lewis JH, Jamiolkowski RM, Woody MS, Ostap EM, Goldman YE
Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers.
Methods in molecular biology (Clifton, N.J.). 2017;1486:483-509; Authors: Greenberg MJ, Shuman H, Ostap EM
Activity-Dependent Regulation of Distinct Transport and Cytoskeletal Remodeling Functions of the Dendritic Kinesin KIF21B.
Neuron. 2016 Nov 23;92(4):857-872 Epub 2016 Nov 3; Authors: Ghiretti AE, Thies E, Tokito MK, Lin T, Ostap EM, Kneussel M, Holzbaur ELF
MEMLET: An Easy-to-Use Tool for Data Fitting and Model Comparison Using Maximum-Likelihood Estimation.
Biophysical journal. 2016 Jul 26;111(2):273-282; Authors: Woody MS, Lewis JH, Greenberg MJ, Goldman YE, Ostap EM
Myosin-I molecular motors at a glance.
Journal of cell science. 2016 Jul 15;129(14):2689-95 Epub 2016 Jul 11; Authors: McIntosh BB, Ostap EM
A Perspective on the Role of Myosins as Mechanosensors.
Biophysical journal. 2016 Jun 21;110(12):2568-2576; Authors: Greenberg MJ, Arpağ G, Tüzel E, Ostap EM
Structural Analysis of the Myo1c and Neph1 Complex Provides Insight into the Intracellular Movement of Neph1.
Molecular and cellular biology. 2016 Jun 1;36(11):1639-54 Epub 2016 May 16; Authors: Arif E, Sharma P, Solanki A, Mallik L, Rathore YS, Twal WO, Nath SK, Gandhi D, Holzman LB, Ostap EM, Ashish, Nihalani D
Force Generation by Membrane-Associated Myosin-I.
Scientific reports. 2016 May 9;6:25524; Authors: Pyrpassopoulos S, Arpağ G, Feeser EA, Shuman H, Tüzel E, Ostap EM
An Actin Filament Population Defined by the Tropomyosin Tpm3.1 Regulates Glucose Uptake.
Traffic (Copenhagen, Denmark). 2016 Jan;17(1):80-1; Authors: Kee AJ, Yang L, Lucas CA, Greenberg MJ, Martel N, Leong GM, Hughes WE, Cooney GJ, James DE, Ostap EM, Han W, Gunning PW, Hardeman EC
An actin filament population defined by the tropomyosin Tpm3.1 regulates glucose uptake.
Traffic (Copenhagen, Denmark). 2015 Jul;16(7):691-711 Epub 2015 Apr 29; Authors: Kee AJ, Yang L, Lucas CA, Greenberg MJ, Martel N, Leong GM, Hughes WE, Cooney GJ, James DE, Ostap EM, Han W, Gunning PW, Hardeman EC
Mechanochemical tuning of myosin-I by the N-terminal region.
Proceedings of the National Academy of Sciences of the United States of America. 2015 Jun 30;112(26):E3337-44 Epub 2015 Jun 8; Authors: Greenberg MJ, Lin T, Shuman H, Ostap EM
WHAMM Directs the Arp2/3 Complex to the ER for Autophagosome Biogenesis through an Actin Comet Tail Mechanism.
Current biology : CB. 2015 Jun 29;25(13):1791-7 Epub 2015 Jun 18; Authors: Kast DJ, Zajac AL, Holzbaur EL, Ostap EM, Dominguez R
Control of the initiation and termination of kinesin-1-driven transport by myosin-Ic and nonmuscle tropomyosin.
Current biology : CB. 2015 Feb 16;25(4):523-9 Epub 2015 Feb 5; Authors: McIntosh BB, Holzbaur EL, Ostap EM