Recent Publications

All Ostap Lab Publications on PubMed

• Dynamics of β-cardiac myosin between the super-relaxed and disordered-relaxed states.
  The Journal of biological chemistry. 2025 Mar 19;301(5):108412; Authors: Cail RC, Báez-Cruz FA, Winkelmann DA, Goldman YE, Ostap EM

• High-resolution structures of Myosin-IC reveal a unique actin-binding orientation, ADP release pathway, and power stroke trajectory.
  Proceedings of the National Academy of Sciences of the United States of America. 2025 Mar 4;122(9):e2415457122 Epub 2025 Feb 27; Authors: Chavali SS, Carman PJ, Shuman H, Ostap EM, Sindelar CV

• High resolution structures of Myosin-IC reveal a unique actin-binding orientation, ADP release pathway, and power stroke trajectory.
  bioRxiv : the preprint server for biology. 2025 Jan 30; Authors: Chavali SS, Carman PJ, Shuman H, Ostap EM, Sindelar CV

• Myosin-I synergizes with Arp2/3 complex to enhance the pushing forces of branched actin networks.
  Science advances. 2024 Sep 13;10(37):eado5788; Authors: Xu M, Rutkowski DM, Rebowski G, Boczkowska M, Pollard LW, Dominguez R, Vavylonis D, Ostap EM

• Myosin-1C differentially displaces tropomyosin isoforms altering their inhibition of motility.
  The Journal of biological chemistry. 2024 Aug;300(8):107539 Epub 2024 Jul 4; Authors: Pollard LW, Boczkowska M, Dominguez R, Ostap EM

• Molecular mechanisms linking missense ACTG2 mutations to visceral myopathy.
  Science advances. 2024 May 31;10(22):eadn6615; Authors: Ceron RH, Báez-Cruz FA, Palmer NJ, Carman PJ, Boczkowska M, Heuckeroth RO, Ostap EM, Dominguez R

• An interphase actin wave promotes mitochondrial content mixing and organelle homeostasis.
  Nature communications. 2024 May 7;15(1):3793; Authors: Coscia SM, Moore AS, Thompson CP, Tirrito CF, Ostap EM, Holzbaur ELF

• Myosin-I Synergizes with Arp2/3 Complex to Enhance Pushing Forces of Branched Actin Networks.
  bioRxiv : the preprint server for biology. 2024 Feb 12; Authors: Xu M, Rutkowski DM, Rebowski G, Boczkowska M, Pollard LW, Dominguez R, Vavylonis D, Ostap EM

• Endocytic myosin-1 is a force-insensitive, power-generating motor.
  The Journal of cell biology. 2023 Oct 2;222(10) Epub 2023 Aug 7; Authors: Pedersen RTA, Snoberger A, Pyrpassopoulos S, Safer D, Drubin DG, Ostap EM

• Drosophila class-I myosins that can impact left-right asymmetry have distinct ATPase kinetics.
  The Journal of biological chemistry. 2023 Aug;299(8):104961 Epub 2023 Jun 26; Authors: Báez-Cruz FA, Ostap EM

• Endocytic myosin-1 is a force-insensitive, power-generating motor.
  bioRxiv : the preprint server for biology. 2023 Jun 30; Authors: Pedersen RT, Snoberger A, Pyrpassopoulos S, Safer D, Drubin DG, Ostap EM

• Myo19 tethers mitochondria to endoplasmic reticulum-associated actin to promote mitochondrial fission.
  Journal of cell science. 2023 Mar 1;136(5) Epub 2023 Mar 2; Authors: Coscia SM, Thompson CP, Tang Q, Baltrusaitis EE, Rhodenhiser JA, Quintero-Carmona OA, Ostap EM, Lakadamyali M, Holzbaur ELF

• Ensembles of human myosin-19 bound to calmodulin and regulatory light chain RLC12B drive multimicron transport.
  The Journal of biological chemistry. 2023 Feb;299(2):102906 Epub 2023 Jan 13; Authors: Pollard LW, Coscia SM, Rebowski G, Palmer NJ, Holzbaur ELF, Dominguez R, Ostap EM

• KIF1A is kinetically tuned to be a superengaging motor under hindering loads.
  Proceedings of the National Academy of Sciences of the United States of America. 2023 Jan 10;120(2):e2216903120 Epub 2023 Jan 4; Authors: Pyrpassopoulos S, Gicking AM, Zaniewski TM, Hancock WO, Ostap EM

• Modulation of Kinesin's Load-Bearing Capacity by Force Geometry and the Microtubule Track.
  Biophysical journal. 2022 Dec 6;121(23):4748 Epub 2022 Nov 30; Authors: Pyrpassopoulos S, Shuman H, Ostap EM

• Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites.
  Molecular biology of the cell. 2022 May 15;33(6):ar52 Epub 2021 Oct 27; Authors: Masucci EM, Relich PK, Lakadamyali M, Ostap EM, Holzbaur ELF

• Myosin modulators: emerging approaches for the treatment of cardiomyopathies and heart failure.
  The Journal of clinical investigation. 2022 Mar 1;132(5); Authors: Day SM, Tardiff JC, Ostap EM

• High-Speed Optical Traps Address Dynamics of Processive and Non-Processive Molecular Motors.
  Methods in molecular biology (Clifton, N.J.). 2022;2478:513-557; Authors: Gardini L, Woody MS, Kashchuk AV, Goldman YE, Ostap EM, Capitanio M

• Microtubule Dumbbells to Assess the Effect of Force Geometry on Single Kinesin Motors.
  Methods in molecular biology (Clifton, N.J.). 2022;2478:559-583; Authors: Pyrpassopoulos S, Shuman H, Ostap EM

• Cega: a single particle segmentation algorithm to identify moving particles in a noisy system.
  Molecular biology of the cell. 2021 Apr 19;32(9):931-941 Epub 2021 Mar 31; Authors: Masucci EM, Relich PK, Ostap EM, Holzbaur ELF, Lakadamyali M

• Myosin with hypertrophic cardiac mutation R712L has a decreased working stroke which is rescued by omecamtiv mecarbil.
  eLife. 2021 Feb 19;10; Authors: Snoberger A, Barua B, Atherton JL, Shuman H, Forgacs E, Goldman YE, Winkelmann DA, Ostap EM

• The mechanochemistry of the kinesin-2 KIF3AC heterodimer is related to strain-dependent kinetic properties of KIF3A and KIF3C.
  Proceedings of the National Academy of Sciences of the United States of America. 2020 Jul 7;117(27):15632-15641 Epub 2020 Jun 22; Authors: Bensel BM, Woody MS, Pyrpassopoulos S, Goldman YE, Gilbert SP, Ostap EM

• The regulatory protein 14-3-3β binds to the IQ motifs of myosin-IC independent of phosphorylation.
  The Journal of biological chemistry. 2020 Mar 20;295(12):3749-3756 Epub 2019 Dec 6; Authors: Ji HH, Ostap EM

• Modulation of Kinesin's Load-Bearing Capacity by Force Geometry and the Microtubule Track.
  Biophysical journal. 2020 Jan 7;118(1):243-253 Epub 2019 Dec 12; Authors: Pyrpassopoulos S, Shuman H, Ostap EM

• Single molecule mechanics resolves the earliest events in force generation by cardiac myosin.
  eLife. 2019 Sep 17;8; Authors: Woody MS, Winkelmann DA, Capitanio M, Ostap EM, Goldman YE

• Motors in transport and cytoskeleton remodeling.
  Molecular biology of the cell. 2019 Mar 15;30(6):734; Authors: Welburn JPI, Ostap EM

• Opening remarks from the Editors.
  Biophysical reviews. 2018 Dec;10(6):1479-1480; Authors: De La Cruz EM, Blanchoin L, Ostap EM

• Molecular to organismal chirality is induced by the conserved myosin 1D.
  Science (New York, N.Y.). 2018 Nov 23;362(6417):949-952; Authors: Lebreton G, Géminard C, Lapraz F, Pyrpassopoulos S, Cerezo D, Spéder P, Ostap EM, Noselli S

• Positive cardiac inotrope omecamtiv mecarbil activates muscle despite suppressing the myosin working stroke.
  Nature communications. 2018 Sep 21;9(1):3838; Authors: Woody MS, Greenberg MJ, Barua B, Winkelmann DA, Goldman YE, Ostap EM

• Electro-optic deflectors deliver advantages over acousto-optical deflectors in a high resolution, ultra-fast force-clamp optical trap.
  Optics express. 2018 Apr 30;26(9):11181-11193; Authors: Woody MS, Capitanio M, Ostap EM, Goldman YE

• High-resolution cryo-EM structures of actin-bound myosin states reveal the mechanism of myosin force sensing.
  Proceedings of the National Academy of Sciences of the United States of America. 2018 Feb 6;115(6):1292-1297 Epub 2018 Jan 22; Authors: Mentes A, Huehn A, Liu X, Zwolak A, Dominguez R, Shuman H, Ostap EM, Sindelar CV

• Opposing Kinesin and Myosin-I Motors Drive Membrane Deformation and Tubulation along Engineered Cytoskeletal Networks.
  Current biology : CB. 2018 Jan 22;28(2):236-248.e5 Epub 2018 Jan 11; Authors: McIntosh BB, Pyrpassopoulos S, Holzbaur ELF, Ostap EM

• Adhesion force and attachment lifetime of the KIF16B-PX domain interaction with lipid membranes.
  Molecular biology of the cell. 2017 Nov 7;28(23):3315-3322 Epub 2017 Sep 20; Authors: Pyrpassopoulos S, Shuman H, Ostap EM

• Deconvolution of Camera Instrument Response Functions.
  Biophysical journal. 2017 Mar 28;112(6):1214-1220; Authors: Lewis JH, Jamiolkowski RM, Woody MS, Ostap EM, Goldman YE

• Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers.
  Methods in molecular biology (Clifton, N.J.). 2017;1486:483-509; Authors: Greenberg MJ, Shuman H, Ostap EM

• Activity-Dependent Regulation of Distinct Transport and Cytoskeletal Remodeling Functions of the Dendritic Kinesin KIF21B.
  Neuron. 2016 Nov 23;92(4):857-872 Epub 2016 Nov 3; Authors: Ghiretti AE, Thies E, Tokito MK, Lin T, Ostap EM, Kneussel M, Holzbaur ELF

• MEMLET: An Easy-to-Use Tool for Data Fitting and Model Comparison Using Maximum-Likelihood Estimation.
  Biophysical journal. 2016 Jul 26;111(2):273-282; Authors: Woody MS, Lewis JH, Greenberg MJ, Goldman YE, Ostap EM

• Myosin-I molecular motors at a glance.
  Journal of cell science. 2016 Jul 15;129(14):2689-95 Epub 2016 Jul 11; Authors: McIntosh BB, Ostap EM

• A Perspective on the Role of Myosins as Mechanosensors.
  Biophysical journal. 2016 Jun 21;110(12):2568-2576; Authors: Greenberg MJ, Arpağ G, Tüzel E, Ostap EM

• Structural Analysis of the Myo1c and Neph1 Complex Provides Insight into the Intracellular Movement of Neph1.
  Molecular and cellular biology. 2016 Jun 1;36(11):1639-54 Epub 2016 May 16; Authors: Arif E, Sharma P, Solanki A, Mallik L, Rathore YS, Twal WO, Nath SK, Gandhi D, Holzman LB, Ostap EM, Ashish, Nihalani D

• Force Generation by Membrane-Associated Myosin-I.
  Scientific reports. 2016 May 9;6:25524; Authors: Pyrpassopoulos S, Arpağ G, Feeser EA, Shuman H, Tüzel E, Ostap EM

• An Actin Filament Population Defined by the Tropomyosin Tpm3.1 Regulates Glucose Uptake.
  Traffic (Copenhagen, Denmark). 2016 Jan;17(1):80-1; Authors: Kee AJ, Yang L, Lucas CA, Greenberg MJ, Martel N, Leong GM, Hughes WE, Cooney GJ, James DE, Ostap EM, Han W, Gunning PW, Hardeman EC

• An actin filament population defined by the tropomyosin Tpm3.1 regulates glucose uptake.
  Traffic (Copenhagen, Denmark). 2015 Jul;16(7):691-711 Epub 2015 Apr 29; Authors: Kee AJ, Yang L, Lucas CA, Greenberg MJ, Martel N, Leong GM, Hughes WE, Cooney GJ, James DE, Ostap EM, Han W, Gunning PW, Hardeman EC

• Mechanochemical tuning of myosin-I by the N-terminal region.
  Proceedings of the National Academy of Sciences of the United States of America. 2015 Jun 30;112(26):E3337-44 Epub 2015 Jun 8; Authors: Greenberg MJ, Lin T, Shuman H, Ostap EM

• WHAMM Directs the Arp2/3 Complex to the ER for Autophagosome Biogenesis through an Actin Comet Tail Mechanism.
  Current biology : CB. 2015 Jun 29;25(13):1791-7 Epub 2015 Jun 18; Authors: Kast DJ, Zajac AL, Holzbaur EL, Ostap EM, Dominguez R

• Control of the initiation and termination of kinesin-1-driven transport by myosin-Ic and nonmuscle tropomyosin.
  Current biology : CB. 2015 Feb 16;25(4):523-9 Epub 2015 Feb 5; Authors: McIntosh BB, Holzbaur EL, Ostap EM